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双氢青蒿素对胆管结扎致大鼠肝纤维化的治疗作用研究

许霞 付金柏 姚真 陈一凡 钱磊 江朔仪 周静怡 郑仕中

许霞, 付金柏, 姚真, 陈一凡, 钱磊, 江朔仪, 周静怡, 郑仕中. 双氢青蒿素对胆管结扎致大鼠肝纤维化的治疗作用研究[J]. 南京中医药大学学报, 2017, 33(4): 376-381.
引用本文: 许霞, 付金柏, 姚真, 陈一凡, 钱磊, 江朔仪, 周静怡, 郑仕中. 双氢青蒿素对胆管结扎致大鼠肝纤维化的治疗作用研究[J]. 南京中医药大学学报, 2017, 33(4): 376-381.
XUXia, FUJin-bai, YAOZhen, CHENYi-fan, QIANLei, JIANGShuo-yi, ZHOUJing-yi, ZHENGShi-zhong. Therapeutical Effect of Dihydroartemisinin on Bile Duct Ligation-Induced Liver Fibrosis in Rats and Its Mechanism[J]. Journal of Nanjing University of traditional Chinese Medicine, 2017, 33(4): 376-381.
Citation: XUXia, FUJin-bai, YAOZhen, CHENYi-fan, QIANLei, JIANGShuo-yi, ZHOUJing-yi, ZHENGShi-zhong. Therapeutical Effect of Dihydroartemisinin on Bile Duct Ligation-Induced Liver Fibrosis in Rats and Its Mechanism[J]. Journal of Nanjing University of traditional Chinese Medicine, 2017, 33(4): 376-381.

双氢青蒿素对胆管结扎致大鼠肝纤维化的治疗作用研究

Therapeutical Effect of Dihydroartemisinin on Bile Duct Ligation-Induced Liver Fibrosis in Rats and Its Mechanism

  • 摘要: 目的 探究双氢青蒿素(Dihydroartemisinin,DHA)对胆管结扎(Bile duct ligation, BDL)所致的大鼠肝纤维化的初步治疗作用。方法 采用BDL诱导大鼠肝纤维化模型,DHA(14mg/kg)及阳性药物秋水仙碱(0.1mg/kg)干预处理,末次给药24h后,取血清检测ALT、AST、Bilirubin;HE染色、马松染色和天狼猩红染色检测肝组织病理变化;Western blot检测肝组织中肝星状细胞(HSC)活化指标α-SMA与α1(Ⅰ) collagen的表达;免疫荧光检测肝组织TGFβ-RⅡ的表达。体外培养活化的HSC-T6,并用DHA干预处理,Western blot检测α-SMA、α1(Ⅰ) collagen以及TGFβ-RⅡ、p-Smad2、Smad2的表达。结果 血清学指标结果显示,与空白对照组比较,模型组SD大鼠ALT、AST、Bilirubin显著升高,DHA能降低血清中ALT、AST、Bilirubin水平;HE、马松染色、天狼猩红染色结果显示,与模型组比较,DHA能够明显改善肝脏病理组织结构以及胶原纤维的沉积;Western blot结果显示,DHA还可抑制HSC活化的标志物α-SMA、α1(Ⅰ) collagen以及TGFβ-RⅡ、p-Smad2的表达。结论 DHA对BDL诱导的肝纤维化具有显著的保护作用,这一效应主要与其抑制HSC活化以及调控TGF-βⅡ/Smad2信号通路相关。

     

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出版历程
  • 收稿日期:  2017-03-09
  • 修回日期:  2017-06-28
  • 刊出日期:  2017-07-10

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