一测多评法在消癥胶囊质量控制中的应用
Application of Quantitative Analysis Multi-Components by Single Marker in Xiaozheng capsule Quality Control
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摘要: 目的 建立一测多评法测定消癥胶囊中5种大黄蒽醌类成分的含量。方法 选择大黄素为内参物,建立芦荟大黄素、大黄酸、大黄酚、大黄素甲醚与大黄素的相对校正因子(fk/s),并计算含量,实现一测多评;同时与外标测定法的结果进行对比,以验证一测多评法可行性。结果 在一定的线性范围内,3批消癥胶囊中5种大黄蒽醌类成分所得fk/s计算值与实测值间无显著差异。结论 一测多评法快速、准确,可用于消癥胶囊中大黄蒽醌类成分的质量评价。Abstract: OBJECTIVE To establish a method of quantitative analysis on multi-components by single marker (QAMS) to determine 5 kinds of anthraquinones in Xiaozheng capsule. METHODS Using emodin as internal referring substance,to establish the relative correction factor (fk/s) of Aloe Emodin, rhein, chrysophanol, physcion to emodin and determine these contents. At the same time, an external standard method was established to determine the amounts of 5 kinds of anthraquinones, then compared with the QAMS method. RESULTS The two methods did not show significant difference in assay results according to the data in a certain linear range. CONCLUSION The QAMS method is feasible and credible, and can be used to determine and control the 5 kinds of anthraquinones in Xiaozheng capsule.
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