Abstract: OBJECTIVE To investigate the damage effects of puromycin aminonucleoside (PAN) on human podocyte and the repair effect of astragaloside Ⅳ. At the same time，to explore whether PKC/MAPK pathway is involved in it to seek possible mechanisms. METHODS To filter appropriate concentration of model establishment of PAN and the maximum non-toxic concentration of astragaloside Ⅳ by cytotoxicity test CCK-8.In the experiment，PAN was used to cause the damage of human podocytes and then different concentrations of astragaloside Ⅳ was treated with cells.Finally，the protein expression levels of nephrin， podocin， PKC， JNK and p38 were detected by Western blotting. RESULTS The protein expression levels of nephrin and podocin were declined as where as PKC， JNK and p38 were ascended obviously after stimulating by PAN and tended to nomal gradually after treatment with astragaloside Ⅳ，furthermore，these changes were in a dose-dependent manner.The difference was statistically significant (P<0.05).However，the changes between solvent control group and model group were unconspicuous， which showed no significant difference. CONCLUSION PAN can cause the damage of human podocyte slit diaphragm and astragaloside Ⅳ can repair the injury which is positively correlated with astragaloside Ⅳ. Meanwhile， PKC/MAPK pathway is involved in the repair process.This may be the possible mechanism of Astragaloside Ⅳ on the repair of human podocyte.