杂色蛤核苷部位的保肝作用研究

倪骏; 张倩; 刘睿; 王欣之; 程建明; 卞慧敏; 王青; 吴皓

杂色蛤核苷部位的保肝作用研究

倪骏^1,2,
张倩^1,2,
刘睿^1,2,
王欣之^1,2,
程建明^1,2,
卞慧敏^1,2,
王青^1,2,
吴皓^1,2,

1.
南京中医药大学江苏省海洋药用生物资源研究与开发重点实验室，江苏南京 210023
2.
江苏省中药资源产业化过程协同创新中心，江苏南京 210023

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出版历程
- 刊出日期: 2018-05-10

Study on Liver Protection of Nucleosides from Ruditape Philippinarum

NIJun^1,2,
ZHANGQian^1,2,
LIURui^1,2,
WANGXin-zhi^1,2,
CHENGJian-ming^1,2,
BIANHui-min^1,2,
WANGQing^1,2,
WUHao^{1,2
,}

1.
Jiangsu Key Laboratory of Research and Development in Marine Bio-Resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing, 210023, China

摘要

摘要: 研究杂色蛤核苷类部位在体内、体外的保肝作用。方法采用CCK-8法测定核苷对细胞存活率的影响，四氯化碳(CCl4)诱导肝细胞损伤和过氧化氢（H2O2）诱导肝细胞损伤的体外实验以及CCl4诱导急性化学性肝损伤模型和D-半乳糖致衰老抗氧化模型的体内实验来测定谷丙转氨酶（ALT）、谷草转氨酶（AST）、超氧化物歧化酶（SOD）、丙二醛（MDA）、还原性谷胱甘肽（GSH-Px）来评价杂色蛤核苷部位的保肝作用。结果杂色蛤核苷部位可显著提高肝细胞的存活率，显著降低肝损伤的ALT、AST水平，显著升高SOD的活性，降低MDA的含量（P<0.05~0.01）。结论杂色蛤核苷部位可保护CCl4诱导的小鼠化学性肝损伤，对抗D-半乳糖致小鼠衰老，在体外对CCl4、H2O2诱导的肝细胞均有显著的保护作用，为开发和利用杂色蛤资源，研制保肝产品提供了实验依据。
- 杂色蛤 /
- 保肝 /
- 核苷 /
- 四氯化碳
Abstract: OBJECTIVE To investigate the liver protection of nucleobases from Ruditapes philippinarum in vivo and in vitro. METHODS CCK-8 assay was used to detect cell viability. The model of carbon tetrachloride(CCl4) and hydrogen peroxide(H2O2)-induced chemical liver injury in primary cultured hepatocytes were prepared in vitro. The model of carbon tetrachloride and D-galactose-induced liver injury in mice were built in vivo. The levels of ALT， AST， MDA， SOD and GSH-Px activities were measured to evaluate liver protection of nucleosides from Ruditapes philippinarum. RESULTS The nucleoside of Ruditapes philippinarum significantly increased the survival rate of liver cells. The drug groups significantly decreased the activities of AST and ALT， significantly increased the activity of SOD and significantly reduced the level of MDA. CONCLUSION Nucleosides from Ruditapes philippinarum show hepatoprotective effects on CCl4-induced chemical liver injury， D- galactose induced aging， and liver cells injured by CCl4 and H2O2. This study provided experimental data for the development and utilization of Ruditapes philippinarum resources and new hepatoprotective medicines.
- Ruditapes philippinarum /
- liver protection /
- nucleosides /
- carbon tetrachloride