文章摘要
贾跃军,左文仁,苏学林,汤井源,何垚澍,王世衍,苏健,周恩超,朱清毅.复方益肾解毒汤对大鼠急性肾缺血再灌注损伤的机制研究[J].南京中医药大学学报,2020,36(4):494-497.
复方益肾解毒汤对大鼠急性肾缺血再灌注损伤的机制研究
Protective Effect and Mechanism of Compound Yishen Jiedu Decoction on Acute Renal Ischemia-Reperfusion Injury in Rats
  
DOI:
中文关键词: 关键词:益肾解毒汤  肾缺血再灌注损伤  NF-κB  加速康复
英文关键词: Yishen Jiedu decoction  renal ischemia-reperfusion injury  NF-κB  rapid rehabilitation
基金项目:
作者单位
贾跃军1,2,左文仁2,苏学林2,汤井源2,何垚澍2,王世衍2,苏健2,周恩超2,朱清毅2 1.镇江市中西医结合医院泌尿外科江苏 镇江 212000
2.南京中医药大学附属医院/江苏省中医院江苏 南京 210029 
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中文摘要:
      目的 探讨益肾解毒汤对大鼠急性肾缺血再灌注的保护作用及机制研究。方法 48只雄性大鼠随机分为4组:假手术组、肾缺血再灌注组(RIR组)、益肾解毒汤+肾缺血再灌注组(Drug+RIR组)、姜黄素+肾缺血再灌注组(Cur+RIR组)。术后24、96 h取大鼠目内眦采血,比较各组大鼠血清中肌酐(Scr)、尿素氮(BUN)水平;取术后24 h大鼠肾脏组织,比较各组肾脏损伤评分;比较假手术组、RIR组和Drug+RIR组3组炎症因子NF-κB水平。结果 与假手术组比较,RIR组、Drug+RIR组和Cur+RIR组血清Scr、BUN、肾小管损伤评分均升高,Drug+RIR组高于Cur+RIR组,但均明显低于RIR组(P<0.05);且比较术后96 h RIR组、Drug+RIR组2组血清Scr、BUN,Drug+RIR组肾功能指标恢复更快。结论 复方益肾解毒汤对大鼠肾脏缺血再灌注损伤有明显保护作用,其机制可能与NF-κB相关炎症因子的表达有关。
英文摘要:
      OBJECTIVE To investigate the protective effect and mechanism of compound Yishen Jiedu decoction on acute renal ischemia-reperfusion injury in rats. METHODS 48 male rats were randomly divided into four groups: sham surgery group, renal ischemia-reperfusion group (RIR group), Yishen jiedu decoction plus renal ischemia-reperfusion group (Drug+RIR group), and curcumin plus renal ischemia-reperfusion group (Cur+RIR group). Blood samples were collected from the fundus vein at 24 h and 96 h after surgery to compare the serum levels of creatinine (Scr) and urea nitrogen (BUN) in each group. The renal tissues were collected 24 h after surgery, and the renal injury scores of each group were compared. NF-κB levels in Sham surgery group, RIR group and Drug+RIR group were compared. RESULTS Compared with the sham surgery group, serum Scr, BUN, and renal tubular injury scores in RIR group, Drug+RIR group and Cur+RIR group were all increased. Serum Scr, BUN and renal injury scores of the Drug+RIR group were higher than those of the Cur+RIR group, but were significantly lower than those of the RIR group (P<0.05). And compared the serum Scr and BUN of 96h affer surgery, the renal function of the Drug+RIR group recovered faster than RIR group. CONCLUSION Compound Yishen Jiedu decoction has obvious protective effect on renal ischemia-reperfusion injury in rats, and its mechanism may be related to NF-κB related inflammatory factors.
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