文章摘要
朱慧,刘晓,祝婷婷,汪小莉,朱晓钗,蔡宝昌.熟大黄水煎液中化学成分含量测定及其药效学研究[J].南京中医药大学学报(自然科学版),2017,33(5):470-475.
熟大黄水煎液中化学成分含量测定及其药效学研究
Determination of the Related Components in Prepared Rhubarb Decoction and Its Pharmacodynamics Study
投稿时间:2017-04-11  修订日期:2017-07-19
DOI:
中文关键词: 熟大黄  含量测定  高效液相色谱法  热毒血瘀证  血液流变学
英文关键词: Prepared rhubarb  Assay  HPLC  Noxious heat blood stasis syndrome  Hemorheology
基金项目:
作者单位
朱慧1,2,刘晓1,2,祝婷婷1,2,汪小莉1,2,朱晓钗1,2,蔡宝昌1,2,3* 1.南京中医药大学药学院江苏 南京 2100232.国家教育部中药炮制规范化及标准化工程研究中心江苏省中药炮制重点实验室江苏 南京 2100233.南京海昌中药集团有限公司江苏 南京 210061 
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中文摘要:
      目的 建立熟大黄水煎液中主要化学成分的含量测定方法并观察其对热毒血瘀模型大鼠的治疗作用。方法 采用Hypersil GOLD C18柱,以0.1%磷酸水(A)-乙腈(B)进行梯度洗脱,检测波长为254nm,柱温30℃,在此条件下进行方法学验证及水煎液样品含量测定。给予热毒血瘀模型大鼠熟大黄水煎剂,取其腹主动脉血液检测以获得血液流变学参数,并测定血浆中血栓素B2(TXB2)、6-酮前列腺素(6-keto-PGF)的含量。结果 所建立的含量测定方法在一定浓度范围内线性关系良好,精密度、重复性、稳定性及回收率均符合相关要求。熟大黄水煎液能显著降低模型大鼠的全血粘度(P<0.05)、血浆粘度(P<0.01)和血液中纤维蛋白原含量(P<0.01),TT(P<0.01)和PT(P<0.01)值显著延长,同时TXB2水平显著降低(P<0.05)而6-keto-PGF水平显著升高(P<0.05),TXB2/6-keto-PGF含量比值极显著下降(P<0.01)被纠正趋向正常。结论 该方法简便、准确可靠,可用于熟大黄水煎液的质量评价与控制。熟大黄能有效改善热毒血瘀模型大鼠血液流变学状态,促进PGI2分泌同时减少TXA2的生成,纠正TXB2/6-keto-PGF1α比值失衡,从而改善血管内皮功能并恢复血液循环,起到活血化瘀的作用。
英文摘要:
      OBJECTIVE To develop an HPLC method for determination of main components in prepared rhubarb decoction and investigate its effects on rats with noxious heat blood stasis syndrome. METHODS The analysis was performed on a Hypersil GOLD C18 column with the mobile phase of 0.1% phosphoric acid solution(A)-acetonitrile(B). The detection wavelength was set at 254nm and the column temperature was 30℃. The method was validated and the samples were determined under this condition. The abdominal aortic blood of rats with noxious heat blood stasis syndrome was collected and tested to obtain the hemorheological parameters. Also, the TXB2 and 6-keto-PGF levels in rats plasma were measured. RESULTS The developed HPLC method showed good linearity within certain concentration ranges. And the precision, repeatability, stability and recovery all met the requirements. Compared with rats in model group, the whole blood viscosity at four shear rates(P<0.05), plasma viscosity(P<0.01) and the level of plasma fibrinogen content(P<0.01) in the high dose group were significantly decreased after doing, and the TT(P<0.01) and PT(P<0.01) values were significantly prolonged. Furthermore, the levels of TXB2(decreased, P<0.05) and 6-keto-PGF1α(increased,P<0.05)were significantly changed, and their ratio was corrected to be normal. CONCLUSION The method was simple, accurate and reliable,which could be used for quality control of prepared rhubarb decoction. Prepared rhubarb could improve vascular endothelial function by modifying blood rheology, promoting the secretion of PGI2, reducing the generation of TXA2 and recovering the balance of TXB2/6-keto-PGF1α.
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