番荔枝子油提取工艺研究

殷畅; 瞿敏明; 孙荣蔚; 钱阳艳; 孙红; 胡云峰; 李祥; 陈建伟; 陈勇

番荔枝子油提取工艺研究

1.
南京中医药大学药学院，江苏南京 210023
2.
上海市纳米科技与产业发展促进中心，上海 200237

计量
- 文章访问数: 867
- HTML全文浏览量: 6
- PDF下载量: 622
- 被引次数: 0
出版历程
- 刊出日期: 2016-07-10

Optimization of Extraction Technique of Seed Oil from Annona squamosa

1.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
Shanghai Nanotechnology Promotion Center, Shanghai, 200237, China

摘要

摘要: 目的优化番荔枝子油的提取工艺。方法采用渗漉法、索式提取法、超声波萃取法和超临界CO₂萃取法提取番荔枝子油，以番荔枝种子的出油率为指标，确定最佳提取方法；对最佳提取方法进行工艺的优化，并采用GC-MS法分析其化学成分。结果索氏提取法提取的番荔枝子油得率最高；索式提取法提取番荔枝子油的最佳工艺为：采用石油醚为提取溶剂，提取温度为80℃，回流时间为90min，番荔枝子油的出油率为（22.28±0.21）%。GC-MS分析结果表明，最佳工艺所得的番荔枝子油的主要成分及相对含量为：棕榈酸（9.92%）、亚油酸（20.49%）、油酸（56.50%）及硬脂酸（9.14%）。结论该实验优选出的索氏提取工艺稳定，番荔枝子油中不饱和脂肪酸的含量较高，具有一定的经济应用价值。
- 番荔枝子 /
- 索式提取 /
- GC-MS
Abstract: OBJECTIVE To determine the optimal parameters in extraction of seed oil from Annona squamosa. METHODS The extraction efficiency of four extraction methods， including percolation extraction， soxhlet extraction， ultrasonic extraction and SFE-CO₂ extraction were compared. The optimal parameters of the best extraction method were determined， and the chemical composition of the oils was analyzed by GC-MS. RESULTS The yields of the oils extracted by the soxhlet extraction method were highest (22.28±0.21%)， and the optimal parameters were obtained as follows: Petroleum ether were used as extraction solvent， the extraction temperature was 80℃， and the extraction time was 90 min. GC-MS analysis results showed that the main chemical composition was palmitic acid (9.92%)， linoleic acid (20.49%)， oleic acid (56.50%) and stearic acid (9.14%). CONCLUSION The optimum extraction method was stable. Annona squamosa seeds are rich in unsaturated fatty acids and have a certain economic value.
- Annona squamosa seeds /
- Soxhlet extraction /
- GC-MS

HTML全文

参考文献(9)

[1]	广东省食品药品管理局. 广东省中药标准：第一册[S]. 广州: 广东科技出版社， 2004: 194.
[2]	Guangdong Food and Drug Administration. Guangdong Chinese Materia Medica standards： Volume Ⅰ[S]. Guangdong: Guangdong science and technology press， 2004: 194.
[3]	LIMAS LARS， PIMENTA LPS， BOAVENTURA AD. Acetogenins from Annona cornifolia and their antioxidant capacity[J]. Food Chem， 2010， 122(4): 1129-1138.
[4]	LI CM， TAN NH， ZHENG HL， et al. Cyclopeptide from the seeds Annona glabr[J]. Phytochemistry， 1999， 50(6): 1047-1052.
[5]	田丽鹃，杨念云，孟正木，等. 圆滑番荔枝的化学成分研究[J]. 中国药学杂志， 2003， 38(4): 258-260.
[6]	TIAN LJ， YANG NY， MENG ZM， et al. Studies on the chemical constituents of Annona glabra[J]. Chin Pharm J， 2003， 38(4): 258-260.
[7]	邱燕，陈勇，陈建伟，等. 番荔枝子脂肪油化学成分及其抗肿瘤活性[J]. 中国实验方剂学杂志， 2014， 20（21）: 109-111.
[8]	QIU Y， CHEN Y， CHEN JW， et al. Chemical constituents and antitumor activity of fatty oil from Annona squamosa seed[J]. Chin J Exp Tradit Med Form， 2014， 20(21): 109-111.
[9]	YANG HJ， LI X， TANG YP， et al. Supercritical fluid CO2 extraction and simultaneous determination of eight annonaceous acetogenins in Annona genus plant seeds by HPLC-DAD method[J]. J Pharm Biomed Anal， 2009， 49: 140-144.