电针大肠俞募穴对功能性便秘小鼠结肠组织GDNF表达的影响

张微; 李瑛; 刘丽莎; 罗芳丽; 郑倩华

电针大肠俞募穴对功能性便秘小鼠结肠组织GDNF表达的影响

成都中医药大学针灸推拿学院，四川成都 610072

计量
- 文章访问数: 997
- HTML全文浏览量: 4
- PDF下载量: 1256
- 被引次数: 0
出版历程
- 收稿日期: 2015-01-06
- 修回日期: 2015-05-22
- 刊出日期: 2015-09-10

Effects of Electroacupuncture at Back-shu and Front-mu Points on Expression of GDNF in Colon Tissue of Functional Constipation Mice

摘要

摘要: 目的探讨电针大肠俞募穴治疗功能性便秘的作用机制。方法 32只小鼠随机分为对照组、模型组、针刺组和EGC抑制组。采用复方地芬诺酯灌胃造模法，电针刺激天枢、大肠俞，每次30 min，共治疗5次。运用光镜、电镜、免疫组化及原位杂交技术，观察电针治疗前后结肠组织上皮细胞形态学改变，结肠组织中GDNF蛋白及mRNA表达水平。结果与对照组比较，模型组结肠组织GDNF蛋白及mRNA表达水平显著降低（P<0.01）。电针刺激大肠俞募穴可提高结肠中GDNF蛋白及其mRNA的表达水平（P<0.05），光镜和电镜发现针刺可修复受损上皮细胞，进而改善肠道传输功能。EGC抑制组结肠中GDNF蛋白表达与模型组比较无显著性差异（P>0.05），与针刺组比较显著降低（P<0.05），GDNF mRNA的表达水平高于模型组（P<0.05），低于针刺组，但差异无统计学意义（P>0.05）。光镜和电镜下EGC抑制组结肠组织形态改变较为严重。结论电针刺激大肠俞募穴可提高EGC细胞中GDNF蛋白及其mRNA的表达水平，修复受损结肠上皮细胞，进而改善肠道传输功能。
- 电针 /
- 俞募穴 /
- 功能性便秘 /
- GDNF
Abstract: OBJECTIVE To explore the mechanism of electroacupuncture at Back-shu and Front-mu points for functional constipation treatment. METHODS 32 mice were randomly divided into control group， model group， electroacupuncture (EA) group and EGC group. Compound diphenoxylate were gavaged for modeling， and electroacupuncture Tianshu and Dachangshu points， 30 min each time for a total of 5 times treatment. By light microscopy， electron microscopy， immunohistochemistry and in situ hybridization， the morphology of colonic epithelial structure were observed， and the level of GDNF protein and mRNA expression in colon were detected. RESULTS The expression of GDNF protein and mRNA in model group was significantly decreased (P<0.01). Electroacupuncture at back-Shu and front-Mu points improved the expression of GDNF protein and mRNA (P<0.05)， and light microscope and electron microscope showed that acupuncture repaired damaged depithelial cells， and then improved the intestinal transmission function. The expression of GDNF protein in EGC group showed no significant difference with the model group (P>0.05)， but significantly decreased， compared with EA group (P<0.05). GDNF mRNA was significantly higher than model group (P<0.05)， but lower than EA group with no significant difference (P>0.05). The morphology change of colon tissue is more serious. CONCLUSION Electroacupuncture at back-Shu and front-Mu points can improve the expression of GDNF protein and mRNA in EGC cells， repair the colonic epithelial cells， and then improve the intestinal transmission function.
- electroacupuncture /
- Back-shu and Front-mu points /
- functional constipation /
- GDNF

HTML全文

参考文献(27)

[1]	魏艳静，卞红磊，赵发. 便秘的国内流行病学研究进展[J]. 中华疾病控制杂志， 2004， 8(5):449-451.
[2]	Wei JY， Bian HL， Zhao F. Internal progress of epidemiology study on constipation[J]. Chin J Dis Control Prev， 2004， 8(5):449-451.
[3]	丁美红，林征，王美峰，等.功能性便秘患者症状、精神心理状况、自主神经功能相关性研究[J].护理学报，2010，17(4A):4-7.
[4]	Ding MH， Lin Z， Wang MF， et al. Study on correlation of symptoms， psychological status and autonomic nervous function among patients with functional constipation[J]. J Nurs，2010，17(4A):4-7.
[5]	杜文菲，于璐，严兴科，等.针灸治疗便秘随机对照临床研究文献Meta分析[J].中国针灸，2012，32（1）：92-96.
[6]	Du WF， Yu L， Yan XK， et al. Meta-analysis on randomized controlled clinical trials of acupuncture and moxibustion on constipation[J].Chin Acup Mox，2012，32（1）：92-96.
[7]	邱学梅，杜帅，陈少宗. 针灸治疗便秘取穴规律文献分析[J]. 山东中医药大学学报， 2014， 38(2):113-115.
[8]	Qiu XM， Du S， Chen SZ. Ananlysis of acupoint rules of constipation treated with acupuncture and moxibustion[J]. J Shandong Univ Chin Med， 2014， 38(2):113-115.
[9]	袁媛，范一宏，张烁，等.肠神经胶质细胞及胶质细胞源性神经营养因子在肠道功能中的作用[J].中华内科杂志，2013，52（11）：995-996.
[10]	Yuan Y， Fan YH， Zhang S， et al. The role of enteric glial cells and glial cell derived neurotrophic factors in intestinal function[J].Chin J Intern Med，2013，52（11）：995-996.
[11]	姚景春，冯芹，孙宝存. 己酮可可碱对慢传输型便秘模型大鼠的结肠黏液分泌和结肠肌电活动的影响[J]. 中国药理学通报， 2011， 27(12):1749-1752.
[12]	Yao JC， Feng Q， Sun BC. Effects of pentoxifylline on colonic mucus and colonic electromyography in rats with slow transi constipation[J]. Chin Pharmacol Bull， 2011， 27(12):1749-1752.
[13]	范一宏，徐国萍，冯雯，等. 枳术通便汤对慢传输型便秘大鼠结肠墨汁推进率、GDNF及NOS mRNA表达的影响[J]. 中国中西医结合杂志， 2012， 32(4):486-489.
[14]	Fan YH， Xu GP， Feng W， et al. Effects of Zhizhu Tongbian decoction on the colon ink propelling rate， GDNF and NOS mRNA expression in rats with slow transit constipation[J]. Chin J Integr Med， 2012， 32(4):486-489.
[15]	陈楚淘，严杰，田浩梅，等. 电针天枢配大肠俞、天枢配上巨虚对溃疡性结肠炎模型大鼠血清NO、MDA影响的比较研究[J]. 中医药导报， 2008， 14(12):1-3.
[16]	Chen CT， Yan J， Tian HM， et al. Comparative study of electro-acupuncturing at Tianshu and Dachangshu， Tianshu and Shangjuxu on serum NO and MDA in rats with ulcerative colitis[J]. Guid J Tradit Chin Med Pharm， 2008， 14(12):1-3.
[17]	Nasser Y， Fernandez E， Keenan CM， et al. Role of enteric glia in intestinal physiology: effects of the gliotoxin fluorocitrate on motor and secretory function[J]. Am J Physiol Gastrointest Liver Physiol. 2006， 291(5):G912-G927.
[18]	孔文成，任乐乐. 肠神经胶质细胞——肠黏膜屏障构成家族的新成员[J]. 肠外与肠内营养， 2013， 20(6):371-374.
[19]	Kong WC， Ren LL. Enteric glial cell: new player of in intestinal barreier[J]. Parenter Enter Nutr， 2013， 20(6):371-374.
[20]	Kordower JH， Bjorklund A. Trophic factor gene therapy for Parkinson's disease.[J].Mov Disord， 2013， 28(1):96-109.
[21]	Allen SJ， Watson JJ， Shoemark DK， et al. GDNF， NGF and BDNF as therapeutic options for neurodegeneration[J].Pharmacol Ther， 2013，138(2):155-175.
[22]	Aubé AC; Cabarrocas J; Bauer Jet al. Changes in enteric neurone phenotype and intestinal functions in a transgenic mouse model of enteric glia disruption[J]. Gut， 2006， 55(5):630-637.
[23]	马骏，王彦春，王述菊，等. 电针对阿尔茨海默病模型大鼠海马区神经营养因子及其受体表达的影响[J]. 针灸临床杂志， 2010， 26(7):63-66.
[24]	Ma J， Wang YC， Wang SJ， et al. Effect of electroacupuncture therapy on the expression of neurotrophic factor and receptors in hippocampus of the Alzheimerian rats[J]. J Clin Acup Mox， 2010， 26(7):63-66.
[25]	王彦春，程宇核，马骏，等. 电针对帕金森病模型大鼠GDNF及其功能性受体Ret表达的影响[J]. 中国针灸， 2010， 30(9):739-743.
[26]	Wang YC， Cheng YH， Ma J， et al. Effects of electroacupuncture on the expression of GDNF and Ret in Parkinson's disease model rats[J]. Chin Acup Mox， 2010， 30(9):739-743.
[27]	Sun H， Zhao H， Ma C， et al. Effects of electroacupuncture on depression and the production of glial cell line-derived neurotrophic factor compared with fluoxetine: a randomized controlled pilot study[J]. J Altern Complement Med， 2013， 19(9):733-739.