益肾化瘀法对成纤维细胞增殖的影响及机制的研究

殷燕云; 凌云; 卞尧尧; 周春祥

益肾化瘀法对成纤维细胞增殖的影响及机制的研究

殷燕云^1,2,
凌云²,
卞尧尧³,
周春祥^2,4,

1.
南京中医药大学附属医院，江苏南京 210029
2.
南京中医药大学伤寒金匮教研室，江苏南京 210023
3.
南京中医药大学护理学院，江苏南京 210023
4.
南京中医药大学明基教学医院，江苏南京 201136

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出版历程
- 刊出日期: 2014-01-10

Study on the Inhibitory Effect of Yishen Huayu Method in the Mechanism of Fibroblast Proliferation

1.
The Affiliated Hospital of Nanjing University of Chinese Medicine, 210029
2.
Golden Chamber of Teaching and Research Office of Nanjing University of Chinese Medicine, Nanjing, 210023, China
3.
Preclinical Medicine College, Nanjing University of Chinese Medicine, 210023
4.
Benq Hospital, Nanjing University of Chinese Medicine, 210036

摘要

摘要: 目的探讨益肾化瘀法对慢性盆腔炎过程中成纤维细胞增殖的干预机制。方法分别以不同浓度的益肾化瘀方、益肾方、化瘀方作用于体外培养的NIH/3T3细胞，以MTT法检测细胞增殖。并观察不同药方对bFGF、TGF-β、HOP表达的影响。结果益肾化瘀方有效抑制成纤维细胞增殖及其相关细胞因子的表达，并减少HOP的产生，呈剂量依赖关系。而拆方组，益肾方与益肾化瘀方相比，其抑制成纤维细胞增殖及相关机制的效应显著较低，P<0.05；与之相较，化瘀方与益肾化瘀方相比，其差异也具有显著性，P<0.05，但比益肾方抑制成纤维细胞增殖的效应佳。结论益肾化瘀法具有显著抑制慢性盆腔炎过程中成纤维细胞增殖的效应，其机制是通过抑制成纤维细胞增殖相关细胞因子的表达及抑制细胞外基质相关胶原蛋白的表达实现的。与益肾法、化瘀法相比，复法合方的效应最优，差异显著。
- 益肾化瘀 /
- 慢性盆腔炎 /
- 成纤维细胞 /
- 增殖
Abstract: OBJECTIVE To study the effect of Yishen Huayun method on the intervention mechanism of fibroblast proliferation of chronic pelvic inflammatory process. METHODS Different concentrations of YSHY， YS， HY were used in the incubated NIH/3T3 cells and MTT method was used to detect the cell proliferation. And the effect of different prescriptions for bFGF、TGF-β、HOP expression were observed. RESULTS YISHEN Huayun effectively inhibit the expression of fibroblast proliferation and cytokine， and reduce the production of HOP， which is in a dose-dependent manner.Compared with Yishen Huayu method， the inhibitory effect of Yishen method is relatively low; compared with Yinshen Huayu method， Huayu method achieved low effects， but is more useful than Yishen method. CONCLUSION Yishen Huayu method is effective in inhibiting the fibroblast proliferation in treating chronic pelvic inflammatory disease by inhibiting the expression of cytokines related to fibroblast proliferation and the expression of certain collagen in the extracellular matrix. Compared with the Yinshen method and Huayu method， the combined formula can achieve the best effect.
- Yishen Huayu method /
- Chronic pelvic inflammatory disease /
- fibroblast /
- proliferation

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参考文献(6)

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