UPLC法测定宣木瓜中齐墩果酸、熊果酸
Detecting Oleanolic and Ursolic Acid with UPLC in Chaenomelis Fructus from Xuancheng
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摘要: 目的 对高效液相色谱法（HPLC）进行转换并优化，建立超高效液相色谱法(UPLC)测定宣木瓜中齐墩果酸（OA）和熊果酸(UA)含量，并与HPLC测定法相比较。方法 UPLC采用ACQUITY UPLCTM BEH C18色谱柱(100 mm×2.1 mm， 1.7 μm)，流动相：甲醇-水-冰乙酸-三乙胺（254∶46∶0.1∶0.05）；流速：0.3 mL/min，检测波长：210 nm，柱温：25 ℃，进样体积：1 μL；HPLC色谱柱为Shim-pack CLC-ODS(M) (250 mm×4.6 mm，5 μm)，流动相：甲醇-水-冰乙酸-三乙胺（265∶35∶0.10∶0.05）；流速：0.6 mL/min；柱温及检测波长同UPLC；进样体积：20 μL。结果 2种方法测定不同采收期宣木瓜中OA和UA含量，结果基本一致；UPLC法分析时间短，溶剂消耗少，检测灵敏度高。结论 UPLC法可替代HPLC法测定宣木瓜药材中OA和UA含量。Abstract: OBJECTIVE To transform and develop high performance liquid chromatograph(HPLC)， to establish ultra performance liquid chromatography (UPLC) for determinating the contents of oleanolicacid (OA) and ursolicacid (UA) in Chaenomelis Fructus and to compare UPLC with HPLC.METHODS In the UPLC， ACQUITY UPLC TM BEHC18 column(100 mm×2.1 mm， 1.7 μm) was adopted， and the column temperature was 25℃.The mobile phase consisted of methanol-water-glacial acetic acid-triethylamine (254∶46∶0.1∶0.05) with a flow rate of 0.3mL/min.The detection wave length was 210nm， and injection volume was 1 μL in UPLC system. A Shim-pack CLC-ODS(M) column (250mm×4.6mm，5μm) was utilized in HPLC system， and a mixture of methanol-water-glacial acetic acid-triethylamine (265∶35∶0.1∶0.05) was regarded as mobile phase with a flow rate of 0.6mL/min.The Injection volume of HPLC was 20μL.RESULTS The content of oleanolicacid (OA) and ursolicacid (UA) in Chaenomelis Fructus of different picking time detected by HPLC was almost as same as that by UPLC. Compared with HPLC， UPLC provided shorter analysis time， cost less solvent consumption with better sensitivity.CONCLUSION UPLC may replace HPLC for the determination of OA and UA in Chaenomelis Fructus.
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Key words:
- UPLC /
- HPLC /
- chaenomelis fructus /
- oleanolic acid /
- ursolic acid
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[1] 黄立厚，王尚全，朱永芳．宣木瓜品种性状的调查[J]．中药材，1986，9（6）：1-5． [2] Huang LH， Wang SQ， Zhu YF. Investigation of characters for cultivated varieties of chaenomelis fructus from xuancheng[J].Chin Med Mat，1986，9(6):1-5. [3] 吕美红，谢晓梅，查孝柱，等．反相高效液相色谱法测定木瓜药材及饮片中齐墩果酸和熊果酸含量[J]．安徽中医学院学报，2010，29（3）：72-75． [4] Lv MG， Xie XM， Zha XZ， et al. Determination of oleanolic acid and ursolic acid in the medicinal materials and prepared slices of fructus chaenomelis by reversed Phase-High performance liquid chromatography[J].J Anhui TCM College，2010，29(3):72-75. [5] 周新，陈会明，白桦，等．HPLC与UPLC色谱条件转换方法研究[J]．分析试验室，2008， 27（4）：56-58． [6] Zhou X， Chen HM， Bai H， et al. Study on conversion of chromatographic process between HPLC and UPLC[J]. Chin J Anal Lab， 2008， 27(4): 56-58. -
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