寒凝血瘀证大鼠模型的优化筛选研究

谢波; 邓海山; 喻斌; 方泰惠

寒凝血瘀证大鼠模型的优化筛选研究

南京中医药大学江苏省中药药效与安全性评价重点实验室，江苏南京 210023

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出版历程
- 收稿日期: 2013-03-24
- 修回日期: 2013-04-12
- 刊出日期: 2013-05-10

Study on optimal screening of rat model with blood stasis due to cold syndrome

摘要

摘要: 目的探讨不同冷冻时间对寒凝血瘀证动物模型建立的影响，从而为中医学寒凝血瘀证的研究提供实验模型。方法将大鼠随机分5组，即空白组、5 d组、10 d组、15 d组、20 d组。除空白组外，其余大鼠置于0 ℃冰水中15 min，每日1次，各组分别冷冻相应天数，造成寒凝血瘀模型。观察血常规、卵巢和子宫指数和血液流变指标。结果与空白组比较，造模5 d和10 d组大鼠红细胞可见显著降低和APTT的显著延长（P<0.01）；另外造模5天还可见血浆黏度、低转速全血黏度和红细胞聚集指数的显著降低，红细胞变形能力的显著增加，血红蛋白显著降低，子宫指数可见显著减少（P<0.05~0.01）。造模10 d组还可见中性粒细胞百分比的显著增加，TT的显著缩短（P<0.05~0.01）。造模10 d和15 d虽然均可见红细胞压积的显著减少，但是造模10 d组却可见红细胞变形指数的降低，而造模15 d却见其变形指数的显著增加（与空白组比较，P<0.01），TT的显著缩短（P<0.05），血浆黏度的显著增加（P<0.01），另外APTT也有缩短的趋势。而当造模时间延长到20 d，反而可见PT和APTT的显著延长（P<0.01）。但是各组大鼠肛温在整个造模过程中未见显著改变（与空白组比较，P>0.05）。结论造模10 d和15 d可见大鼠出现凝血功能增强，并且15 d组血液流变学异常最明显。该造模方法为中医寒凝血瘀证的研究提供新的思路。
- 寒凝血瘀 /
- 动物模型 /
- 证
Abstract: OBJECTIVE To investigate the effects of freezing time on setting up a rat model of blood stasis due to cold and to provide an experimental model for the study of blood stasis due to cold syndrome with Chinese medicine.METHODS The rats were divided randomly into 5 groups， namely control group， 5-day group， 10-day group， 15-day group and 20-day group. Except those in control group， the rest rats in other groups were induced blood stasis due to cold syndrome by kept in 0 ℃ ice water lasting for 15 mins one time a day. The complete blood count indexes， ovary index， uterus index and blood rheology were carefully observed.RESULTS Compared with control group， the erythrocytes of rats in 5-day and 10-day group were dramatically reduced， the APTT dramatically extended (P<0.01). In 5-day group， plasma viscosity， blood viscosity and erythrocyte aggregation index (EAI) reduced dramatically， erythrocyte deformability improved significantly， hemoglobin decreased remarkably， and uterus index declined significantly (P<0.05~0.01).The 10-day group also showed that the percentage of neutrophils increased significantly and TT shortened remarkably (P<0.05~0.01). In 10-day and 15-day group， both of their HCT reduced obviously. HBX of 10-day group declined， while that of 15-day group increased significantly (compared with the control group， P<0.01). Besides， TT obviously shortened (P<0.05)， plasma viscosity increased significantly (P<0.01)， and APTT also tended to be short in 15-day group. When frozen time lasted for 20days， PT and APTT were prolonged significantly (P<0.01).But rectal temperature of rats in each group had no significant change during the whole modeling process (compared with control group， P>0.05).CONCLUSION Making model on the 10^th and 15^th day of modeling， coagulative function improved， and abnormal hemorheology was the most obvious in 15-day group compared with other groups. The modeling approach may provide new ideas for the research of blood stasis due to cold syndrome.
- blood stasis due to cold syndrome /
- animal model /
- syndrome

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参考文献(15)

[1]	谷万里，张俏，史载祥．寒凝血瘀证动物模型的研究述评[J]．中国中医药信息杂志，2007， 14（6）：89-91．
[2]	Gu ML， Zhang Q， Shi ZX.Review on the study of animal model with blood stasis due to cold syndrome[J]. Chin J Inf Tradit Chin Med， 2007， 14(6): 89-91.
[3]	杜惠兰，柴丽娜，哈荔田，等．月经病寒凝血瘀证的病理机制初探[J]．中医杂志，1993， 34（7）：428-430．
[4]	Du HL，Chai LN， Ha LT， et al. Discussion on pathological mechanism of blood stasis due to cold syndrome in menstrual disease[J]. J Tradit Chin Med， 1993， 34(7): 428-430.
[5]	成秀梅，杜惠兰，李丹．寒凝血瘀证动物模型的创建[J]．中国中医基础医学杂志，2005， 11（8）：604-605．
[6]	Cheng XM， Du HL， Li D. Establishment of syndrome model of blood stasis due to cold accumulation in rats[J]. Chin J Basic Med Tradit Chin Med， 2005，11(8):604-605.
[7]	李楚杰．冻伤研究的成就[J]．中国病理生理杂志，1990， 6（5）：349-349．
[8]	Li CJ.Research achievements of frostbite[J]. Chin J Pathophysiol， 1990， 6(5): 349-349.
[9]	魏文汉．病理生理学(上册) [M]．上海：上海科学技术出版社，1984：467-569．
[10]	Wei WH. Pathophysiology (first volume)[M]. Shanghai: Shanghai Scientific and Technical Publishers，1984:467-569.
[11]	李恒谋．血液流变学在血瘀证和活血化瘀研究中的作用[J]．实用中西医结合杂志，1992（1）：57．
[12]	Li HM.Hemorheology on the study of blood stasis and promoting blood circulation and removing blood stasis therapy[J]. Pract J Integr Chin Modern Med， 1992(1): 57.
[13]	Marcus ML. Humaral control of coronary circulation.In:Marcus[C]//M， 1983: 177.
[14]	卢阿娜，蔡妍阳，宋梦，等．原发性痛经寒凝血瘀证药效评价模型的建立[J]．中国药理学通报，2010， 26（9）：1242-1245．
[15]	Lu AN， Cai YY， Song M， et al. A pharmacodynamic model for cold-painevaluation in rats with primary dysmenorrhea[J]. Chin Pharmacol Bull， 2010， 26(9): 1242-1245.