Abstract: Traditional Chinese medicine (TCM) was based on the theory of yin-yang， whose medical knowledge and philosophical thought remained enduring after thousands of years. This paper had a deep exploration of the connotation and evolution of female reproductive physiology. With analyzing the role that yin-yang played in menstrual regulation， it was hold that interdependence between yin-yang was a state of relative equilibrium and a premise of periodic steady state. Imbalance of yin-yang promoted the evolution of the cycle. The transformation of yin-yang determined menstrual cycle rhythm. Eight Diagrams theory facilitated multi-level yin-yang balance and contained periodic trends of yin-yang circular motion. Twelve divinatory symbols represented that the growth and decline of natural climate was consistent with those of menstrual cycle， which also had close relation with female reproductive and menstrual cycle rhythm. Based on theories in TCM classics， this paper put forward more precise methods to regulate menstruation with TCM， which had promoted academic value of TCM gynecology， followed the changes of spectrum of disease and provided a good foundation of preventing gynecological diseases.