开心散配伍比例对慢性压力应激抑郁小鼠皮层与海马神经营养因子系统调控的影响
Research of Compatible Ratio of Kai-Xin-San on Regulation of Neurotrophic Factor System in Cortex and Hippocampus on Chronic Unpredictable Mild Stress Induced Depressive Mice
-
摘要: 目的 基于慢性压力应激抑郁小鼠模型,考察不同配伍比例的开心散对皮层与海马神经营养因子调控的影响,探讨开心散治疗抑郁症的作用机制。方法 采用一系列慢性应激压力构建小鼠抑郁症模型,给予不同配伍比例的开心散,应用ELISA法分别检测小鼠大脑皮层与海马中神经生长因子NGF与脑源性神经营养因子BDNF含量,利用qPCR与Western blot技术检测皮层与海马神经营养因子受体TrkA、TrkB、TrkC的表达。结果 不同配伍比例的开心散均能提升抑郁小鼠的糖水饮用量,延长强迫游泳时间,表现出显著的抗抑郁作用,其中以D-652配伍比例效用最佳。同时提升NGF、BDNF含量与相关受体的表达。结论 开心散调控神经营养因子系统是其抗抑郁的重要作用机制。Abstract: OBJECTIVE To explore the anti-depressive effect of Kai-Xin-San (KXS) with different compatible ratio on chronic unpredictable mild stress (CUMS) treated mouse via neurotrophic factor regulation. METHODS Depressive animal model was created by applying a series of stress procedures on mice and the depressive mice were treated with KXS with different compatible ratio. Several parameters, including sucrose consumption, forced swimming time, were applied for evaluation of the depressive effect of KXS. Afterwards, the mice were sacrificed. The cerebral cortex and hippocampus were took out for the analysis of quantity of neurotrophic factors and the expressions of neurotrophic factor receptors via ELISA, qPCR and western blot analysis. RESULTS KXS with different compatible ratio exerted significant anti-depressive effect on CUMS treated depressive mouse model in presence of increased sucrose consumption, swimming time and open field scores. The amount of neurotrophic factors, NGF and BDNF, and the expression of the corresponding receptors, were both up-regulated after the treatment of KXS. One ratio named D-652 exerted the best effect. CONCLUSION Neurotrophic factor regulation might be one of important active targets for anti-depression effect of KXS.
-
Key words:
- Kai-Xin-San /
- NGF /
- BDNF /
- neurotrophic receptor /
- cortex /
- hippocampus /
- formulae compatibility
-
[1] 李景荣. 备急千金要方校释[M]. 北京:人民卫生出版社,1998:320-321. [2] Li JR. Proof and Explanation about Thousand-Golden-Prescriptions[M]. Beijing:People's medical publishing house,1998:320-321. [3] Zhu KY, Mao QQ, Ip SP, et al. A standardized Chinese herbal decoction, kai-xin-san, restores decreased levels of neurotransmitters and neurotrophic factors in the brain of chronic stress-induced depressive rat[J]. Evid Based Complement Alternat Med. 2012,2012:149256. [4] Zhu KY, Fu Q, Xie HQ, et al. Quality assessment of a formulated Chinese herbal decoction, Kaixinsan, by using rapid resolution liquid chromatography coupled with mass spectrometry: A chemical evaluation of different historical formulae[J]. J Sep Sci, 2010, 33(23/24): 3666-3674. [5] Zhu KY, Xu SL, Choi RC, et al. Kai-Xin-San, a Chinese herbal decoction containing ginseng radix et rhizoma, polygalae radix, acori tatarinowii rhizoma, and poria, stimulates the expression and secretion of neurotrophic factors in cultured astrocytes[J]. Evid Based Complement Alternat Med. 2013,2013: 731385. [6] Marc DB, Nobutaka H, Uwe W. Encyclopedia of Neiroscience[M]. London: Springer, 2009: 2231. [7] Schmidt HD, Banasr M, Duman RS.Future antidepressant targets: Neurotrophic factors and related signaling cascades[J]. Drug Discov Today Ther Strateg, 2008,5(3):151-156. [8] 李国彰.神经生理学[M].北京:人民卫生出版社,2007:223-225. [9] Li GZ.Neurophysiology[M].Beijing:People's medical publishing house,2007:223-225. [10] Schmidt HD, Duman RS. The role of neurotrophic factors in adult hippocampal neurogenesis, antidepressant treatments and animal models of depressive-like behavior[J]. Behav Pharmacol, 2007, 18(5/6): 391-418. [11] Aydemir O, Deveci A, Taneli F. The effect of chronic antidepressant treatment on serum brain-derived neurotrophic factor levels in depressed patients: a preliminary study[J]. Prog Neuropsychopharmacol Biol Psychiatry, 2005, 29(2): 261-265. [12] Okamoto T, Yoshimura R, Ikenouchi-Sugita AA, et al. Efficacy of electroconvulsive therapy is associated with changing blood levels of homovanillic acid and brain-derived neurotrophic factor (BDNF) in refractory depressed patients: A pilot study[J]. Prog Neuropsychopharmacol Biol Psychiatry, 2008, 32(5): 1185-1190. [13] Diniz BS, Teixeira AL, Miranda AS, et al. Circulating glial-derived neurotrophic factor is reduced in late-life depression[J]. J Psychiatr Res, 2012, 46(1): 135-139. [14] Hashimoto K, Shimizu E, Iyo M.Critical role of brain-derived neurotrophic factor in mood disorders[J]. Brain Res Brain Res Rev, 2004, 45(2):104-114. [15] Hisaoka K, Nishida A, Koda T, et al. Antidepressant drug treatments induce glial cell line-derived neurotrophic factor (GDNF) synthesis and release in rat C6 glioblastoma cells[J]. J Neurochem, 2001, 79(1):25-34. [16] 沈澍农.医心方校释[M].北京:学苑出版社,2001:1635. [17] Shen SN. Proof and Explanation about Yixinfang[M].Beijing:The academy press,2001:1635. [18] 包祖晓,赵国平,孙伟,等.开心散治疗轻、中度抑郁症临床观察[J].中华中医药学刊,2011, 28(5):987-988. [19] Bao ZX, Zhao GP, Sun W, et al. Clinical curative effects of Kaixin powder on depression with mild or moderate degree[J]. Chin Arch Tradit Chin Med, 2011, 28(5): 987-988. [20] 刘婉婉,许璐,董宪喆,等.开心散配伍比例对慢性应激大鼠行为学及中枢单胺类神经递质的影响[J].中国中药杂志,2015, 40(11):2180-2185. [21] Liu WW, Xu L, Dong XZ, et al. Effects of kaixin San formulas on behavioristics and central monoamine neurotransmitters of chronic stress rats[J]. China J Chin Mater Medi, 2015, 40(11): 2180-2185. [22] 刘明,闫娟娟,周小江,等.开心散对慢性应激抑郁模型大鼠学习记忆的影响[J].中国中药杂志,2012, 37(16):2439-2443. [23] Liu M, Yan JJ, Zhou XJ, et al. Effect of kaixin San on learning and memory in chronic stress depression model rats[J]. China J Chin Mater Med, 2012, 37(16): 2439-2443.
点击查看大图
计量
- 文章访问数: 787
- HTML全文浏览量: 16
- PDF下载量: 740
- 被引次数: 0