龟鹿二仙胶对地塞米松诱导骨髓间充质干细胞损伤的保护作用

徐卫峰; 费逸明; 王建伟; 尹恒; 王善付; 葛卫红

龟鹿二仙胶对地塞米松诱导骨髓间充质干细胞损伤的保护作用

1.
南京医科大学药学院，江苏南京 211166
2.
南京中医药大学无锡附属医院，江苏无锡 214071
3.
南京大学医学院附属鼓楼医院，江苏南京 210008

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出版历程
- 收稿日期: 2017-03-11
- 修回日期: 2017-05-28
- 刊出日期: 2017-07-10

Effects and Mechanisms for Guilu Erxian Glue on Dexamethasone Induced Bone Marrow Mesenchymal Stem Cells Injury

1.
School of Pharmacy, Nanjing Medical University, Nanjing, 211166, China
2.
Wuxi Affiliated Hospital of Nanjing University of Chinese Medicine, Wuxi, 214071, China
3.
Nanjing Drum Tower Affiliated Hospital of Nanjing University Medical School, Nanjing, 210008, China

摘要

摘要: 目的研究龟鹿二仙胶对地塞米松诱导骨髓间充质干细胞损伤的保护作用。方法体外培养SD大鼠骨髓间充质干细胞，传至第二代后分5组培养：正常组、地塞米松（10-8mol/L）组、骨化三醇含药血清组、龟鹿二仙胶含药血清低剂量组、龟鹿二仙胶含药血清高剂量组。MTT法检测骨髓间充质干细胞活力，检测细胞上清中的超氧化物歧化酶（SOD）、丙二醛（MDA）、炎症因子白介素-6（IL-6）、小鼠白介素1β（IL-1β）、肿瘤坏死因子-α（TNF-α）。采用Western blot检测Nrf-2/HO-1/NF-κB信号通路蛋白表达。结果龟鹿二仙胶能显著提高细胞活力，增加SOD水平、降低MDA水平、降低炎症因子水平，增加蛋白Nrf-2、HO-1蛋白表达水平，降低NF-κB信号蛋白表达。结论龟鹿二仙胶对地塞米松诱导骨髓间充质干细胞损伤有保护作用，且作用与调控Nrf-2/HO-1/NF-κB信号相关。
- 龟鹿二仙胶 /
- 地塞米松 /
- 骨髓间充质干细胞 /
- 氧化应激 /
- 炎症
Abstract: OBJECTIVE The protective effect of Guilu Erxian Glue on dexamethasone induced bone marrow mesenchymal stem cells injury. METHODS Bone marrow mesenchymal stem cells were obtained from SD rats and divided into four groups: normal group， dexamethasone (10-8) group， low dose and high dose of Guilu Erxian Glue groups. The activity of bone marrow mesenchymal stem cells was measured by MTT assay， superoxide dismutase (SOD)， malondialdehyde (MDA)， interleukin-6 (IL-6)， IL-1β， tumor necrosis factor a (TNF-α) were measured. The expression of Nrf-2/HO-1/NF-κB signal proteins was detected by Western blot. RESULTS Guilu Erxian Glue groups can significantly improve cell vitality， increase SOD level， reduce the level of MDA， reduce the level of inflammation factor， increase the expression of Protein， Nrf-2 and HO-1 proteins， and reduce the expression of NF-κB signal proteins. CONCLUSION Guilu Erxian Glue can inhibit the injury of bone marrow mesenchymal stem cells by dexamethasone， and the role is related to the regulation of Nrf-2/HO-1/NF-κB signal.
- Guilu Erxian Glue /
- dexamethasone /
- bone marrow mesenchymal stem cells /
- oxidative stress /
- inflammation

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参考文献(9)

[1]	JANDEROVA L， MCNEIL M， MURRELL AN， et al. Human mesenchymal stem cells as an in vitro model for human adipogenesis[J]. Obes Res， 2003， 11（1）: 65-74.
[2]	HUNG SC， CHANG CF， MA HL， et al. Gene expression profiles of early adipogenesis in human mesenehymal stem cells[J]. Gene， 2004， 340（1）: 141-150.
[3]	ZHANG S， LI D， YANG JY， et al. Plumbagin protects against glucocorticoid-induced osteoporosis through Nrf-2 pathway[J]. Cell Stress Chaperones， 2015， 20（4）: 621-629.
[4]	LIU YQ， HONG ZL， ZHAN LB， et al. Wedelolactone enhances osteoblastogenesis by regulating Wnt/β-catenin signaling pathway but suppresses osteoclastogenesis by NF-κB/c-fos/NFATc1 pathway[J]. Sci Rep， 2016， 25（6）: 32260.
[5]	BAEK KH， OH KW， LEE WY， et al. Association of oxidative stress with postmenopausal osteoporosis and the effects of hydrogen peroxide on osteoclast formation in human bone marrow cell cultures[J]. Calcif Tis Int， 2010， 87（3）: 226-235.
[6]	CERVELLATI C， BERGAMINI CM. Oxidative damage and the pathogenesis of menopause related disturbances and diseases[J]. Clin Chem Lab Med， 2016， 54（5）: 739-753.
[7]	LIU YJ， ZHOU CY， QIU CH， et al. Chlorogenic acid induced apoptosis and inhibition of proliferation in human acute promyelocytic leukemia HL60 cells[J]. Mol Med Rep， 2013， 8（4）:1106.
[8]	LEOTOING L， WAUQUIER F， GUICHEUX J， et al. The polyphenol fisetin protects bone by repressing NF-κB and MKP-1-dependent signaling pathways in osteoclasts[J]. Plos One， 2013， 8（7）: e68388.
[9]	LIOU SF， HSU JH， LIN IL， et al. KMUP-1 suppresses RANKL-induced osteoclastogenesis and prevents ovariectomy-induced bone loss: roles of MAPKs， Akt， NF-κB and calcium/calcineurin/NFATc1 pathways[J]. Plos One， 2013， 8（7）:e69468.