山羊角中巯基的测定及山羊角对体内巯基的干预研究
Determination of Thiol in Caprae Hircus Cornu and Its Effect on Thiol Level in Rat Plasma
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摘要: 目的 测定山羊角提取液及大鼠血浆中游离巯基（-SH）与总-SH的含量，探讨山羊角对大鼠体内-SH干预情况。方法 采用Ellman法测定山羊角提取液中游离-SH与总-SH的含量；大鼠连续灌胃给予山羊角提取物后，测定大鼠血浆中游离-SH和总-SH的水平；大鼠灌胃7 d给予山羊角提取物后，连续测定给药后大鼠血浆中游离-SH含量随时间变化情况。结果 山羊角提取液中的游离-SH含量介于3.96~5.08 mmol/L，总-SH的含量介于4.52~5.94 mmol/L；连续给予大鼠山羊角提取液后，第4天始，大鼠血浆中游离-SH与总-SH水平相较于正常大鼠显著提升（P<0.01），而阿司匹林对大鼠血浆中的游离-SH与总-SH水平无影响；口服给予大剂量山羊角后30 min，大鼠血浆中游离-SH含量即达最大值(10.16±1.18)mmol/L，8 h后大鼠血浆中游离-SH含量基本降至正常大鼠水平。结论 山羊角提取液中含有丰富的-SH，且口服山羊角后血浆游离-SH与总-SH水平均显著提升，提示山羊角中含SH基团的一类成分可能为山羊角重要功效物质基础，为角类动物药功效物质基础及效应特点研究提供借鉴与思路。Abstract: OBJECTIVE To determine the level of native thiols (-SH) and total -SH in goat horn extract and rat plasma. METHODS The thiols levels in goat horn extract and rat plasma were measured using the classical Ellman reagent method. Both the levels of native -SH and total -SH in rat plasma were measured after oral administration of goat horn extract. After oral administration of goat horn extract， the native -SH level in rat plasma was measured， and the curve of -SH level against time after administration was obtained. RESULTS The level of native -SH in goat horn extract ranged from 3.96 to 5.08 mmol/L， and the level of total -SH in goat horn extract ranged from 4.52-5.94 mmol/L. After continuously oral administration of goat horn extract， both the levels of native -SH and total -SH in rat plasma significantly increased (P<0.01) compared with normal rat from d4 to d7. Oral administration of Aspirin did not significantly increase the -SH level in rat plasma. In addition， 30 min after oral administration of goat horn extract， the level of native -SH in rat plasma significantly increased to (10.16±1.18)mmol/L， and 8 h later， the level of native -SH in plasma reverted to normal level. CONCLUSION There are abundant -SH contained components in goat horn extract， and goat horn extract could obviously increase the -SH level in rat plasms. The results in the present study suggested that -SH group contained components might be the important material basis of goat horn. It provides a reference for investigations on animal horn derived TCMs.
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Key words:
- Caprae hircus cornu /
- thiol /
- plasma /
- determination
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