文章摘要
吴浩然,黄晓燕,孙广达,蒋睿智,单鑫.炮制对栀子抗抑郁作用的影响及其机制研究[J].南京中医药大学学报,2020,36(1):51-55.
炮制对栀子抗抑郁作用的影响及其机制研究
Anti-Depressant Effect and Its Mechanism of Gardenia Jasminoides Ellis Before and After Processing
  
DOI:
中文关键词: 关键词:栀子  抗抑郁  炮制  慢性不可预知性应激  炎性因子
英文关键词: Gardenia jasminoides Ellis  anti-depressant effect  processing  CUMS  inflammatory factors
基金项目:
作者单位
吴浩然1,黄晓燕1,孙广达1,蒋睿智1,单鑫1,2 1.南京中医药大学中医学院·中西医结合学院江苏 南京 2100232.南京中医药大学翰林学院江苏 泰州 225300 
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中文摘要:
      目的 以栀子不同炮制品为研究对象,比较不同炮制品栀子抗抑郁作用及其机制。方法 建立慢性不可预知性应激(CUMS)小鼠模型,并采用栀子不同炮制品水提物干预;给药结束后,采用行为学评价其抗抑郁作用的差异,并探讨其分子学机制。结果 与模型组相比,炒、焦、生栀子组小鼠糖水消耗显著升高(P<0.01);炒、焦栀子明显降低悬尾试验(P<0.05)和强迫游泳试验(P<0.05~0.01)的不动时间,生栀子对以上2者无显著改善;小鼠海马组织中炎性因子白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α),磷酸化核因子-κB(p-NF-κB)(P<0.01)以及胶质纤维酸性蛋白(GFAP)(P<0.01)的表达在造模后显著上升,炮制后栀子(炒、焦)抗炎作用优于生栀子。其中炒栀子抗炎作用最优,IL-β、TNF-α和GFAP均能被炒栀子显著下调(P<0.01)。结论 栀子不同炮制品抗抑郁作用存在差异,其抗抑郁作用差异与其调节小鼠海马组织中炎性因子IL-1β、TNF-α等以及GFAP的表达有关。
英文摘要:
      OBJECTIVE To investigate the difference of anti-depressant effect between different processed products of Gardenia jasminoides Ellis, and to explore the mechanism. METHODS Chronic unpredictable mild stress model was established and water extract of different processed products of Gardenia jasminoides Ellis was used for administration. Behavioral tests were used to evaluate the difference of anti-depressant effect between different processed products of Gardenia jasminoides Ellis, and the molecular mechanism was further explored. RESULTS The immobility time of TST (P<0.05) and FST (P<0.05,P<0.01) was significantly reduced by CZZ and JZZ while there was no significant improvement in TST and FST in SZZ group. As expected, the expressions of IL-1β,TNF-α, p-NF-κB and GFAP were obviously higher in model group (P<0.01) than those in CZZ and JZZ. CZZ had the best anti-inflammatory effect by significantly decreasing the expressions of IL-1β, TNF-α and GFAP. CONCLUSION The anti-depressant effects of different processed products of Gardenia jasminoides Ellis are different. It is assumed that the mechanism of the anti-depressant effects and its difference is related to the regulation of the expression of IL-1β, TNF-α and GFAP in the hippocampal tissue of mice.
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