Abstract: OBJECTIVE To establish an ovarian cancer xenograft model by fluorescence and study of the cancer staging. METHODS The human ovarian cancer HO-8910 cells were cultured for 10 generations in the cell culture dish， and the cells that could stably express fluorescence were selected and injected into nude mice. When the tumor grew to a mass volume of 1mm3， it was implanted into the ovarian mucous membrane of nude mice through micro-surgery. The tumor growth and metastasis were observed by in vivo imaging technique， and the tumor tissue and surrounding tissues were observed through pathology detection methods. RESULTS In vivo imaging technology successfully traced the development and metastasis of tumor. Pathological examination showed tumor cell metastasis trend. Both of them combining with TCM clinical ovarian cancer staging determined the ovarian cancer staging. CONCLUSION The experiment successfully establishes ovarian cancer orthotopic transplantation model and represents the evolution of clinical oncology.The stages will provide some experimental basis for the study of tumor and the evaluation criterion of antitumor drug effect.