文章摘要
黄倩,刘永刚,李京涛,魏海梁,闫曙光,张海博,常占杰.消木丹颗粒对非酒精性脂肪肝大鼠FXR、ApoM表达的影响[J].南京中医药大学学报,2020,36(4):478-484.
消木丹颗粒对非酒精性脂肪肝大鼠FXR、ApoM表达的影响
Effects of Xiaomudan Granules on the Expressions of FXR and ApoM in Rats with Non-Alcoholic Fatty Liver Disease
  
DOI:
中文关键词: 关键词:消木丹颗粒  非酒精性脂肪肝  FXR  ApoM  脂质代谢
英文关键词: Xiaomudan Granule  non-alcoholic fatty liver disease  FXR  ApoM  lipid metabolism
基金项目:
作者单位
黄倩1,刘永刚2,李京涛2,魏海梁2,闫曙光1,张海博3,常占杰2 1.陕西中医药大学第一临床医学院陕西 咸阳 7124062.陕西中医药大学附属医院陕西 咸阳 7124063.大连医科大学医学科学研究院辽宁 大连 116000 
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中文摘要:
      目的 探讨消木丹颗粒对非酒精性脂肪肝(NAFLD)模型大鼠的治疗作用,并探讨其对FXR/ApoM表达的影响。方法 60只SD大鼠按体质量分为正常组,模型组,消木丹颗粒低、中、高剂量组,多烯磷脂酰胆碱组,除正常组外,其余50只采用高脂膳食喂养12周建立NAFLD大鼠模型,造模成功后灌胃给予相应药物,给药4周后取血清及肝脏组织样本。称取大鼠体质量及肝湿质量,计算肝脏指数;HE染色、油红O染色观察肝脏病理变化;ELISA法检测血脂及肝功能水平;免疫组化观察肝组织FXR、ApoM的表达;qPCR法检测肝组织FXR、ApoM mRNA表达水平,Western blot法检测肝脏上述各项基因的蛋白表达。结果 与模型组比较,消木丹颗粒干预后能显著改善NAFLD大鼠体质量、肝脏指数,降低血清ALT、AST、TC、TG、LDL-C含量,增高HDL-C含量,同时能增强肝脏FXR、ApoM的表达,差异有统计学意义(P<0.05~0.01)。结论 消木丹颗粒可调节NAFLD大鼠肝脏的脂质代谢,改善脂质沉积,其机制可能与调控FXR及其相关基因ApoM有关。
英文摘要:
      OBJECTIVE To investigate the therapeutic effect of Xiaomudan Granule in rats with non-alcoholic fatty liver (NAFLD) and to observe the effect of Xiaomudan Granule on the expression of Farnesoid X receptor (FXR) and apolipoprotein M (ApoM), and to explore its mechanism. METHODS A total of 60 SD rats were divided into normal group, model group, Xiaomudan Granule treatment groups (low, middle and high treatment group), polyene phosphatidylcholine group according to body weight. Except the normal group, the remaining 50 rats were treated with high fat diet for 12 weeks to induce NAFLD rat model. After the success of the model, the corresponding drugs were given by i.g. administration. Serum lipids and liver tissue were taken after 4-week of administration. Body mass and liver wet weight of rats were measured and liver index was calculated. HE and Oil Red O staining were used to observe the pathological changes of liver tissue. Serum lipids and liver function were detected by ELISA. The expression levels of FXR and ApoM in liver tissues were observed by immunohistochemistry, the mRNA expression levels of FXR and ApoM in liver tissues were detected by qPCR technology. The protein expression levels of the above-mentioned genes in liver were detected by Western blot. RESULTS After the intervention of Xiaomudan Granule, the levels of ALT, AST, TC, TG and LDL-C were significantly reduced and HDL-C was increased. The mRNA expressions of FXR and ApoM in the liver were enhanced, compared with the model group (P<0.05, P<0.01). CONCLUSION Xiaomudan Granule can regulate lipid metabolism and improve lipid deposition in NAFLD rat liver, and the mechanism may be related to the regulation of FXR and its related gene ApoM.
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