文章摘要
吴旻娜,王浩,李悠然,王微,谷云飞,贡钰霞.枳术方对慢传输型便秘大鼠结肠5-HT4R及下游CaM-MLCK信号通路的影响[J].南京中医药大学学报,2021,37(6):891-896.
枳术方对慢传输型便秘大鼠结肠5-HT4R及下游CaM-MLCK信号通路的影响
Effect of Zhizhu Decoction on 5-HT4R and Downstream CAM-MLCK Signaling Pathway in Colon of Rats with Slow Transit Constipation
  
DOI:
中文关键词: 关键词:枳术方  慢传输型便秘  5-HT4受体  钙调蛋白  肌球蛋白轻链激酶
英文关键词: Zhizhu Decoction  slow transit constipation  5-HT4R  calmodulin  myosin light chain kinase
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作者单位
吴旻娜,王浩,李悠然,王微,谷云飞,贡钰霞 南京中医药大学附属医院江苏 南京 210029 
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中文摘要:
      目的 通过观察枳术方对慢传输型便秘(STC)大鼠结肠组织中5-羟色胺4受体(5-HT4R)、钙调蛋白(CaM)、肌球蛋白轻链激酶(MLCK)的影响,探讨其作用机制。方法 将大鼠随机分为正常组、模型组、枳术方组及阳性药(普芦卡必利)组。采用复方地芬诺酯片建立STC模型,枳术方组随后灌胃枳术方,阳性药组灌胃普芦卡必利。检测各组大鼠粪便含水量,肠道炭末推进率,结肠组织中5-HT4R、CaM、MLCK的mRNA及蛋白相对表达水平。结果 与正常组相比,模型组大鼠粪便含水量和炭末推进率明显降低(P<0.05,P<0.01),结肠组织中5-HT4R、CaM、MLCK的mRNA和蛋白相对表达水平也明显降低(P<0.01)。与模型组相比,枳术方组和阳性药组大鼠粪便含水量和炭末推进率明显升高(P<0.05),结肠组织中5-HT4R、CaM、MLCK的mRNA和蛋白相对表达水平明显升高(P<0.05,P<0.01)。枳术方组与阳性药组比较,各项数据差异无统计学意义(P>0.05)。结论 枳术方能改善STC大鼠的便秘症状,可能与上调大鼠结肠组织中5-HT4R表达并激活下游CaM-MLCK信号通路有关。
英文摘要:
      OBJECTIVE To observe the effects of Zhizhu Decoction on 5-HT4R、CaM、MLCK in colon tissue of rats with slow transit constipation (STC). METHODS Rats were divided into normal group,model group, Zhizhu Decoction group and positive (prucalopride) group, with 10 rats in each group. STC rats replicated by intragastric administration with compound diphenoxylate tablets. After modeling, Zhizhu Decoction and prucalopride were given, respectively, to corresponding groups by gavage. Water content of feces, the percentage of pushing carbonic powder, mRNA and protein contents of 5-HT4R, CaM and MLCK in colon tissues of different groups were detected. RESULTS Compared with the normal group, the water content of feces and the percentage of pushing carbonic powder in model group significantly decreased (P<0.05,P<0.01), the mRNA and protein contents of 5-HT4R, CaM and MLCK in colon tissues also significantly decreased (P<0.01). Compared with model group, water content of feces and percentage of pushing carbonic powder in Zhizhu Decoction group and positive group significantly increased (P<0.05), and the mRNA and protein expressions of 5-HT4R, CaM and MLCK in colon tissues also significantly increased (P<0.05,P<0.01). The data had no statistical significance between the Zhizhu Decoction group and the positive group (P>0.05). CONCLUSION Zhizhu Decoction can improve the symptoms of STC, which may be related to promoting high expression of 5-HT4R in colon tissue of rats to active the CaM-MLCK signaling pathway.
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