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核壳型Fe3O4@PDA@Au纳米材料对水牛角含巯基肽类成分的富集研究

刘婷 韩疏影 康安 邓海山 朱栋 池玉梅 刘睿

刘婷, 韩疏影, 康安, 邓海山, 朱栋, 池玉梅, 刘睿. 核壳型Fe3O4@PDA@Au纳米材料对水牛角含巯基肽类成分的富集研究[J]. 南京中医药大学学报, 2020, 36(3): 396-400.
引用本文: 刘婷, 韩疏影, 康安, 邓海山, 朱栋, 池玉梅, 刘睿. 核壳型Fe3O4@PDA@Au纳米材料对水牛角含巯基肽类成分的富集研究[J]. 南京中医药大学学报, 2020, 36(3): 396-400.
LIUTing, HANShu-ying, KANGAn, DENGHai-shan, ZHUDong, CHIYu-mei, LIURui. Enrichment of Thiol-Containing Peptides in Water Buffalo Horn by Core-Shell Fe3O4@PDA@Au nano-Materials[J]. Journal of Nanjing University of traditional Chinese Medicine, 2020, 36(3): 396-400.
Citation: LIUTing, HANShu-ying, KANGAn, DENGHai-shan, ZHUDong, CHIYu-mei, LIURui. Enrichment of Thiol-Containing Peptides in Water Buffalo Horn by Core-Shell Fe3O4@PDA@Au nano-Materials[J]. Journal of Nanjing University of traditional Chinese Medicine, 2020, 36(3): 396-400.

核壳型Fe3O4@PDA@Au纳米材料对水牛角含巯基肽类成分的富集研究

Enrichment of Thiol-Containing Peptides in Water Buffalo Horn by Core-Shell Fe3O4@PDA@Au nano-Materials

  • 摘要: 目的 通过制备核壳型Fe3O4@PDA@Au纳米材料对水牛角中含巯基(-SH)肽类成分进行富集、鉴定与表征。方法 采用种子生长法制备核壳型Fe3O4@PDA@Au磁性纳米材料,将材料应用于水牛角提取液含-SH肽类成分的富集;分别采用Ellman法和多肽试剂盒测定富集前后溶液中-SH及多肽的含量;采用nano LC-MS/MS系统分析鉴定水牛角提取液肽类组成。结果 Fe3O4@PDA@Au纳米材料对水牛角含-SH肽类成分的富集因子为23.41,水牛角提取液中共鉴定了4 114个肽段,其中含有半胱氨酸(Cys)的肽段共11个;富集液中共鉴定了2 254个肽段,其中含有Cys的肽段共709个,富集后的含Cys的肽段与总肽段的比值从0.003增加到0.314,表明材料对水牛角提取物中含-SH肽段完成了较好的富集。结论 Fe3O4@PDA@Au纳米材料对水牛角中含巯基肽类成分具有良好的富集效果,对水牛角功效物质基础研究具有重要意义。

     

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  • 刊出日期:  2020-05-10

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