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槐耳颗粒逆转肝癌细胞对索拉非尼耐药的初步研究

张正光 刘冰 梁炜锋 沈存思

张正光, 刘冰, 梁炜锋, 沈存思. 槐耳颗粒逆转肝癌细胞对索拉非尼耐药的初步研究[J]. 南京中医药大学学报, 2020, 36(1): 83-87.
引用本文: 张正光, 刘冰, 梁炜锋, 沈存思. 槐耳颗粒逆转肝癌细胞对索拉非尼耐药的初步研究[J]. 南京中医药大学学报, 2020, 36(1): 83-87.
ZHANGZheng-guang, LIUBing, LIANGWei-feng, SHENCun-si. Preliminary Study on Reversal of Sorafenib Resistance of Hepatocellular Carcinoma Cells by Huaier Granule[J]. Journal of Nanjing University of traditional Chinese Medicine, 2020, 36(1): 83-87.
Citation: ZHANGZheng-guang, LIUBing, LIANGWei-feng, SHENCun-si. Preliminary Study on Reversal of Sorafenib Resistance of Hepatocellular Carcinoma Cells by Huaier Granule[J]. Journal of Nanjing University of traditional Chinese Medicine, 2020, 36(1): 83-87.

槐耳颗粒逆转肝癌细胞对索拉非尼耐药的初步研究

Preliminary Study on Reversal of Sorafenib Resistance of Hepatocellular Carcinoma Cells by Huaier Granule

  • 摘要: 目的 初步探讨槐耳颗粒对索拉非尼(Sorafenib)耐药肝癌细胞BEL-7402/S的作用及其相关机制。方法 CCK-8法测定槐耳颗粒对BEL-7402/S细胞的增殖-毒性作用以及对Sorafenib的耐药逆转倍数;qPCR及Western blot法检测槐耳颗粒对BEL-7402/S细胞的缺氧诱导因子-1α(HIF-1α)及血管内皮生长因子(VEGF) mRNA和蛋白表达水平的影响。结果 槐耳颗粒能够抑制BEL-7402/S细胞的活性;在无显著细胞毒性的剂量下,槐耳颗粒能够部分逆转BEL-7402/S细胞对Sorafenib的耐药,其耐药逆转倍数为2.08;qPCR结果显示槐耳颗粒可以下调BEL-7402/S细胞中HIF-1α的mRNA水平,但对VEGF的mRNA水平无显著性影响;Western blot结果显示槐耳颗粒可以降低HIF-1α、VEGF的蛋白表达水平。结论 槐耳颗粒可能具有部分逆转BEL-7402/S细胞对Sorafenib耐药的作用,其机制可能与降低HIF-1α以及VEGF的表达水平有关。

     

  • [1] BRAY F, FERLAY J, SOERJOMATARAM I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018,68(6):394-424.
    [2] KING J, PALMER DH, JOHNSON P, et al. Sorafenib for the treatment of advanced hepatocellular cancer-a UK audit[J]. Clin Oncol, 2017,29(4):256-262.
    [3] APOSTOLIDIS L, PFEIFFENBERGER J, GOTTHARDT D, et al. Survival of hepatocellular carcinoma patients treated with sorafenib beyond progression[J]. Gastrointest Tumors, 2018,5(1/2):38-46.
    [4] QIU J, LIU D, YAN Z, et al. Therapeutic effect and adverse reaction of sorafenib in the treatment of advanced renal cancer[J]. Oncol Lett, 2019,17(2):1547-1550.
    [5] KUCZYNSKI EA, LEE CR, MAN S, et al. Effects of sorafenib dose on acquired reversible resistance and toxicity in hepatocellular Carcinoma[J]. Cancer Res, 2015,75(12):2510-2519.
    [6] ZHAO GS, LIU Y, ZHANG Q, et al. Transarterial chemoembolization combined with Huaier granule for the treatment of primary hepatic carcinoma[J]. Medicine, 2017,96(29):e7589.
    [7] FU Z, MA K, DONG B, et al. The synergistic antitumor effect of Huaier combined with 5-Florouracil in human cholangiocarcinoma cells[J]. BMC Complement Altern Med, 2019,19(1):203-214.
    [8] 唐亦非, 朱晓骏, 黄凌鹰, 等. 槐耳颗粒联合索拉非尼治疗晚期肝癌的临床研究[J]. 现代药物与临床, 2018,33(7):1732-1735.
    [9] 李戎, 谢莎, 张莉, 等. 槐耳颗粒逆转人乳腺癌细胞MCF-7耐药的初步机制[J]. 中国实用医药, 2009,4(17):1-3.
    [10] 程万宏, 王艳俊, 应朝辉, 等. 槐耳清膏和TOR逆转人肺腺癌细胞顺铂耐药性实验研究[J]. 辽宁中医药大学学报, 2017,19(2):23-25.
    [11] 刘念. 槐耳清膏体外逆转人肝癌细胞HepG2/ADM多药耐药性[D].合肥:安徽医科大学, 2007.
    [12] WANG X, ZHANG N, HUO Q, et al. Huaier aqueous extract suppresses human breast cancer cell proliferation through inhibition of estrogen receptor alpha signaling[J]. Int J Oncol, 2013,43(1):321-328.
    [13] ZHANG T, WANG KE, ZHANG J, et al. Huaier aqueous extract inhibits colorectal cancer stem cell growth partially via downregulation of the Wnt/β-catenin pathway[J]. Oncol Lett, 2013,5(4):1171-1176.
    [14] SHAN L, LI Y, JIANG H, et al. Huaier restrains proliferative and migratory potential of hepatocellular carcinoma cells partially through decreased yes-associated protein 1[J]. J Cancer, 2017,8(19):4087-4097.
    [15] WU T, CHEN W, LIU S, et al. Huaier suppresses proliferation and induces apoptosis in human pulmonary cancer cells via upregulation of miR-26b-5p[J]. FEBS Lett, 2014,588(12):2107-2114.
    [16] ZHANG F, ZHANG Z, LIU Z. Effects of huaier aqueous extract on proliferation and apoptosis in the melanoma cell line A875[J]. Acta Histochem, 2013,115(7):705-711.
    [17] XU Z, ZHENG G, WANG Y, et al. Aqueous huaier extract suppresses gastric cancer metastasis and epithelial to mesenchymal transition by targeting Twist[J]. J Cancer, 2017,8(18):3876-3886.
    [18] YAN X, LYU T, JIA N, et al. Huaier aqueous extract inhibits ovarian cancer cell motility via the AKT/GSK3beta/beta-catenin pathway[J]. PLoS ONE, 2013,8(5):e63731.
    [19] YANG A, FAN H, ZHAO Y, et al. Huaier aqueous extract inhibits proliferation and metastasis of tuberous sclerosis complex cell models through downregulation of JAK2/STAT3 and MAPK signaling pathways[J]. Oncol Rep, 2016,36(3):1491-1498.
    [20] HU Z, YANG A, FAN H, et al. Huaier aqueous extract sensitizes cells to rapamycin and cisplatin through activating mTOR signaling[J]. J Ethnopharmacol, 2016,186:143-150.
    [21] DING X, YANG Q, KONG X, et al. Radiosensitization effect of Huaier on breast cancer cells[J]. Oncol Rep, 2016,35(5):2843-2850.
    [22] CHEN Q, SHU C, LAURENCE AD, et al. Effect of Huaier granule on recurrence after curative resection of HCC: a multicentre, randomised clinical trial[J]. Gut, 2018,67(11):2006-2016.
    [23] TAO Y, SHAN L, XU X, et al. Huaier augmented the chemotherapeutic sensitivity of oxaliplatin via downregulation of YAP in hepatocellular carcinoma[J]. J Cancer, 2018,9(21):3962-3970.
    [24] SONG Z, LIU T, CHEN J, et al. HIF-1α-induced RIT1 promotes liver cancer growth and metastasis and its deficiency increases sensitivity to sorafenib[J]. Cancer Lett, 2019,460:96-107.
    [25] QIU Y, SHAN W, YANG Y, et al. Reversal of sorafenib resistance in hepatocellular carcinoma: epigenetically regulated disruption of 14-3-3η/hypoxia-inducible factor-1α[J]. Cell Death Discov, 2019,5(1):120-130.
    [26] MNDEZ-BLANCO C, FONDEVILA F, GARCA-PALOMO A, et al. Sorafenib resistance in hepatocarcinoma: role of hypoxia-inducible factors[J]. Exp Mol Med, 2018,50(10):134-142.
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  • 刊出日期:  2020-01-10

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