当归四逆汤对奥沙利铂神经毒性大鼠背根神经节TRPs通道的影响

丁蓉; 汪悦; 卢悟广; 魏国利; 顾展丞; 霍介格

当归四逆汤对奥沙利铂神经毒性大鼠背根神经节TRPs通道的影响

1.
南京中医药大学附属中西医结合医院，江苏南京 210028
2.
南京中医药大学第一临床医学院，江苏南京 210023
3.
海门市中医院骨科，江苏海门 226100

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出版历程
- 刊出日期: 2017-01-10

The Effect of Danggui Sini Decoction on TRPs Channel in the DRG of the OXIN Rats

1.
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine , Nanjing University of Chinese Medicine, Nanjing, 210028, China
2.
The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, 210023, China
3.
Department of Orthopedics, Haimen Hospital of Traditional Chinese Medicine, Haimen, 226100, China

摘要

摘要: 目的探讨当归四逆汤对奥沙利铂神经毒性大鼠背根神经节TRPs通道的影响。方法使用奥沙利铂腹腔注射造成慢性神经毒性大鼠模型，60只大鼠分空白组、模型组、及当归四逆汤高、中、低剂量组。通过大鼠疼痛行为学、机械疼痛阈值测定检测当归四逆汤对奥沙利铂神经毒性的缓解作用，并进一步通过RT-PCR、Western blot观察大鼠背根神经节TRPs（TRPV1、TRPA1、TRPM8）mRNA及蛋白表达水平变化。结果大鼠行为学测定显示，与空白组比较，模型组、当归四逆汤低、中剂量组机械疼痛阈值下降（P<0.05），而高剂量组未出现明显的下降（P＞0.05）。与空白组比较，其余各组大鼠背根神经节TRPA1、TRPM8、TRPV1蛋白表达量显著增加（P<0.01）。与模型组比较，当归四逆汤低剂量组可降低TRPM8蛋白表达（P<0.05），中剂量组可降低TRPA1（P<0.01）、TRPM8（P<0.01）、TRPV1（P<0.05）蛋白表达，高剂量组明显降低三者蛋白表达量（P<0.01）。与空白组比较，其余各组大鼠背根神经节TRPV1、TRPA1、TRPM8 mRNA表达显著增加（P<0.01），与模型组比较，当归四逆汤各组均可显著下调三者表达（P<0.01）。结论当归四逆汤可以预防奥沙利铂慢性神经毒性，减缓大鼠机械疼痛阈值下降程度，其机制可能与下调TRPs通道蛋白表达有关。
- 当归四逆汤 /
- 奥沙利铂 /
- 神经毒性 /
- TRPs通道
Abstract: OBJECTIVE To investigate the effect of Danggui Sini Decoction on TRPs channels in the dorsal root ganglia of the oxaliplatin-induced neurotoxic rats. METHODS A rat model of chronic neurotoxicity was induced by intraperitoneal injection of oxaliplatin. 60 rats were divided into blank group， model group， and Danggui Sini Decoction high， medium and low dose groups. The neurotoxicity of oxaliplatin was detected by rat pain behavior and mechanical pain threshold. The mRNA and protein expression levels change of TRPs (TRPV1， TRPA1， TRPM8) in rat dorsal root ganglion were further observed by RT-PCR and Western blot. RESULTS Compared with the blank group， the model group， the low- and medium-dose group of Danggui Sini Decoction showed a significant decrease in the mechanical pain threshold (P<0.05)， while the high-dose group did not show a significant decrease. Compared with the blank group， the expression of TRPA1， TRPM8 and TRPV1 protein in dorsal root ganglion of each group increased with oxaliplatin (P<0.01). Compared with the model group， the low dose group of Danggui Sini Decoction reduced TRPM8 protein expression (P<0.05)， and the middle dose group decreased TRPA1 (P<0.01)， TRPM8 (P<0.01)， TRPV1 (P<0.05) while high dose group can significantly reduce the expression of TRPs (TRPV1， TRPA1， TRPM8) (P<0.01). Compared with the blank group， the expression of TRPV1， TRPA1 and TRPM8 mRNA in the dorsal root ganglion of the rats in each group were significantly increased after application of oxaliplatin(P<0.01). Compared with the model group， each group could be significantly down-regulated after applying the Danggui decoction. Expression (P<0.01). CONCLUSION Danggui Sini Decoction can prevent the chronic neurotoxicity of oxaliplatin and slow down the decline of mechanical pain threshold in rats. The mechanism may be related to the down-regulation of TRP channel protein expression.
- Danggui Sini Decoction /
- oxaliplatin /
- neurotoxicity /
- TRPs channel

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参考文献(17)

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