菖蒲郁金汤对高皮质激素血症小鼠海马NF-L及SYP蛋白表达的影响
Effect of Acorus and Curcuma Decoction on Cortical Hormone in Mice Hippocampus NF-L and SYP Protein
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摘要: 目的 探讨菖蒲郁金汤拮抗皮质酮诱导海马神经元损伤的作用。方法 将C57BL/6N小鼠随机分为空白组、模型组、氟西汀组、菖蒲郁金汤高剂量组、菖蒲郁金汤中剂量组及菖蒲郁金汤低剂量组。除空白组外,其余各组通过皮质酮诱导高皮质激素血症,予受试药物连续14d,观察小鼠体质量、脾脏质量及胸腺质量变化,采用强迫游泳实验和强迫悬尾实验评价菖蒲郁金汤对小鼠不动时间的影响,Western blot法检测神经微丝轻链蛋白(NF-L)及突触囊泡蛋白(SYP)蛋白表达。结果 菖蒲郁金汤可缓减模型动物体质量、脾脏质量及胸腺质量的降低(P<0.05);减少强迫游泳实验、强迫悬尾实验小鼠不动时间(P<0.01);上调NF-L及SYP蛋白的表达(P<0.01)。结论 菖蒲郁金汤通过上调NF-L及SYP的表达,拮抗高皮质激素血症所致的海马神经元损伤,发挥抗抑郁样行为效应。Abstract: OBJECTIVE To investigate the reverse effect of Acorus and Curcuma Decoction on despair like behavior in mice induced by hypercortisolism. METHODS 60 C57BL/6N mice were randomly divided into 6 groups: normal control, model control, Fluoxetine hydrochloride group, Acorus and Curcuma Decoction groups of high dose, middle dose and low dose. The hypercortisolism mice models were developed by subcutaneous corticosterone injection. Four weeks later, the changes of mice body, spleen and thymus mice weight were detected. After the forced swimming test and the tail suspension test finished, the mice were sacrificed by decapitation and the hippocampal tissues were rapidly collected for Western blot analysis of neurofilament light chain protein(NF-L) and synaptic vesicle protein(SYP). RESULTS Compared with the normal control group, the body weight was lower than model control group(P<0.05), the immobility time was significantly prolonged in the forced swimming test and the tail suspension test (P<0.01), spleen and thymus weight significantly decreased (P<0.05), the expression of NF-L and SYP, were down-regulated in model control group(P<0.01). However, Acorus and Curcuma Decoction group, significantly shortened immobility time in behavioral science(P<0.01), and up-regulated the expression of NF-L and SYP (P<0.01). CONCLUSION Acorus and Curcuma Decoction can protect neurons and antagonized the despair like behavior induced by hypercortisolism.
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Key words:
- Acorus and Curcuma Decoction /
- high blood cortical hormone /
- behavior /
- synaptophysin
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[1] Chen L, Dai J, Wang Z, et al. The antidepressant effects of ginseng total saponins in male C57BL/6N mice by enhancing hippocampal inhibitory phosphorylation of GSK-3β[J]. Phytother Res, 2014, 28(7): 1102-1106. [2] 戴建国,王中立,陈琳,等.远志-茯神配伍预防高皮质激素血症致海马神经可塑性损伤作用研究[J].南京中医药大学学报,2015, 31(3):242-245. [3] Dai JG, Wang ZL, Chen L, et al. Preventive effect of compatibility of radix polygalae and poria cum radix Pini on hippocampalm neuronal plasticity damage induced by hypercortisolism[J]. J Nanjing Univ Tradit Chin Med, 2015, 31(3): 242-245. [4] 张惠珍,孙林.加味菖蒲郁金汤对抑郁大鼠模型行为学的影响[J].甘肃中医,2010, 23(3):29-30. [5] Zhang HZ, Sun L. Effect of Changpu Yujin Decoction on the behaviors in rats with depression model[J]. Gansu J Tradit Chin Med, 2010, 23(3): 29-30. [6] 邹蕴珏.菖蒲郁金汤治疗围绝经期抑郁症35例[J].中医研究,2007, 20(1):36-37. [7] Zou YJ. Treatment of premenopausal women with depression with Changpu Yujin Tang[J]. Tradit Chin Med Res, 2007, 20(1): 36-37. [8] 卞尧尧,杨丽丽,王中立,等.母婴分离对小鼠行为学及胶质纤维酸性蛋白表达的影响[J].广东医学,2015, 36(15):2309-2311. [9] Bian YY, Yang LL, Wang ZL, et al. Effect of maternal separation on behavior and glial fibrillary acidic protein expression in C57BL/6N Mice[J]. Guangdong Med J, 2015, 36(15): 2309-2311. [10] 戴建国,王中立,陈琳,等.6种宁神类药物抗抑郁作用动物实验研究[J].南京中医药大学学报,2014, 30(2):192-194. [11] Dai JG, Wang ZL, Chen L, et al. Animal experimental study on the antidepressant effects of six tranquilizing medicines[J]. J Nanjing Univ Tradit Chin Med, 2014, 30(2): 192-194. [12] 郭嘉成.菖蒲郁金汤对TS模型小鼠外周血中神经递质及其代谢产物的影响[D].兰州:甘肃中医学院,2014. [13] Guo JC. The Effect of Changpu Yujin Decoction on TS Mouse Models Neurotransmitter in Blood and Its Metabolites[D].Lanzhou:Gansu college of traditional Chinese medicine,2014. [14] 薛付春.菖蒲郁金汤对多发性抽动症模型小鼠脑组织中单胺类神经递质含量的影响[D].兰州:甘肃中医学院,2014. [15] Xue FC.The Effect of Changpu Yujin Tang on the Content of Monoamine Neurotransmitters in Brain tissue of Multiple Tics Mice Model[D].Lanzhou:Gansu college of traditional Chinese medicine,2014. [16] Ma L, Shen CA, Gao L, et al. Anti-inflammatory activity of chitosan nanoparticles carrying NF-κB/p65 antisense oligonucleotide in RAW264.7 macrophage stimulated by lipopolysaccharide[J]. Colloids Surf B Biointerfaces, 2016, 142: 297-306. [17] Hansen H, Bjelland AM, Ronessen M, et al. LitR is a repressor of syp genes and has a temperature-sensitive regulatory effect on biofilm formation and colony morphology in Vibrio (Aliivibrio) salmonicida[J]. Appl Environ Microbiol, 2014, 80(17): 5530-5541. [18] Chmielarz P, Kreiner G, Nalepa I. Selective ablation of glucocorticoid receptors in the noradrenergic system affects evening corticosterone levels in a sex-dependent manner[J]. Pharmacol Rep, 2015, 67(6): 1201-1203. [19] 许委娟,许毅.抑郁症与神经可塑性[J].国际精神病学杂志,2011, 20(3):184-187. [20] Xu WJ, Xu Y. Depression and neural plasticity[J]. J Int Psychl, 2011, 20(3): 184-187.
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