基于HPLC指纹图谱的半夏及其伪品鉴别研究
Identification of Pinellia Ternata<\i> and Its Counterfeits Based on HPLC Fingerprint Analysis
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摘要: 目的 建立高效液相色谱法(HPLC)对半夏及其伪品进行指纹图谱分析,以鉴定半夏的真伪。方法 用SB-Aq色谱柱(4.6 mm×250 mm,5 μm),以0.2%磷酸二氢铵溶液-甲醇为流动相进行梯度洗脱,进样量20 μL,流速0.8 mL/min,柱温30 ℃,采用双波长检测。结果 生半夏共有10个共有峰,通过对照品比对指认了其中5个共有峰;伪品半夏中同时含有8种有效成分;随机选取部分生半夏与伪品半夏样品建立叠加指纹图谱得出:虎掌南星于34~38 min出现2个特征峰;姜水半夏在32~34 min出现2个特征峰;而水半夏出峰整体峰高较低,峰面积偏小。结论 建立的指纹图谱特征性强,能明显区分半夏与其伪品水半夏及虎掌南星,可用于半夏的鉴别和质量控制。Abstract: OBJECTIVE To develop a high performance liquid chromatography (HPLC) method for the fingerprint analysis of Pinellia ternata and its authenticity identification. METHODS HPLC analysis was performed on a SB-Aq column(4.6 mm×250 mm, 5 μm) with the mixture of 0.2% ammonium dihydrogen phosphate solution ( pH=3.0) (A) and methanol (B) as mobile phase in gradient mode. The column temperature was set at 30 ℃ and dual wavelength detection was used. RESULTS The HPLC fingerprint of Pinellia ternata,showing 10 characteristic peaks,was established from raw Pinellia ternata, and 5 were identified by standard comparison, and 8 common constituents were identified in fake Pinellia ternata samples. An overlay fingerprint were established with samples randomly selected: From 34 to 38 min,there were two characteristic peaks in the Pedate Rhizome Pinellia, and two appeared at 32 to 34 min in the Rhizoma Typhonii Flagelliformis prepared with ginger juice,while the peak height in the raw Typhonii flagelliformis Rhizoma was lower and the peak area was smaller. CONCLUSION The fingerprint of Pinellia ternata is significantly different from its confused herbs, which can be applied to the identification and quality control of Pinellia ternata.
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Key words:
- Pinellia ternata<\i> /
- fingerprints /
- counterfeit /
- identification /
- quality control
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[1] 牛倩,郭伟娜,黄和平. 道地中药颖半夏组培快繁优化研究[J]. 中药材,2017.40(8):1777- 1779. [2] 刘洋. 半夏组培苗的生根与移栽技术研究[J]. 北方园艺,2012(17):161-162. [3] 田红艳. 中药半夏的炮制方法与临床应用研究[J]. 世界最新医学信息文摘,2019,19(25): 150-151. [4] 左军,牟景光,胡晓阳. 半夏化学成分及现代药理作用研究进展[J]. 辽宁中医药大学学报,2019,21(9):26-29. [5] 武峰,秦志丰,李勇进,等. 半夏化学成分抗肿瘤研究进展[J]. 中华中医药学刊,2013,32(2): 270-272. [6] JI X, HUANG B, WANG G, et al. The ethnobotanical,phytochemical and pharmacological profile of the genus Pinellia[J]. Fitoterapia, 2014, 93:1-17. [7] 吴艳. 半夏与其易混品天南星的鉴别[J]. 时珍国医国药,2003,14(1):33-34. [8] 王彩琴,杜娟,王一敏,等. 甘肃半夏资源及研究现况[J]. 西部中医药,2015(3):22-24. [9] LI F, WU H, SUN LL, et al. Quantitative analysis of multi-components by single marker and fingerprint analysis of achyranthes bidentata blume[J]. J Chromatograph Sci, 2018,56(12):1-6. [10] LI G, YU F, WANG Y, et al. Comparison of the chromatographic fingerprint, multi-component quantitation and antioxidant activity of Salvia miltiorrhiza<\i>Bge. between sweating and non-sweating[J]. Biomed Chromatogr, 2018,32(6): e4203. [11] KAORI SP, GALO MG, GOMES ADRIANO DE A, et al. Mixture design PARAFAC HPLC-DAD metabolomic fingerprints of fractionated organic and basic extracts from erythrina speciosa andrews leaves[J]. Chromatographia, 2018,81(8):1189-1200. [12] 国家药典委员会.中华人民共和国药典:一部[S]. 北京:中国医药科技出版社,2015:附录119-121. [13] 肖琦,阳文武,张德伟,等. 半夏总生物碱含量影响因素及药理作用研究进展[J]. 中国药业, 2016,25(3):123-126. [14] 聂黎行,康帅,张南平,等. 高分离度快速液相色谱-三重串联四极杆质谱快速检查半夏和虎掌南星中水麦冬酸[J]. 药物评价研究,2018,41(9):1640-1643.
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