文章摘要
葛永亮 1,2 ,陶金华 2 ,吴木军 1*.黄芩苷对辐射诱导的小鼠急性肺损伤保护作用机制研究[J].南京中医药大学学报,2017,33(6):613-617.
黄芩苷对辐射诱导的小鼠急性肺损伤保护作用机制研究
Effect of Baicalin on Radiation-Induced Acute Lung Injury in Mice Through Antioxidant and Anti-Inflammatory Properties
  
DOI:
中文关键词: 黄芩苷  辐射  急性肺损伤  NF-κBp65
英文关键词: baicalin  radiation  acute lung injury  NF-κBp65
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作者单位
葛永亮 1,2 ,陶金华 2 ,吴木军 1* 1.南通市通州区人民医院影像科江苏 南通 2260002.南通大学药学院江苏 南通 226001 
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中文摘要:
      目的 探讨黄芩苷对辐射诱导小鼠的急性肺损伤保护作用。方法 X-线复制小鼠的急性肺损动物模型。将50只ICR小鼠随机分为空白对照组、X-线组、X-线组+地塞米松组(5mg/kg)、X-线组+黄芩苷组(20、40mg/kg)。观察各组肺组织病理学改变,测量肺湿/干质量比,支气管肺泡灌洗液中性粒细胞百分比、肺组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、炎症因子白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)含量及肺组织中p-NFκBp65、p-IκBα蛋白表达。结果 黄芩苷组(20、40mg/kg)可有效减轻辐射所致肺组织病理学变化,能降低肺泡灌洗液中炎症细胞的数目,能显著降低肺湿/干质量比重,能提高SOD水平,并能降低MDA水平,降低p-NFκBP65和p-IκBα蛋白的表达。结论 黄芩苷可减轻辐射所致急性肺组织损伤,对辐射诱导的急性肺损伤有保护作用。
英文摘要:
      OBJECTIVE The current study was designed to evaluate the protective effects of baicalin on radiation-induced acute lung injury in mice. METHODS 50 ICR mice were divided into five groups randomly, namely, control group, X-ray group, X-ray+dexamethasone group (5mg/kg), X-ray+baicalin group (20, 40 mg/kg), with 8 in each group. Pathological changes in the lung tissues were observed. The ratio of lung wet/dry weight was also recorded. Besides, the level of neutrophil in bronchoalveolar lavage fluid (BALF) was detected to evaluate inflammatory cell infiltration. Moreover, the expressions of MDA, IL-6, TNF-α, p-NFκBp65 and p-IκBα, as well as the activity of SOD, were examined in lung. RESULTS Baicalin (20, 40 mg/kg) could effectively improve the radiation-induced lung pathological changes and reduce the number of inflammatory cells in BALF. The ratio of lung wet/dry weight was also decreased with the treatment of baicalin (20, 40 mg/kg). In addition, the activity of SOD was recovered and the end product of lipid peroxidation MDA was reduced meanwhile. The expressions of p-NFκBp65 and p-IκBα was extensively down-regulated with baicalin (20, 40 mg/kg) preconditioning, taking the expressions of NFκBp65 and IκBα as inner controls. CONCLUSION Baicalin could alleviate radiation-induced acute lung injury due to its antioxidant and anti-inflammatory properties.
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