文章摘要
张正光,刘冰,梁炜锋,沈存思.槐耳颗粒逆转肝癌细胞对索拉非尼耐药的初步研究[J].南京中医药大学学报,2020,36(1):83-87.
槐耳颗粒逆转肝癌细胞对索拉非尼耐药的初步研究
Preliminary Study on Reversal of Sorafenib Resistance of Hepatocellular Carcinoma Cells by Huaier Granule
  
DOI:
中文关键词: 关键词:槐耳颗粒  索拉非尼  肝癌  耐药
英文关键词: Huaier granule  Sorafenib  hepatocellular carcinoma  drug resistance
基金项目:
作者单位
张正光1,刘冰1,梁炜锋1,沈存思2 1.南京中医药大学医学院·整合医学学院江苏 南京 210023
2.南京中医药大学附属医院江苏省儿童呼吸疾病中医药重点实验室江苏 南京 210029 
摘要点击次数: 24
全文下载次数: 32
中文摘要:
      目的 初步探讨槐耳颗粒对索拉非尼(Sorafenib)耐药肝癌细胞BEL-7402/S的作用及其相关机制。方法 CCK-8法测定槐耳颗粒对BEL-7402/S细胞的增殖-毒性作用以及对Sorafenib的耐药逆转倍数;qPCR及Western blot法检测槐耳颗粒对BEL-7402/S细胞的缺氧诱导因子-1α(HIF-1α)及血管内皮生长因子(VEGF) mRNA和蛋白表达水平的影响。结果 槐耳颗粒能够抑制BEL-7402/S细胞的活性;在无显著细胞毒性的剂量下,槐耳颗粒能够部分逆转BEL-7402/S细胞对Sorafenib的耐药,其耐药逆转倍数为1.75;qPCR结果显示槐耳颗粒可以下调BEL-7402/S细胞中HIF-1α的mRNA水平,但对VEGF的mRNA水平无显著性影响;Western blot结果显示槐耳颗粒可以降低HIF-1α、VEGF的蛋白表达水平。结论 槐耳颗粒可能具有部分逆转BEL-7402/S细胞对Sorafenib耐药的作用,其机制可能与降低HIF-1α以及VEGF的表达水平有关。
英文摘要:
      OBJECTIVE To investigate the effect and related mechanism of Huaier granule on Sorafenib-resistant hepatocellular carcinoma cells BEL-7402/S. METHODS The proliferation-toxicity effect of Huaier granule on BEL-7402/S cells and the reversal multiple of drug resistance to Sorafenib were measured by CCK-8 assays. The effects of Huaier granule on the mRNA and protein levels of hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) in BEL-7402/S cells were detected by qPCR and Western blot, respectively. RESULTS Huaier granule inhibited the activities of BEL-7402/S cells, and partially reversed the resistance of BEL-7402/S cells to Sorafenib at the dose of no significant cytotoxicity, and the reversal multiple of drug resistance was 1.75; the results of qPCR showed that Huaier granule may down-regulate the mRNA level of HIF-1α in BEL-7402/S cells, but had no significant effect on the mRNA level of VEGF. The results of Western blot showed that Huaier granule may down-regulate the protein levels of HIF-1α and VEGF in BEL-7402/S cells. CONCLUSION Huaier granule may partially reverse the drug resistance of BEL-7402/S cells to Sorafenib, and its mechanism may be related to the decrease of the expression of HIF-1α and VEGF.
查看全文   查看/发表评论  下载PDF阅读器
关闭
微信公众号