基于网络药理学的金水六君煎治疗慢性阻塞性肺疾病作用机制研究
Mechanism of Jinshui Liujun Decoction in the Treatment of Chronic Obstructive Pulmonary Disease Based on Network Pharmacology
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摘要: 目的 利用网络药理学探索金水六君煎治疗慢性阻塞性肺疾病(COPD)的作用机制。方法 在TCMSP平台检索该方化学成分及作用靶点,通过TTD、CTD、DisGeNET、GeneCards数据库获得COPD相关靶点,将成分靶点与COPD相关靶点相对应,筛选交集靶点及功效成分。利用STRING数据库和Cytoscape3.7.2构建成分-靶点网络。利用OmicShare平台进行基因功能(GO)和基于京都基因与基因组百科全书(KEGG)富集分析。结果 得到交集靶点96个,功效成分435个(其中主要成分有槲皮素、豆甾醇、山萘酚、β-谷固醇、柚皮苷、熊果酸等20个);自由度、介数和中心性大于平均值的靶点有20个,包括MAPK3、TNF、PPAR-γ、SIRTI、HMOX1、MAPK8、CAT、SIRT1、STAT3等。富集分析显示该方主要涉及对生物和化学刺激的反应、多种细胞生物过程、细胞增殖、免疫、代谢等;IL-17、Toll样受体、TNF、HIF-1、FoxO、TGF-β、ErbB等17条通路在COPD治疗中发挥作用。结论 金水六君煎可通过多成分、多靶点、多通路的协同作用来治疗COPD,为深入探讨金水六君煎治疗COPD的作用机制提供了基础资料。Abstract: OBJECTIVE To explore the mechanism of Jinshui Liujun decoction in the treatment of chronic obstructive pulmonary disease (COPD) by network pharmacology. METHODS The chemical ingredients and corresponding targets of Jinshui Liujun decoction were retrieved from TCMSP platform. The relevant targets of COPD were obtained through the TTD, DisGeNET, GeneCardsand and CTD databases, the targets of drug chemical ingredients were mapped to the targets of COPD. The intersection targets and the efficacy components were obtained. Using STRING database and Cytoscape 3.7.2 software, component-target network model was constructed. The OmicShare open platform was used to perform gene function (GO) and the Kyoto Gene and Genome Encyclopedia (KEGG) enrichment analysis. RESULTS Jinshui Liujun decoction had 96 intersection targets for COPD, 435 efficacy components (The main components are quercetin, stigmasterol, kaempferol, β-sitosterol, naringin, ursolic acid, etc.), and 20 key targets which degree,betweenness and closeness are greater than average, including MAPK3, TNF, PPAR-γ, SIRTI, HMOX1, MAPK8, CAT, SIRT1, STAT3, etc. Enrichment analysis showed that the formula mainly involved the response to biological and chemical stimuli, various cell biological processes, cell proliferation, immunity, metabolism, etc. 17 signaling pathways including IL-17, Toll-like receptor, TNF, HIF-1, FoxO, TGF-β and ErbB, etc. play a role in the treatment of COPD. CONCLUSION Jinshui Liujun decoction can treat COPD through the synergy of multiple ingredients, multiple targets and multiple pathways, which provides basic data for in-depth exploration of the mechanism of Jinshui Liu jun decoction in the treatment of COPD.
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