文章摘要
张爱凤,盛玉青,邹明畅.泽泻醇B抗4T1乳腺癌细胞侵袭转移的体外研究[J].南京中医药大学学报,2018,34(2):178-180.
泽泻醇B抗4T1乳腺癌细胞侵袭转移的体外研究
Investigation on the Anti-Metastatic Effect of Alisol B Isolated from Alismaorientale on 4T1 Breast Cancer Cells in Vitro
投稿时间:2017-09-11  
DOI:
中文关键词: 泽泻醇B  乳腺癌  泽泻  转移  基质金属蛋白酶
英文关键词: Alisol B  breast cancer  Alismaorientale  metastasis  matrix metalloproteinases
基金项目:
作者单位
张爱凤1,盛玉青2,邹明畅2* 1.镇江市第四人民医院药剂科江苏 镇江 
212002
2.镇江市第一人民医院药剂科 江苏 镇江 
212002 
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中文摘要:
      探讨泽泻醇B(Alisol B)对乳腺癌细胞株4T1转移能力的抑制作用及可能机制。方法 4T1细胞经药物作用24 h后,通过MTT法检测药物对细胞生长的影响;通过划痕试验及Transwell小室检测泽泻醇B对细胞侵袭和迁移能力的影响;通过Western blot 检测细胞经药物作用后对基质金属蛋白酶MMP-2及MMP-9蛋白表达水平的变化。结果 泽泻醇B对4T1细胞的生长具有一定的抑制作用。划痕实验显示泽泻醇B可显著抑制乳腺癌细胞的转移能力,细胞经药物(10 μmol/L)作用24 h后,其相对迁移率降至(52.66±8.48)%(P<0.01)。Transwell小室侵袭实验同样证实泽泻醇B能够有效抑制乳腺癌细胞的转移,经药物(10 μmol/L)作用24 h后,24 h内细胞相对侵袭力下降至(47.06±9.32)%(P<0.01)。Western blot结果表明,泽泻醇B能够使癌细胞内MMP-2及MMP-9蛋白表达量显著下调。结论 泽泻醇B在体外能够有效抑制4T1乳腺癌细胞的转移,其机制可能与抑制细胞内基质金属蛋白酶的表达量有关。
英文摘要:
      OBJECTIVE To investigate the anti-metastatic effect of Alisol B on 4T1 breast cancer cells and explore its possible mechanisms. METHODS 4T1 breast cancer cells were treated with Alisol B for 24 h. Cell viability was evaluated by MTT assay. Migration ability was evaluated by wound healing assay. Invasion ability was investigated by transwell invasion assay. The expressions of matrix metalloproteinases (MMP-2 and MMP-9) were detected by Western blot analysis. RESULTS Alisol B showed inhibitory effect on cell growth in 4T1 cells. Wound healing assay demonstrated that Alisol B inhibited the migration of 4T1 cells after the treatment for 24 h. The migration rate was (52.66±8.48)% (P<0.01). Transwell invasion assay proved that Alisol B effectively suppressed the invasion of 4T1 cells.The invasion rate was (47.06±9.32)% (P<0.01). Western blot results demonstrated that the expression of MMP-2 and MMP-9 were significantly down-regulated with the treatment of Alisol B. CONCLUSION Alisol B can suppress the metastasis of 4T1 breast cancer cells in vitro, which could be through down-regulation the expression of matrix metalloproteinases.
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