醋制对商陆促利尿作用的影响及其机制研究
Study on Diuretic Effects and Its Mechanism of Phytolaccae Radix Before and After Vinegar Processing
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摘要: 目的 探索毒性中药商陆醋制前后对机体利尿作用及相关指标的影响。方法 灌胃给予盐水负荷模型大鼠商陆醋制前后的水煎液,测定给药5 h内尿量变化、血浆抗利尿激素(ADH)、醛固酮(ALD)、心房钠尿肽(ANP)含量及肾脏水通道蛋白AQP2、AQP3、AQP4表达水平。结果 商陆醋制前后均能导致大鼠尿量显著增加(P<0.01),醋制品较生品组大鼠尿量有显著增强(P<0.05);商陆生品仅高剂量可显著降低血浆ADH水平(P<0.01),同时有降低ALD、增加ANP水平的趋势,但无显著性差异;商陆醋品则可显著降低血浆ADH、ALD水平,升高ANP含量(P<0.05~0.01);商陆生品可显著抑制肾脏中AQP2、AQP3和AQP4蛋白表达(P<0.05~0.01),醋制后可显著抑制AQP2、AQP3蛋白表达水平(P<0.01),对AQP4蛋白表达与模型组相比无显著性影响,且醋制品较生品抑制AQP3蛋白表达更显著(P<0.05)。结论 商陆经醋制后仍具有较强的利尿作用,且分子水平的效应比生品更强。商陆利尿作用的机制除与其改变机体体液代谢相关的激素水平外,还可能与其抑制肾脏远曲小管和集合管AQPs蛋白表达,进而抑制水的重吸收有关。Abstract: OBJECTIVE To investigate the effects of phytolaccae radix before and after vinegar processing on diuretic effects and related factors. METHODS Saline-loaded model rats were given different kinds of phytolaccae radix decoction by gavage to detect the changes of urine volume within 5 h, plasma concentrations of ADH, ALD, ANP and protein expression levels of AQP2, AQP3 and AQP4. RESULTS Phytolaccae radix before and after vinegar processing significantly increased the urine volumes of rats (P<0.01) and vinegar product significantly increased urine volume compared to raw product (P<0.05). Only high dose of raw product significantly reduced plasma concentration of ADH (P<0.01) and caused a tendency to reduce ALD and increase ANP content, without significant difference. Vinegar product significantly reduced plasma concentrations of ADH, ALD and increased ANP content (P<0.05, P<0.01). Raw product significantly inhibited the protein expressions of AQP2, AQP3 and AQP4 (P<0.05, P<0.01) while vinegar product significantly inhibited the protein expressions of AQP2 and AQP3 (P<0.01), whereas showed no significant influence on AQP4 protein expression, compared to control group. Moreover, vinegar product inhibited the protein expression of AQP3 more significantly than raw product (P<0.05). CONCLUSION Phytolaccae radix after vinegar processing still retains diuretic effect and its efficacy is better than that of raw product at the molecular levels. The diuretic mechanism of phytolaccae radix maybe associated with changing hormone levels of fluid metabolism, and the inhibition of AQPs protein expressions in renal distal convoluted tubules and collecting ducts, which can inhibit water reabsorption.
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Key words:
- Phytolaccae Radix /
- vinegar processing /
- diuretic effect /
- ADH /
- ALD /
- ANP /
- aquaporins
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