文章摘要
刘睿,陈建亚,朱振华,张燕,钱大玮,段金廒.山羊角中巯基的测定及山羊角对体内巯基的干预研究[J].南京中医药大学学报,2018,34(6):617-620.
山羊角中巯基的测定及山羊角对体内巯基的干预研究
Determination of Thiol in Caprae Hircus Cornu and Its Effect on Thiol Level in Rat Plasma
  
DOI:
中文关键词: 关键词:山羊角  巯基  血浆  测定
英文关键词: Caprae hircus cornu  thiol  plasma  determination
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作者单位
刘睿1,2,3,陈建亚4,朱振华1,3,张燕5,钱大玮1,3,段金廒1,3* 1.南京中医药大学江苏省中药资源产业化过程协同创新中心中药资源产业化与方剂创新药物国家地方联合工程研究中心江苏 南京 2100232.江苏省海洋药用生物资源研究与开发重点实验室江苏 南京 2100233.江苏省方剂高技术研究重点实验室江苏 南京 2100234.江苏生命科技创新园江苏 南京 2100335.南京师范大学公共管理学院江苏 南京 210023 
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中文摘要:
      目的 测定山羊角提取液及大鼠血浆中游离巯基(-SH)与总-SH的含量,探讨山羊角对大鼠体内-SH干预情况。方法 采用Ellman法测定山羊角提取液中游离-SH与总-SH的含量;大鼠连续灌胃给予山羊角提取物后,测定大鼠血浆中游离-SH和总-SH的水平;大鼠灌胃7 d给予山羊角提取物后,连续测定给药后大鼠血浆中游离-SH含量随时间变化情况。结果 山羊角提取液中的游离-SH含量介于3.96~5.08 mmol/L,总-SH的含量介于4.52~5.94 mmol/L;连续给予大鼠山羊角提取液后,第4天始,大鼠血浆中游离-SH与总-SH水平相较于正常大鼠显著提升(P<0.01),而阿司匹林对大鼠血浆中的游离-SH与总-SH水平无影响;口服给予大剂量山羊角后30 min,大鼠血浆中游离-SH含量即达最大值(10.16±1.18)mmol/L,8 h后大鼠血浆中游离-SH含量基本降至正常大鼠水平。结论 山羊角提取液中含有丰富的-SH,且口服山羊角后血浆游离-SH与总-SH水平均显著提升,提示山羊角中含SH基团的一类成分可能为山羊角重要功效物质基础,为角类动物药功效物质基础及效应特点研究提供借鉴与思路。
英文摘要:
      OBJECTIVE To determine the level of native thiols (-SH) and total -SH in goat horn extract and rat plasma. METHODS The thiols levels in goat horn extract and rat plasma were measured using the classical Ellman reagent method. Both the levels of native -SH and total -SH in rat plasma were measured after oral administration of goat horn extract. After oral administration of goat horn extract, the native -SH level in rat plasma was measured, and the curve of -SH level against time after administration was obtained. RESULTS The level of native -SH in goat horn extract ranged from 3.96 to 5.08 mmol/L, and the level of total -SH in goat horn extract ranged from 4.52-5.94 mmol/L. After continuously oral administration of goat horn extract, both the levels of native -SH and total -SH in rat plasma significantly increased (P<0.01) compared with normal rat from d4 to d7. Oral administration of Aspirin did not significantly increase the -SH level in rat plasma. In addition, 30 min after oral administration of goat horn extract, the level of native -SH in rat plasma significantly increased to (10.16±1.18)mmol/L, and 8 h later, the level of native -SH in plasma reverted to normal level. CONCLUSION There are abundant -SH contained components in goat horn extract, and goat horn extract could obviously increase the -SH level in rat plasms. The results in the present study suggested that -SH group contained components might be the important material basis of goat horn. It provides a reference for investigations on animal horn derived TCMs.
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