文章摘要
张钰1,鲁丹1,倪阳1,郑远茹1,姚志红1,2,张丹3,戴毅1,2,于洋1,2,姚新生1,2*.栀子活性部位GJ-4的HPLC指纹图谱及主成分含量测定研究[J].南京中医药大学学报,2018,34(4):416-420.
栀子活性部位GJ-4的HPLC指纹图谱及主成分含量测定研究
Study on HPLC Fingerprint and Content Determination of Major Costituents of GJ-4,an Effective Fraction from Gardeniae Fructus
  
DOI:
中文关键词: 栀子  藏红花素  HPLC  指纹图谱  含量测定
英文关键词: Gardeniae fructus  crocin  HPLC  fingerprint  content determination
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作者单位
张钰1,鲁丹1,倪阳1,郑远茹1,姚志红1,2,张丹3,戴毅1,2,于洋1,2,姚新生1,2* 1.暨南大学中药及天然药物研究所广东 广州 5106322.中药药效物质基础及创新药物研究广东省高校重点实验室广东 广州 5106323.中国医学科学院药物研究所天然药物活性物质与功能国家重点实验室北京 100050 
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中文摘要:
      目的 建立GJ-4 HPLC指纹图谱,并明确特征色谱峰的化学成分,测定主含有效成分藏红花苷-3的含量。方法 采用Phenomenex Gemini C18色谱柱(250 mm×4.6 mm,5 μm),以乙腈-0.1%甲酸水为流动相进行梯度洗脱,检测波长440 nm,建立GJ-4的HPLC指纹图谱,通过相似度软件对其进行相似度分析;对主要有效成分藏红花苷-3进行含量测定。结果 建立了GJ-4的HPLC指纹图谱,对17个主要特征峰进行明确的化学指认,20批GJ-4的指纹图谱相似度在0.939~0.994之间,藏红花苷-3的含量在0.200~0.683 mg/g之间。结论 该方法专属性强,准确度高,可作为GJ-4质量控制的有效方法之一。
英文摘要:
      OBJECTIVE To establish a HPLC fingerprint of GJ-4 (an effective fraction from Gardeniae Fructus) and to quantify the main component crocin-3. METHODS The experiment was carried out on a Phenomenex Gemini C18 column (250 mm×4.6 mm, 5 μm), eluted with ACN-water with 1‰ formic gradiently and determined at 440 nm wavelength. HPLC fingerprint chromatogram of GJ-4 was set up and quality of 20 batches of GJ-4 was evaluated by similarity assay. The major contents of crocin-3 was determined. RESULTS The HPLC fingerprint chromatogram of GJ-4 was been established, in which 17 compounds were identified. The similarity of 20 batches of GJ-4 was about 0.939 to 0.994, and the content of crocin-3 was about 0.200 to 0.682 mg/g. CONCLUSION The method established in this paper can provide a comprehensive reference for the quality control of GJ-4.
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