文章摘要
成颖1,吴娟1,章聪1,姚学权2,杨学文2,杨亚平3,张军峰1*,詹瑧1*.新发胃癌患者舌苔类型与舌苔菌群的相关性分析[J].南京中医药大学学报,2018,34(4):404-408.
新发胃癌患者舌苔类型与舌苔菌群的相关性分析
Correlation between the Variety of Tongue Coating and Their Bacterial Community in the New Patients with Gastric Cancer
  
DOI:
中文关键词: 胃癌  苔色  苔质  菌群结构
英文关键词: gastric cancer  coating color  coating nature  bacterial community
基金项目:
作者单位
成颖1,吴娟1,章聪1,姚学权2,杨学文2,杨亚平3,张军峰1*,詹瑧1* 1. 南京中医药大学医学与生命科学学院江苏 南京 2100232. 南京中医药大学附属医院江苏 南京 2100293. 南京中医药大学基础医学院江苏 南京 210023 
摘要点击次数: 1031
全文下载次数: 748
中文摘要:
      目的 探讨舌苔形成的微生态学机制。方法 新发胃癌患者47例作为病例组,健康体检者42例作为对照组,年龄性别匹配,基于16S rRNA基因进行454高通量焦磷酸测序检测舌苔菌群,统计分析苔色、苔质与舌苔菌群的相关性。结果 在病例组和对照组中,不同舌苔的菌群丰富度、多样性均无显著差异。病例组和对照组分别有31个(1纲、6目、9科和14属)和 10个(1目、3科和6属)分类单元具有苔色显著差异(P<0.05),然而仅有5个(1目、1科和3属)和2个(1科和1属)分类单元具有苔质显著差异(P<0.05)。其中,黄苔慢生根瘤菌科(Bradyrhizobiaceae)和代尔夫特菌属(Delftia)相对丰度均显著高于白苔(P<0.05)。舌苔菌群共生关系显示,苔色、苔质组间比组内拥有更高比例的显著负相关。基于舌苔菌群进行功能预测,胃癌黄苔患者嘌呤代谢显著高于白苔患者(P<0.05),与血清尿酸水平一致;对照组厚苔人群脂质代谢显著高于薄苔人群(P<0.05),与血清总胆固醇水平一致。结论 舌苔菌群结构与苔色相关,菌群共生关系可能反映了中医舌苔辨证的科学内涵。
英文摘要:
      OBJECTIVE To investigate the correlation between the formation of tongue coating and the tongue coating flora. METHODS A hospital-based case-control study was developed with 47 new patients with gastric cancer (GC) as cases and 42 healthy people as controls. The tongue coating flora was measured by 454 high-throughput pyrosequencing based on 16S rRNA gene. Multi-factor correlated analysis was conducted between the tongue coating and the tongue coating flora. RESULTS The richness and diversity of the tongue coating flora showed no correlation with the coating color or the coating nature in both the cases and controls. 31 taxa (1 classes, 6 orders, 9 families and 14 genera) and 10 taxa (1 orders, 3 families and 6 genera) showed significantly different between the white coating and yellow coating in the cases and controls, respectively (P<0.05). However, only 5 taxa (1 order, 1 family and 3 genera) and 2 taxa (1 family and 1 genus) were observed significantly different between thin coating and thick coating in the cases and controls, respectively (P<0.05). The relative abundances of Bradyrhizobiaceae and Delftia in the yellow coating were distinctly higher than that in the white coating (P<0.05). The ratio of significantly negative correlations of the bacterial genera between the white coating and yellow coating was obviously higher than that in the white coating or that in the yellow coating, and similar phenomenon was observed in the tongue nature. Based on the bacterial function prediction of the tongue coating, the function purine metabolism was significantly higher in GC patients with yellow coating than that with white coating (P<0.05), which was confirmed by the serum uric acid level. Meanwhile, the function lipid metabolism was stronger in the controls with thick coating than that in the controls with thin coating (P<0.05), which was confirmed by the serum total cholesterol level. CONCLUSION The structure of tongue coating flora shows stronger impact on the coating color than the coating nature, and the symbiotic correlation in the tongue coating flora may reflect the scientific connotation of TCM syndrome differentiation of tongue coating.
查看全文   查看/发表评论  下载PDF阅读器
关闭
微信公众号