留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

香参壳方对人胃癌细胞上皮间质转化及转移的影响

吕兴 吕高虹 叶冠

吕兴, 吕高虹, 叶冠. 香参壳方对人胃癌细胞上皮间质转化及转移的影响[J]. 南京中医药大学学报, 2019, 35(6): 687-690.
引用本文: 吕兴, 吕高虹, 叶冠. 香参壳方对人胃癌细胞上皮间质转化及转移的影响[J]. 南京中医药大学学报, 2019, 35(6): 687-690.
LYUXing, LYUGao-hong, YEGuan. Protective Effect of Xiangshenke Formula on the Epithelial Mesenchymal Transformation and Metastasis of Human Gastric Carcinoma Cells[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(6): 687-690.
Citation: LYUXing, LYUGao-hong, YEGuan. Protective Effect of Xiangshenke Formula on the Epithelial Mesenchymal Transformation and Metastasis of Human Gastric Carcinoma Cells[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(6): 687-690.

香参壳方对人胃癌细胞上皮间质转化及转移的影响

Protective Effect of Xiangshenke Formula on the Epithelial Mesenchymal Transformation and Metastasis of Human Gastric Carcinoma Cells

  • 摘要: 目的 考察香参壳方调节人胃癌细胞SGC-7901上皮间质转化(EMT)及转移的作用机制。方法 体外培养SGC-7901细胞,随后加入不同浓度的香参壳方含药血清进行干预。划痕实验及Transwell小室法观察SGC-7901细胞的迁移与侵袭能力,MTT法考察SGC-7901细胞的增殖能力,Western blot法检测细胞中N-cadherin、Vimentin、E-cadherin、p-GSK3β/GSK3β、β-Catenin表达水平。结果 香参壳方可有效抑制SGC-7901细胞的迁移及侵袭能力,抑制其增殖,同时下调细胞中N-cadherin、Vimentin、p-GSK3β/GSK3β及β-Catenin的表达,上调E-cadherin的表达。结论 香参壳方主要通过介导GSK3β/β-Catenin信号通路抑制胃癌细胞的EMT,降低弱细胞迁移与侵袭能力,从而发挥其胃癌术后的辅助治疗作用。

     

  • [1] VAN CUTSEM E, SAGAERT X, TOPAL B, et al. Gastric cancer[J]. Lancet, 2016, 388(10060): 2654-2664.
    [2] FIDLER IJ. The pathogenesis of cancer metastasis: The "seed and soil" hypothesis revisited[J]. Nat Rev Cancer, 2003, 3(6): 453-458.
    [3] YONEMURA Y , BANDOU E , KAWAMURA T , et al. Quantitative prognostic indicators of peritoneal dissemination of gastric cancer[J]. Europ J Surgic Oncol, 2006, 32(6):602-606.
    [4] LIU J, WANG S, ZHANG Y, et al. Traditional Chinese medicine and cancer: History, present situation, and development[J]. Thoracic Cancer, 2015, 6(5): 561-569.
    [5] 徐淑云, 卞如濂, 陈修. 药理实验方法学[M].3版. 北京: 人民卫生出版社, 2005: 203.
    [6] 潘卫松, 刘美凤, 石钺, 等. 血清药理学、血清化学和中药药代动力学[J]. 世界科学技术—中医药现代化, 2002, 4(3):53-56.
    [7] 李仪奎. 中药血清药理学实验方法的若干问题[J]. 中药新药与临床药理, 1999,10(2):95-98.
    [8] 鄂征. 组织培养和分子细胞学技术[M]. 北京:北京出版社, 2001:207-214.
    [9] 王力倩, 李仪奎, 符胜光, 等. 血清药理学方法研究探索[J]. 中药药理与临床, 1997,10(3):29-31.
    [10] ZHANG F, ZHANG X, LI M, et al. mTOR complex component Rictor interacts with PKC zeta and regulates cancer cell metastasis[J]. Cancer Res, 2010, 70(22):9360-9370.
    [11] 王晓君, 吴乾. 中医健脾祛瘀法治疗胃癌的应用与疗效评价[J]. 中国药物与临床, 2018,18(1): 117-118.
    [12] FELIPE LJ, NOFECH-MOZES S, BAYANI J, et al. EMT in breast carcinoma:A review[J]. J Clin Med, 2016, 5(7): 65-79.
    [13] MOHAMMED MK, SHAO C, WANG J, et al. Wnt/β-catenin signaling plays an ever-expanding role in stem cell self-renewal, tumorigenesis and cancer chemoresistance[J]. Gene Dis, 2016, 3(1):11-40.
    [14] FASSAN M, SIMBOLO M, BRIA E, et al. High-throughput mutation profiling identifies novel molecular dysregulation in high-grade intraepithelial neoplasia and early gastric cancers[J]. Gastric Cancer, 2014, 17(3): 442-449.
    [15] ANASTAS JN, MOON RT. WNT signalling pathways as therapeutic targets in cancer[J]. Nat Rev Cancer, 2013, 13(1): 11-26.
    [16] WU J, ZHANG HT, XU C, et al. TIPE2 functions as a metastasis suppressor via negatively regulating β-catenin through activating GSK3β in gastriccancer[J]. Int J Oncol, 2016, 48(1):199-206.
  • 加载中
计量
  • 文章访问数:  535
  • HTML全文浏览量:  11
  • PDF下载量:  516
  • 被引次数: 0
出版历程
  • 刊出日期:  2019-11-10

目录

    /

    返回文章
    返回