电针不同穴组对功能性便秘大鼠结肠黏膜超微结构及AQP3、AQP8表达的影响

王渊; 王强; 刘思洋; 周锋; 牛文民; 刘智斌; 刘龙

电针不同穴组对功能性便秘大鼠结肠黏膜超微结构及AQP3、AQP8表达的影响

1.
陕西中医药大学针灸推拿学院，陕西咸阳 712046
2.
西安医学院临床医学院，陕西西安 710021
3.
陕西中医药大学附属医院，陕西咸阳 712000
4.
西安航天总医院中医针灸理疗科，陕西西安 710100

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出版历程
- 刊出日期: 2019-03-10

Influence on Ultrastructure of Colonic Mucosa in Colon， Expression of AQP3， AQP8 by Electroacupuncture at Different Acupoints Combination in Rats with Fuctional Constipation

1.
College of Acupuncture Moxibustion and Tuina, Shaanxi University of Chinese Medicine, Xianyang, 712046, China
2.
Clinical Medical College, Xi'an Medical University, Xi'an, 710021, China
3.
Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, 712000, China
4.
Department of TCM Acupunture and Physiotherapy, Xi'an Aerospace General Hospital, Xi'an, 710100, China

摘要

摘要: 目的探讨电针不同穴组治疗功能性便秘（FC）大鼠的作用机理。方法 FC动物模型采用0 ℃ 0.9%氯化钠溶液对SD大鼠灌胃复制，随机分为模型组、电针1组（EAⅠ组）、电针2组（EAⅡ组）和电针3组（EAⅢ组），正常组采用SD大鼠。EAⅠ组取天枢、大肠俞（双侧）、EAⅡ组取曲池、上巨虚（双侧）、EAⅢ组取天枢、大肠俞、曲池、上巨虚（单侧）进行电针治疗。观测粪便性状和肠道炭末推进率评定大鼠肠动力，透射电镜检测大鼠结肠黏膜超微结构，Western blot检测大鼠结肠AQP3、AQP8蛋白表达，qPCR检测大鼠结肠AQP3、AQP8 mRNA表达。结果 EAⅡ组大鼠24 h粪便粒数、24 h粪便含水量和肠道炭末推进率均显著增加，首次黑便时间显著减少（P<0.05），结肠黏膜超微结构经EAⅡ干预后显著改善，模型组大鼠结肠黏膜AQP3、AQP8蛋白和AQP3、AQP8 mRNA表达显著高于正常组（P<0.05），经EAⅡ干预后表达显著降低，具有统计学意义（P<0.05）。结论电针刺激曲池、上巨虚可能通过降低FC大鼠结肠黏膜AQP3、AQP8蛋白和AQP3、AQP8 mRNA表达，减少结肠水分重吸收，增加结肠粪便含水量，调节水分转运，改善结肠黏膜超微结构，增强肠动力，起到改善便秘症状的作用。
- 功能性便秘 /
- 超微结构 /
- AQP3 /
- AQP8 /
- 电针
Abstract: OBJECTIVE To explore the mechanism of electroacupuncture at different points combination in the treatment of rats with functional constipation (FC). METHODS SD rats replicated by intragastric administration with 0 ℃ 0.9% sodium chloride solution were taken as FC animal model and randomly divided into model group， electroacupuncture group Ⅰ， electroacupuncture group Ⅱ and electroacupuncture group Ⅲ， SD rats were taken as control group. EAⅠhad electroacupuncture at bilateral Tianshu(ST 25)， Dachangshu(BL 25)， EAⅡ had electroacupuncture at bilateral Quchi(LI 11)， Shangjuxu(ST 37)， EAⅢ had electroacupuncture at unilateral Tianshu(ST 25)， Dachangshu(BL 25)， Quchi(LI 11)， Shangjuxu(ST 37). Observation on fecal characteristics and intestinal carbon propelling rate were used to evaluate intestinal motility of rats， transmission electron microscopy was used to observe colonic mucosa ultrastructure of rats， Western blot was used to determine the protein expression of AQP3， AQP8 in colon of rats， qPCR was used to determine the mRNA expression of AQP3， AQP8 in colon of rats. RESULTS The number of 24 h fecal grains， 24 h fecal water content and intestinal carbon propelling rate in EA Ⅱ group increased significantly(P<0.05)， protein and mRNA expression of AQP3， AQP8 in the model group was significantly higher than that of normal group in colon of rats， while significantly reduced after treatment with EA Ⅱ(P<0.05)， the ultrastructure of colonic mucosa were significantly improved after treatment with EA Ⅱ. CONCLUSION The mechanism of electroacupuncturestimulating Quchi(LI 11)， Shangjuxu(ST 37) on enhancing intestinal motility and improving the symptoms of constipation may be due to the reducing the expression of AQP3， AQP8 protein and AQP3， AQP8 mRNA in colonic mucosa of FC rats， thus reducing water reabsorption of colon， increasing fecal water content in colon， regulating water transport and improving the ultrastructure of colonic mucosa.
- functional constipation /
- ultrastructure /
- AQP3 /
- AQP8 /
- electroacupuncture

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参考文献(7)

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