糖可明对糖尿病视网膜病变中血-视网膜屏障的影响

孙利; 丁永芳

糖可明对糖尿病视网膜病变中血-视网膜屏障的影响

孙利^1,2,
丁永芳^1,2,

1.
南京中医药大学附属中西医结合医院，江苏南京 210028
2.
江苏省中医药研究院药理与毒理研究室，江苏南京 210028

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出版历程
- 刊出日期: 2018-07-10

Effect of Tangkeming on Blood-Retinal Barrier in Diabetic Rats with Retinopathy

SUNLi^1,2,
DINGYong-fang^{1,2
,}

1.
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, China
2.
Pharmacology of Toxicology Laboratory, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China

摘要

摘要: 目的探讨糖可明对糖尿病视网膜病变的影响。方法用链脲佐菌素（STZ）复制大鼠糖尿病视网膜病变模型，观察糖可明对模型动物血液生化、血-视网膜屏障(Blood-retinal barrier，BRB)渗漏和视网膜VEGF、occludin表达的影响。结果糖可明连续给药8周后，可明显降低GLU、TG和TC的含量（P<0.05～0.01）；降低血-视网膜屏障的通透性；PCR结果显示，给药组大鼠视网膜occludin mRNA表达量显著增加（P<0.05～0.01）；免疫组化实验发现糖可明可显著降低视网膜全层的VEGF表达（P<0.01）。结论糖可明通过下调糖尿病大鼠视网膜VEGF的表达和增加occludin的表达，从而发挥其保护BRB、降低BRB通透性的作用，延缓糖尿病视网膜病变的进程。
- 糖可明 /
- 糖尿病视网膜病变 /
- 血-视网膜屏障
Abstract: OBJECTIVE To study the effect of Tangkeming (TKM) on blood-retinal barrier (BRB) in diabetic rats. METHODS Diabetic retinopathy rats were induced by STZ. The blood biochemical indexes were detected. Retinal vascular permeability was measured by Evans blue. The expression of occludin and VEGF in retina of diabetic rats were determined by qPCR and immunohistochemistry， respectively. RESULTS Administration of TKM to diabetic retinopathy rats significantly ameliorated the levels of GLU， TG and TC in serum. The Evans blue contents in dry retina tissue remarkedly reduced upon TKM treatment. The level of occludin mRNA was up-regulated and the expression of VEGF in retina was down-regulated by TKM in diabetic rats. CONCLUSION TKM protects the BRB by inhibiting expression of VEGF associated with occludin.
- Tangkeming /
- diabetic retinopathy /
- blood-retinal barrier

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参考文献(10)

[1]	曹建峰，庞东渤，叶文婕. HIF1α及VEGF在增生性糖尿病视网膜病变视网膜前膜中的表达[J].国际眼科杂志，2013，13(5):857860.〖ZK)〗
[2]	魏燕华，成差群，徐建平. 枸杞多糖对2型糖尿病大鼠视网膜炎症因子表达的影响[J]. 中国药业， 2014， 23(9):1214.〖ZK)〗
[3]	李静文，李国文，陆骏，等. 石斛多糖对糖尿病大鼠血视网膜屏障的保护作用及其机制[J]. 中华实验眼科杂志， 2017， 35(7):611616.〖ZK)〗
[4]	顾永昊，柯根杰，王林，等. 基质金属蛋白酶抑制剂GM6001对糖尿病大鼠血视网膜屏障的影响[J]. 中华实验眼科杂志，2014， 32(11)：10101013.〖ZK)〗
[5]	HAUTALA N， AIKKILA R， KORPELAINEN J， et al. Marked reductions in visual impairment due to diabetic retinopathy achieved by efficient screening and timely treatment[J]. Acta Ophthalmol， 2014， 92(6):582587.〖ZK)〗
[6]	MA Y， ZHANG Y， ZHAO T， et al. Vascular endothelial growth factor in plasma and vitreous fluid of patients with proliferative diabetic retinopathy patients after intravitreal injection of bevacizumab[J]. Am J Ophthalmol， 2012， 153(2):307313.〖ZK)〗
[7]	MARK KS， DAVIS TP. Cerebral microvascular changes in permeability and tight junctions induced by hypoxiareoxygenation[J]. Am J Physiol Heart Circ Physiol， 2002， 282(4): 14851494.〖ZK)〗
[8]	ANTONETTI DA， BARBER AJ， KHIN S， et al. Vascular permeability in experimental diabetes is associated with reduced endothelial occludin content: vascular endothelial growth factor decreases occludin in retinal endothelial cells. Penn State Retina Research Group[J]. Diabetes， 1998， 47(12):19531959.〖ZK)〗
[9]	PENG H， WANG C， YE ZC， et al. How increased VEGF induces glomerular hyperpermeability: a potential signaling pathway of Rac1 activation[J]. Acta Diabetol， 2010， 47 (S1):5763.〖ZK)〗
[10]	SPOERRI PE， AFZAL A， LI CALZI S， et al. Effects of VEGFR1， VEGFR2， and IGFIR hammerhead ribozymes on glucosemediated tight junction expression in cultured human retinal endothelial cells[J]. Mol Vis， 2006， 12:3242.〖ZK)〗