文章摘要
黄莉莉,王欣,李冰冰,缪明星.Alopecurone B逆转人乳腺癌细胞MCF-7阿霉素耐药株活性研究[J].南京中医药大学学报,2019,35(6):699-703.
Alopecurone B逆转人乳腺癌细胞MCF-7阿霉素耐药株活性研究
Reversal Effect and Mechanism of Alopecurone B on MCF-7 Adriamycin-Resistant Subline
  
DOI:
中文关键词: 关键词:Alopecurone B  阿霉素  乳腺癌  多药耐药  P-糖蛋白
英文关键词: Alopecurone B  adriamycin  breast cancer  multidrug resistance  P-glycoprotein
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作者单位
黄莉莉1,王欣1,李冰冰1,缪明星2 1.南京大学医学院附属鼓楼医院江苏 南京 2100082.中国药科大学药学院江苏 南京 210009 
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中文摘要:
      目的 研究黄酮化合物Alopecurone B(APB)对人乳腺癌细胞阿霉素耐药株MCF-7/ADR多药耐药的逆转活性及逆转机制。方法 人乳腺癌细胞阿霉素耐药株MCF-7/ADR维持在含有250 ng/mL阿霉素的培养基中,使用MTT法、qPCR、Western blot和流式细胞术研究APB对耐药株P-糖蛋白(P-gp)功能和表达的影响。结果 APB能逆转MCF-7/ADR细胞多药耐药,抑制P-gp的功能,下调P-gp基因和蛋白的表达。结论 APB具有强效逆转人乳腺癌细胞MCF-7/ADR多药耐药的效果,其机制涉及对P-gp的调控。
英文摘要:
      OBJECTIVE To study the reversal effect and the mechanism of APB on adriamycin (ADR)-induced multidrug-resistant human breast cancer MCF-7/ADR cells. METHODS ADR-resistant MCF-7 cells were maintained in RPMI-1640 culture medium containing 10%FBS and 250 ng/mL ADR. MTT assay, qPCR, Western blot and flow cytometry were used to determine the inhibitory effect of APB on P-gp levels and function in MCF-7 or MCF-7/ADR cells. RESULTS APB reversed MCF-7/ADR multidrug resistance at the concentration of 10 μmol/L. Treated with 0, 5, 10, 20 μmol/L of ABP, P-gp gene and protein levels were suppressed significantly in a concentration-dependent manner. Furthermore, APB in 10 μmol/L markedly inhibited the function of P-gp. CONCLUSION APB potent reversed the multidrug resistance in MCF-7 ADR-resistant subline. The mechanism referred to P-gp inhibition.
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