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丹参-人参组分配伍对肺癌A549细胞侵袭迁移及ERK1/2磷酸化水平的影响

毕蕾 颜晓静 杨烨 邱爽 陈卫平

毕蕾, 颜晓静, 杨烨, 邱爽, 陈卫平. 丹参-人参组分配伍对肺癌A549细胞侵袭迁移及ERK1/2磷酸化水平的影响[J]. 南京中医药大学学报, 2016, 32(2): 153-156.
引用本文: 毕蕾, 颜晓静, 杨烨, 邱爽, 陈卫平. 丹参-人参组分配伍对肺癌A549细胞侵袭迁移及ERK1/2磷酸化水平的影响[J]. 南京中医药大学学报, 2016, 32(2): 153-156.
BI Lei, YAN Xiao-jing, YANG Ye, QIU Shuang, CHEN Wei-ping. Effects of Component Formula of Salviae Miltiorrhizae Radix Et Rhizome and Ginseng Panax Et Rhizome on Cell Migration, Invasionand and Phosphorylation of ERK1/2 in Lung Cancer A549 Cells[J]. Journal of Nanjing University of traditional Chinese Medicine, 2016, 32(2): 153-156.
Citation: BI Lei, YAN Xiao-jing, YANG Ye, QIU Shuang, CHEN Wei-ping. Effects of Component Formula of Salviae Miltiorrhizae Radix Et Rhizome and Ginseng Panax Et Rhizome on Cell Migration, Invasionand and Phosphorylation of ERK1/2 in Lung Cancer A549 Cells[J]. Journal of Nanjing University of traditional Chinese Medicine, 2016, 32(2): 153-156.

丹参-人参组分配伍对肺癌A549细胞侵袭迁移及ERK1/2磷酸化水平的影响

Effects of Component Formula of Salviae Miltiorrhizae Radix Et Rhizome and Ginseng Panax Et Rhizome on Cell Migration, Invasionand and Phosphorylation of ERK1/2 in Lung Cancer A549 Cells

  • 摘要: 目的 研究丹参-人参组分配伍对肺癌A549细胞侵袭迁移以及ERK1/2磷酸化水平的影响。方法 采用细胞划痕实验和高内涵细胞成像系统分析丹参-人参组分配伍对肺癌A549细胞迁移的影响;应用实时细胞传感电阻仪Transwell实验动态检测丹参-人参组分配伍对肺癌A549细胞侵袭的影响;通过纳米级超微量蛋白质分析系统检测丹参-人参组分配伍对肺癌A549细胞ERK1/2磷酸化水平的影响。结果 丹参-人参组分配伍可显著增加划痕空白区域的距离(P<0.01),且呈一定的时间依赖性;显著降低细胞迁移的面积(P<0.01),且呈一定的剂量依赖性;对A549细胞侵袭有抑制作用(P<0.01),且呈一定的时间依赖性;能够显著降低肺癌A549细胞ERK1/2磷酸化水平(P<0.01)。结论 丹参-人参组分配伍能显著降低肺癌A549细胞迁移侵袭的能力,其作用机制可能与降低肺癌A549细胞ERK1/2磷酸化水平有关。

     

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出版历程
  • 收稿日期:  2015-11-25
  • 修回日期:  2016-01-15
  • 刊出日期:  2016-03-10

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