栀子苷对Treg细胞分化和功能的影响及其机制研究

卞勇; 章雯; 时乐; 喻斌; 俞云; 崔伟伟

栀子苷对Treg细胞分化和功能的影响及其机制研究

卞勇¹,
章雯²,
时乐³,
喻斌³,
俞云³,
崔伟伟⁴

1.
南京中医药大学实验动物中心，江苏南京 210023
2.
南京医科大学口腔疾病研究江苏省重点实验室，南京医科大学附属口腔医院，江苏南京 210029
3.
南京中医药大学药学院，江苏南京 210023
4.
南京中医药大学附属中西医结合医院，江苏南京 210028

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出版历程
- 刊出日期: 2019-11-10

Effect of Jasminoidin on Differentiation and Function of Treg Cells and Its Mechanism

BIANYong¹,
ZHANGWen²,
SHILe³,
YUBin³,
YUYun³,
CUIWei-wei⁴

1.
Laboratory Animal Center, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
Jiangsu Key Laboratory of Oral Diseases Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, China
3.
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China
4.
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, China

摘要

摘要: 目的考察栀子苷对初始T细胞（Nave T）向Treg定向分化的影响和机制。方法通过磁珠分选小鼠脾脏CD4⁺CD62L⁺T，诱导其向Treg分化，单独使用栀子苷以及与ERK通路阻断剂、JNK阻断剂合用，流式细胞术检测Treg细胞比例，Western blot法检测转录因子Foxp3、ERK、p-ERK、JNK、p-JNK等表达，ELISA法检测细胞上清IL-10含量。结果与Control组比较，栀子苷组Treg细胞比例，Foxp3、p-ERK、p-JNK表达，IL-10含量显著增高（P<0.05~0.01）；单纯诱导的基础上先加入ERK通路阻断剂再加入栀子苷，其Treg细胞比例、Foxp3表达水平、IL-10含量显著低于栀子苷组（P<0.05~0.01），但高于Control组（P<0.01）；单纯诱导的基础上先加入JNK阻断剂再加入栀子苷，其Treg细胞比例、Foxp3表达水平、IL-10含量显著低于栀子苷组（P<0.05~0.01），但高于Control组（P<0.01）。结论栀子苷可以显著促进Nave T向Treg定向分化，同时增强其功能，ERK、JNK可能和其发挥作用有一定关系。
- 栀子苷 /
- Treg /
- ERK /
- JNK
Abstract: OBJECTIVE To investigate the effect and mechanism of Jasminoidin on the directional differentiation of Nave T into Treg. METHODS CD4⁺CD62L⁺T was isolated from the spleen of mice by magnetic beads and induced to differentiate into Treg. Jasminoidin was used alone， and combined with ERK pathway blocker and JNK blocker. FCM was used to detect the proportion of Treg cells， and Western blot was used to detect the transcription factors of Foxp3， ERK， p-ERK，JNK and p-JNK. The expression of IL-10 were detected by ELISA method. RESULTS The percentage of Treg cells， the expression of Foxp3， p-ERK， p-JNK and the content of IL-10 in Jasminoidin group were significantly higher than those in control group when Jasminoidin was used on the basis of induction alone. On the basis of induction alone， ERK pathway blockers were added before Jasminoidin. The percentage of Treg cells， the expression of Foxp3 and the content of IL-10 were significantly lower than those of Jasminoidin group， but higher than those of control group. On the basis of induction alone， JNK blockers were added before Jasminoidin. The proportion of Treg cells， the expression of Foxp3， and the content of IL-10 were significantly lower than those of Jasminoidin group， but higher than those of control group. CONCLUSION Jasminoidin can promote the directional differentiation of Nave T into Treg and enhance its function， which may be associated with ERK and JNK.
- Jasminoidin /
- Treg /
- ERK /
- JNK

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参考文献(13)

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