电针对中枢Stat5敲除所致肥胖小鼠瘦素水平和下丘脑Kctd15、Sh2b1 mRNA的影响

洪浩; 卢圣锋; 傅淑平; 偶晨; 朱冰梅

电针对中枢Stat5敲除所致肥胖小鼠瘦素水平和下丘脑Kctd15、Sh2b1 mRNA的影响

南京中医药大学第二临床医学院，江苏南京 210023

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出版历程
- 刊出日期: 2016-01-10

Effects of Electro-acupuncture on Serum Leptin Content and Expression of Kctd15 and Sh2b1 mRNA in Hypothalamus in Stat5-knockout Induced Obese Mice

摘要

摘要: 目的观察电针对中枢Stat5条件性敲除Stat5 Nestin-Cre (Stat5NKO)肥胖小鼠下丘脑Kctd15、Sh2b1基因表达的影响。方法 20只肥胖的Stat5NKO小鼠随机分为肥胖组和电针组，每组10只；10只Stat5fl/fl小鼠作为正常组。其中电针组选取单侧后三里、内庭穴进行针刺治疗，每日1次，双侧交替进行，每次30 min，韩氏电针仪频率2/15 Hz，疏密波，6 d为1个疗程，共治疗4个疗程。运用ELISA法检测血清中瘦素（Leptin）含量，qPCR技术检测下丘脑中肥胖相关基因Kctd15、Sh2b1 mRNA的表达。结果与正常组小鼠比较，肥胖组小鼠体质量明显增加(P<0.01)伴随血清Leptin含量升高(P<0.05)，下丘脑Leptin水平下降(P<0.05)，同时下丘脑Kctd15、Sh2b1 mRNA表达下调(P<0.05~0.01)；与肥胖组小鼠比较，电针组小鼠的体质量明显下降(P<0.01)，血清Leptin含量降低(P<0.01)，且下丘脑Leptin水平回升(P<0.01)，Kctd15、Sh2b1 mRNA表达水平上调(P<0.01)。结论电针可能通过调节Leptin水平并促进Kctd15、Sh2b1表达，实现对Stat5NKO小鼠的减肥效应。
- 肥胖 /
- 电针 /
- 瘦素 /
- Kctd15 /
- Sh2b1
Abstract: OBJECTIVE To observe the effect of electro-acupuncture on central conditional knockout Stat5 Nestin-Cre obese mice (Stat5NKO) and detect how the obesity related genes， Kctd15 and Sh2b1， are involved. METHODS 20 obese Stat5NKO mice were randomly divided into two groups: obese model group (n=10) and electro-acupuncture （EA） group (n=10); 10 Stat5fl/fl mice were randomly selected and fed with ordinary forage as normal control group. EA group was treated with electro-acupuncture at Housanli and Neiting points for 30 min， with bilateral alternates， by Han' electro-acupuncture apparatus at 2/15 Hz. 6 d was a period of treatment， with a total of 4 courses of treatment. Serum Leptin content was measured by ELISA， and the levels of Sh2b1 and Kctd15 mRNA in hypothalamus were detected by qPCR. RESULTS Compared with Stat5fl/fl mice， Stat5NKO mice gained body weight significantly (P<0.01)， accompanied with increased serum Leptin level (P<0.05)， decreased hypothalamic Leptin level (P<0.05)， as well as Kctd15 and Sh2b1 mRNA expressions (P<0.05~0.01); Compared with Stat5NKO mice， EA group decreased body weight (P<0.01) and serum Leptin mRNA level (P<0.01). The hypothalamic Leptin level was elevated (P<0.01)， and the expressions of Kctd15 and Sh2b1 mRNA were returned to normal level (P<0.01). CONCLUSION The weight-loss effect of electro-acupuncture on Stat5NKO obese mice may be related to the regulation of the expression of Leptin， Kctd15 and Sh2b1.
- obesity /
- electro-acupuncture /
- Leptin /
- Kctd15 /
- Sh2b1

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参考文献(9)

[1]	Cho SH， Lee JS， Thabane L， et al. Acupuncture for obesity: a systematic review and meta-analysis[J]. Int J Obes (Lond)， 2009， 33(2): 183-196.
[2]	于月，尤佳，杨柳，等．瘦素信号转导通路在能量代谢平衡中的作用机制[J]．生命科学，2013， 25（2）：169-175．
[3]	Yu Y， You J， Yang L， et al. Leptin receptor signaling in energy homeostasis[J]. Chin Bull Life Sci， 2013， 25(2): 169-175.
[4]	Shen W， Wang Y， Lu SF， et al. Acupuncture promotes white adipose tissue browning by inducing UCP1 expression on DIO mice[J]. BMC Complement Altern Med， 2014， 14: 501.
[5]	Lee JY， Muenzberg H， Gavrilova O， et al. Loss of cytokine-STAT5 signaling in the CNS and pituitary gland alters energy balance and leads to obesity[J]. PLoS One， 2008， 3(2): e1639.
[6]	Myers J， Olson DP. Central nervous system control of metabolism[J]. Nature， 2012， 491(7424): 357-363.
[7]	Zarelli VE， Dawid IB. Inhibition of neural crest formation by Kctd15 involves regulation of transcription factor AP-2[J]. Proc Natl Acad Sci USA， 2013， 110(8): 2870-2875.
[8]	Cao H， Sekiya M， Ertunc ME， et al. Adipocyte lipid chaperone AP2 is a secreted adipokine regulating hepatic glucose production[J]. Cell Metab， 2013， 17(5): 768-778.
[9]	De Lartigue G， Ronveaux CC， Raybould HE. Deletion of leptin signaling in vagal afferent neurons results in hyperphagia and obesity[J]. Mol Metab， 2014， 3(6): 595-607.