香参壳方对人胃癌细胞上皮间质转化及转移的影响
Protective Effect of Xiangshenke Formula on the Epithelial Mesenchymal Transformation and Metastasis of Human Gastric Carcinoma Cells
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摘要: 目的 考察香参壳方调节人胃癌细胞SGC-7901上皮间质转化(EMT)及转移的作用机制。方法 体外培养SGC-7901细胞,随后加入不同浓度的香参壳方含药血清进行干预。划痕实验及Transwell小室法观察SGC-7901细胞的迁移与侵袭能力,MTT法考察SGC-7901细胞的增殖能力,Western blot法检测细胞中N-cadherin、Vimentin、E-cadherin、p-GSK3β/GSK3β、β-Catenin表达水平。结果 香参壳方可有效抑制SGC-7901细胞的迁移及侵袭能力,抑制其增殖,同时下调细胞中N-cadherin、Vimentin、p-GSK3β/GSK3β及β-Catenin的表达,上调E-cadherin的表达。结论 香参壳方主要通过介导GSK3β/β-Catenin信号通路抑制胃癌细胞的EMT,降低弱细胞迁移与侵袭能力,从而发挥其胃癌术后的辅助治疗作用。
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关键词:
- 香参壳方 /
- 人胃癌细胞 /
- SGC-7901 /
- 上皮间质转化 /
- GSK3β/β-Catenin信号通路
Abstract: OBJECTIVE To investigate the mechanism of Xiangshenke formula in regulating the proliferation, migration and EMT of human gastric carcinoma cells SGC-7901. METHODS SGC-7901 cells were cultured in vitro, and then different concentrations of Xiangshenke formula containing serum were added into the cells. Wound healing test and transwell chamber assay were used to observe the SGC-7901 cell migration and invasion. MTT assay was used to investigate the SGC-7901 cell proliferation. Western blot was used to detect the expression levels of N-cadherin, Vimentin, E-cadherin, p-GSK3β/GSK3β and β-Catenin. RESULTS Xiangshenke formula effectively inhibited the migration and invasion of SGC-7901 cells, suppressed the cell proliferation, down-regulated the expression of N-cadherin, Vimentin, p-GSK3β/GSK3β and β-Catenin, and up-regulated the expression of E-cadherin. CONCLUSION Xiangshenke formula can inhibit the SGC-7901 cell epithelial-mesenchymal transition by regulating the GSK3β/β-Catenin signaling pathway, and suppress their migrtaion and invasion ability. It plays an important role in adjuvant therapy after gastric cancer surgery. -
[1] VAN CUTSEM E, SAGAERT X, TOPAL B, et al. Gastric cancer[J]. Lancet, 2016, 388(10060): 2654-2664. [2] FIDLER IJ. The pathogenesis of cancer metastasis: The "seed and soil" hypothesis revisited[J]. Nat Rev Cancer, 2003, 3(6): 453-458. [3] YONEMURA Y , BANDOU E , KAWAMURA T , et al. Quantitative prognostic indicators of peritoneal dissemination of gastric cancer[J]. Europ J Surgic Oncol, 2006, 32(6):602-606. [4] LIU J, WANG S, ZHANG Y, et al. Traditional Chinese medicine and cancer: History, present situation, and development[J]. Thoracic Cancer, 2015, 6(5): 561-569. [5] 徐淑云, 卞如濂, 陈修. 药理实验方法学[M].3版. 北京: 人民卫生出版社, 2005: 203. [6] 潘卫松, 刘美凤, 石钺, 等. 血清药理学、血清化学和中药药代动力学[J]. 世界科学技术—中医药现代化, 2002, 4(3):53-56. [7] 李仪奎. 中药血清药理学实验方法的若干问题[J]. 中药新药与临床药理, 1999,10(2):95-98. [8] 鄂征. 组织培养和分子细胞学技术[M]. 北京:北京出版社, 2001:207-214. [9] 王力倩, 李仪奎, 符胜光, 等. 血清药理学方法研究探索[J]. 中药药理与临床, 1997,10(3):29-31. [10] ZHANG F, ZHANG X, LI M, et al. mTOR complex component Rictor interacts with PKC zeta and regulates cancer cell metastasis[J]. Cancer Res, 2010, 70(22):9360-9370. [11] 王晓君, 吴乾. 中医健脾祛瘀法治疗胃癌的应用与疗效评价[J]. 中国药物与临床, 2018,18(1): 117-118. [12] FELIPE LJ, NOFECH-MOZES S, BAYANI J, et al. EMT in breast carcinoma:A review[J]. J Clin Med, 2016, 5(7): 65-79. [13] MOHAMMED MK, SHAO C, WANG J, et al. Wnt/β-catenin signaling plays an ever-expanding role in stem cell self-renewal, tumorigenesis and cancer chemoresistance[J]. Gene Dis, 2016, 3(1):11-40. [14] FASSAN M, SIMBOLO M, BRIA E, et al. High-throughput mutation profiling identifies novel molecular dysregulation in high-grade intraepithelial neoplasia and early gastric cancers[J]. Gastric Cancer, 2014, 17(3): 442-449. [15] ANASTAS JN, MOON RT. WNT signalling pathways as therapeutic targets in cancer[J]. Nat Rev Cancer, 2013, 13(1): 11-26. [16] WU J, ZHANG HT, XU C, et al. TIPE2 functions as a metastasis suppressor via negatively regulating β-catenin through activating GSK3β in gastriccancer[J]. Int J Oncol, 2016, 48(1):199-206.
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