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三黄煎剂调节Aurora激酶A促进乳腺癌细胞凋亡的实验研究

许岩磊 陈曦琰 陈绪 卞卫和 姚昶 朱小纾 陈佳静

许岩磊, 陈曦琰, 陈绪, 卞卫和, 姚昶, 朱小纾, 陈佳静. 三黄煎剂调节Aurora激酶A促进乳腺癌细胞凋亡的实验研究[J]. 南京中医药大学学报, 2015, 31(5): 469-474.
引用本文: 许岩磊, 陈曦琰, 陈绪, 卞卫和, 姚昶, 朱小纾, 陈佳静. 三黄煎剂调节Aurora激酶A促进乳腺癌细胞凋亡的实验研究[J]. 南京中医药大学学报, 2015, 31(5): 469-474.
XUYan-lei, CHENXi-yan, CHENXu, BIANWei-he, YAOChang, ZHUXiao-shu, CHENJia-jing. San Huang Decoction Promotes Apoptosis of Breast Cancer Cells Through Regulating Aurora Kinase A[J]. Journal of Nanjing University of traditional Chinese Medicine, 2015, 31(5): 469-474.
Citation: XUYan-lei, CHENXi-yan, CHENXu, BIANWei-he, YAOChang, ZHUXiao-shu, CHENJia-jing. San Huang Decoction Promotes Apoptosis of Breast Cancer Cells Through Regulating Aurora Kinase A[J]. Journal of Nanjing University of traditional Chinese Medicine, 2015, 31(5): 469-474.

三黄煎剂调节Aurora激酶A促进乳腺癌细胞凋亡的实验研究

San Huang Decoction Promotes Apoptosis of Breast Cancer Cells Through Regulating Aurora Kinase A

  • 摘要: 目的 探讨三黄煎剂对乳腺癌MCF-7、MDA-MB-231细胞凋亡的影响及Aurora激酶A(Aurora A)蛋白表达及功能的影响,并探讨其内在机制。方法 采用CCK-8法检测三黄煎剂对乳腺癌细胞MCF-7、MDA-MB-231增殖的影响。AnnexinV-FITC/PI法检测MCF-7、MDA-MB-231细胞凋亡率。q-PCR法检测MCF-7、MDA-MB-231细胞Aurora A、p53的mRNA表达水平。Western Blot法检测MCF-7、MDA-MB-231细胞凋亡相关蛋白的表达及Aurora A蛋白的表达。结果 三黄煎剂对MCF-7、MDA-MB-231细胞增殖抑制率呈浓度梯度依赖增长(P<0.05),给药48 h疗效好于24 h(P<0.05),与给药72 h无统计学差异(P>0.05)。三黄煎剂能够诱导MCF-7、MDA-MB-231细胞凋亡,并上调c-PARP、c-Caspase 3、Bax蛋白的表达,下调Bcl-2蛋白的表达,呈浓度梯度依赖。三黄煎剂能够下调Aurora A蛋白及mRNA的表达、上调p53蛋白及mRNA的表达。结论 三黄煎剂能够通过下调Aurora A蛋白及mRNA的表达,抑制Aurora A的生物活性,抑制MCF-7、MDA-MB-231细胞增殖,诱导其凋亡。

     

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出版历程
  • 收稿日期:  2015-05-03
  • 修回日期:  2015-08-11
  • 刊出日期:  2015-09-10

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