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垫状卷柏提取物诱导凋亡抗肿瘤作用研究

樊炼 吴永平 瞿慧 吴信华 曹园

樊炼, 吴永平, 瞿慧, 吴信华, 曹园. 垫状卷柏提取物诱导凋亡抗肿瘤作用研究[J]. 南京中医药大学学报, 2019, 35(6): 664-670.
引用本文: 樊炼, 吴永平, 瞿慧, 吴信华, 曹园. 垫状卷柏提取物诱导凋亡抗肿瘤作用研究[J]. 南京中医药大学学报, 2019, 35(6): 664-670.
FANLian, WUYong-ping, QUHui, WUXin-hua, CAOYuan. Selaginella Pulvinata Extract Exerts Antitumor Efficacy in H22 Tumor-Bearing Mice via Induction of Apoptosis[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(6): 664-670.
Citation: FANLian, WUYong-ping, QUHui, WUXin-hua, CAOYuan. Selaginella Pulvinata<\i> Extract Exerts Antitumor Efficacy in H22 Tumor-Bearing Mice via Induction of Apoptosis[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(6): 664-670.

垫状卷柏提取物诱导凋亡抗肿瘤作用研究

Selaginella Pulvinata<\i> Extract Exerts Antitumor Efficacy in H22 Tumor-Bearing Mice via Induction of Apoptosis

  • 摘要: 目的 研究垫状卷柏提取物抗肿瘤作用及相关机制。方法 采用皮下接种H22肝癌细胞株构建小鼠H22肝癌移植瘤模型,随机分为对照组(生理盐水)、5-FU组(30 mg/kg)、垫状卷柏提取物(SP)高、低剂量组(189、63 mg/kg),连续灌胃15 d。观察小鼠肿瘤生长情况,计算抑瘤率及肝、脾、胸腺指数;TUNEL法观察肿瘤组织细胞凋亡,Western blot测定凋亡相关蛋白的表达,qPCR检测Bax mRNA和Bcl-2 mRNA。结果 与对照组比较,SP高、低剂量组小鼠体质量及肝、脾、胸腺指数均无显著差异;SP高、低剂量组小鼠的肿瘤质量均显著降低,呈现较高抑瘤率(P<0.01),且高剂量组与5-FU组相近;经TUNEL染色法观察,可见明显凋亡形态改变。qPCR检测表明,SP显著上调Bax mRNA的表达,下调Bcl-2 mRNA的表达。Western blot结果显示SP处理后,Bax、cytochrome c及cleaved caspase-3,caspase-8和caspase-9的表达显著上调(P<0.01),Bcl-2表达明显减弱,呈剂量依赖性。结论 SP有效抑制H22小鼠体内肿瘤生长,其机制与激活caspase诱导凋亡相关。

     

  • [1] SIEGEL R, MILLER K, JEMAL A. Cancer statistics [J]. CA Cancer J Clin, 2017, 67(1): 7-30.
    [2] VENOOK A, PAPANDREOU C, FURUSE J, et al. The Incidence and epidemiology of hepatocellular carcinoma: A global and regional perspective [J]. Oncologist, 2010, 15(S4): 5-13.
    [3] Editorial Board of "Zhong Hua Ben Cao", State Administration of Traditional Chinese Medicine of the People's Republic of China. Zhong Hua Ben Cao (Vol 4) [M]. Shanghai: Shanghai Scientific and Technical Publishers, 1999: 387.
    [4] CAO Y, CHEN JJ, TAN NH, et al. Antimicrobial selaginellin derivatives from Selaginella pulvinata<\i> [J]. Bioorg Med Chem Lett, 2010, 20(8): 2456-2460.
    [5] CAO Y, CHEN JJ, TAN NH, et al. Structure determination of selaginellins G and H from Selaginella pulvinata<\i> by NMR spectroscopy [J]. Magn Reson Chem, 2010, 48(8): 656-659.
    [6] CAO Y, YAO Y, HUANG XJ, et al. Four new selaginellin derivatives from Selaginella pulvinata<\i>: Mechanism of racemization process in selaginellins with quinone methide [J]. Tetrahedron, 2015, 71(10): 1581-1587.
    [7] CAO Y, ZHAO M, ZHU Y, et al. Diselaginellin B, an unusual dimeric molecule from Selaginella pulvinata<\i>, inhibited metastasis and induced apoptosis of SMMC-7721 human hepatocellular carcinoma cells [J]. J Nat Prod, 2017, 80(12): 3151-3158.
    [8] CAO Y, WU YP, ZHOU XW, et al. Simultaneous determination of selaginellins and biflavones in Selaginella tamariscina<\i> and Selaginella pulvinata<\i> by HPLC-DAD [J]. China J Chin Mater Med, 2012, 37(9): 1254-1258.
    [9] LEE IS, NISHIKAWA A, FURUKAWA F, et al. Effects of Selaginella tamariscina<\i> on in vitro<\i> tumor cell growth, p53 expression, G1 arrest and in vivo<\i> gastric cell proliferation [J]. Cancer Lett, 1999, 144(1): 93-99.
    [10] YANG JS, LIN CW, HSIN CH, et al. Selaginella tamariscina<\i> attenuates metastasis via Akt pathways in oral cancer cells [J]. PLoS ONE, 2013, 8(6): e68035.
    [11] YANG SF, CHU SC, LIU SJ, et al. Antimetastatic activities of Selaginella tamariscina<\i> (Beauv.) on lung cancer cells in vitro <\i>and in vivo<\i> [J]. J Ethnopharmacol, 2007, 110(3): 483-489.
    [12] HSIN CH, WU BC, CHUANG CY, et al. Selaginella tamariscina<\i> extract suppresses TPA-induced invasion and metastasis through inhibition of MMP-9 in human nasopharyngeal carcinoma HONE-1 cells [J]. BMC Complement Altern Med, 2013, 13: 234.
    [13] LEE S, KIM H, KANG JW, et al. The biflavonoid amentoflavone induces apoptosis via suppressing E7 expression, cell cycle arrest at sub-G1 phase, and mitochondria-emanated intrinsic pathways in human cervical cancer cells [J]. J Med Food, 2011, 14(14): 808-816.
    [14] PEI JS, LIU CC, HSU YN, et al. Amentoflavone induces cell-cycle arrest and apoptosis in MCF-7 human breast cancer cells via mitochondria-dependent pathway [J]. In Vivo, 2012, 26(6): 963-970.
    [15] GURUVAYOORAPPAN C,KUTTAN G. Amentoflavone inhibits experimental tumor metastasis through a regulatory mechanism involving MMP-2, MMP-9, prolyl hydroxylase, lysyl oxidase, VEGF, ERK-1, ERK-2, STAT-1, nm23 and cytokines in lung tissues of C57BL/6 mice [J]. Immunopharmacol Immunotoxicol, 2008, 30(4): 711-727.
    [16] GURUVAYOORAPPAN C, KUTTAN G. Inhibition of tumor specific angiogenesis by amentoflavone [J]. Biochemistry, 2008, 73(73): 209-218.
    [17] LEE JS, LEE MS, OH WK, et al. Fatty acid synthase inhibition by amentoflavone induces apoptosis and antiproliferation in human breast cancer cells [J]. Biol Pharm Bull, 2009, 32(8): 1427-1432.
    [18] LEE JS, SUL JY, PARK JB, et al. Fatty acid synthase inhibition by amentoflavone suppresses HER2/neu (erbB2) oncogene in SKBR3 human breast cancer cells [J]. Phytother Res, 2013, 27(5): 713-720.
    [19] KUHAJDA FP. Fatty acid synthase and cancer: New application of an old pathway [J]. Cancer Res, 2006, 66(12): 5977-5980.
    [20] PANDEY PR, LIU W, XING F, et al. Anti-cancer drugs targeting fatty acid synthase (FAS) [J]. Recent Pat Anticancer Drug Discov, 2012, 7(2): 185-197.
    [21] BOSISIO E, SAPONARA R. Inhibition of cAMP-Phosphodiesterase by biflavones of Ginkgo biloba <\i>in rat adipose tissue [J]. J Nat Prod, 1998, 61(11): 1386-1387.
    [22] SHIM SY, LEE S, LEE M. Biflavonoids isolated from Selaginella tamariscina<\i> and their anti-inflammatory activities via ERK 1/2 signaling [J]. Molecules, 2018, 23: 926.
    [23] SILVA GL, CHAI H, GUPTA MP, et al. Cytotoxic biflavonoids from Selaginella willdenowii<\i> [J]. Phytochemistry, 1995, 40(1): 129-134.
    [24] ZHANG GG, JING Y, ZHANG HM, et al. Isolation and cytotoxic activity of selaginellin derivatives and biflavonoids from Selaginella tamariscina<\i> [J]. Planta Med, 2012, 78(4): 390-392.
    [25] YANG C, SHAO YT, LI K, et al. Bioactive selaginellins from Selaginella tamariscina <\i>(Beauv.) Spring [J]. Beilstein J Org Chem, 2012, 8(1): 1884-1889.
    [26] HANAHAN D, WEINBERG RA. Hallmarks of cancer: the next generation [J]. Cell, 2011, 144(5): 646-674.
    [27] PORTT L, NORMAN G, CLAPP C, et al. Anti-apoptosis and cell survival: A review [J]. Biochim Biophys Acta, 2011, 1813(1): 238-259.
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  • 刊出日期:  2019-11-10

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