HPLC同时测定复方五味含片中葛根素、甘草苷、甘草酸铵、五味子醇甲、五味子甲素、五味子乙素
Simultaneous Determination of Six Components in Compound Wuwei Buccal Tablets by HPLC
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摘要: 目的 建立HPLC同时测定复方五味含片中葛根素、甘草苷、甘草酸铵、五味子醇甲、五味子甲素、五味子乙素6种成分含量的方法。方法 KromasiL C18柱（250 mm×4.6 mm，5 μm）；流动相：乙腈-0.05%磷酸水溶液，梯度洗脱；流速1.0 mL/min；柱温30 ℃，检测波长250 nm。结果 葛根素、甘草苷、甘草酸铵、五味子醇甲、五味子甲素、五味子乙素分别在0.031 0~0.154 9、0.013 7~0.068 5、0.042 4~0.211 8、0.017 4~0.087 1、0.001 8~0.009 1、0.013 6~0.068 1 mg/mL线性范围良好；相关系数均大于0.999 5；平均加样回收率分别为98.6%、100.4%、98.3%、98.3%、96.8%、99.4%；RSD分别为2.6%、2.8%、1.2%、2.3%、0.9%、2.5%。结论 该方法简便、重复性好，可用于复方五味含片的质量控制。Abstract: OBJECTIVE To establish a method to simultaneously determine puerarin， liquiritin， ammonium glycyrrhetate， schizandrol， deoxyschisandrin， and schizandrin B in compound wuwei tablet by HPLC. METHODS The elusion was performed on Kromasil C18(250 mm×4.6 mm， 5 μm） column. The mobile phase was composed of acetonitrile and 0.05% H3PO4 with gradient elution. The detection wave length was 250 nm， the flow rate was 1.0 mL/min and the column temperature was maintained at 30 ℃. RESULTS Puerarin， liquiritin， ammonium glycyrrhetate， schizandrol， deoxyschisandrin， schizandrin B are all linear in the range of 0.031 0~0.154 9，0.013 7~0.068 5，0.042 4~0.211 8，0.017 4~0.087 1，0.001 8~0.009 1，0.013 6~0.068 1 mg/mL respectively. Their average recoveries were 98.6%，100.4%，98.3%，98.3%，96.8%，99.4% and RSD of the above six components were 2.6%，2.8%，1.2%，2.3%，0.9%，2.5%. CONCLUSION The method is easy to perform and accurate and practical so that it can be used to determine the six components in the compound wuwei buccal tablets.
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Key words:
- compound wuwei buccal tablet /
- puerarin /
- liquiritin /
- ammonium glycyrrhetate /
- schizandrol /
- deoxyschisandrin /
- schizandrin B
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