熄风化湿方对腹泻型肠易激综合征大鼠内脏高敏感性的影响
Effect of Xifeng Huashi Recipe on Visceral Hypersensitivity in Rats with Diarrhea Predominant Irritable Bowel Syndrome
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摘要: 目的 探讨熄风化湿方(XFHS)对腹泻型肠易激综合征(IBS-D)大鼠内脏高敏感性及SCF-Kit系统的影响。方法 Wistar大鼠随机分为正常对照组,模型组,XFHS低、中、高剂量组。采用“母婴分离+乙酸灌肠”法建立IBS-D大鼠模型,XFHS低、中、高剂量组分别给予低、中、高剂量(10.9、21.8、43.6 g/kg)的XFHS方灌胃,正常对照组和模型组给予等容积的蒸馏水灌胃,测定腹壁撤退反射评分(AWR),用Western blot和qPCR法测定结肠组织SCF、c-Kit蛋白和mRNA含量。结果 模型组大鼠AWR评分,SCF、c-Kit蛋白和mRNA含量均显著高于正常对照组(P<0.05)。而XFHS组大鼠AWR评分、SCF、c-Kit蛋白和mRNA含量均低于模型组(P<0.05)。结论 XFHS方可改善IBS-D大鼠的内脏高敏感性,可能与降低SCF-Kit系统的过度表达有关。Abstract: OBJECTIVE To investigate the effect of Xifeng Huashi prescription (XFHS) on visceral hypersensitivity and SCF-Kit system in rats with diarrhea predominant irritable bowel syndrome (IBS-D). METHODS Rats were randomly divided into control group,model group, XFHS low, moderate and high dosage groups. The model was induced by separation of breast milk and coloclysis of acetic acid. High, moderate and low dosage XFHS group rats were given by gavage with different concentration of XFHS decoction,respectively. Equal saline was administrated in the normal group and model group. The abdominal withdrawal reflex was recorded, and the levels of SCF and c-Kit in the colonic tissue were detected by Western blot and qPCR. RESULTS Comparing with normal group, the grades of AWR and the levels of gastric SCF and c-Kit expression in the model group were significantly lower (P<0.05). The grades of AWR and the levels of gastric SCF and c-Kit expression in XFHS groups were significantly lower than those in the model group (P<0.05). CONCLUSION XFHS can reduce visceral hypersensitivity by inhibiting the expression of SCF-Kit system.
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