抗心衰颗粒对舒张性心衰大鼠RAAS及心肌细胞内TGF-β1的影响
Effect of Anti-heart-failure Granule on RAAS and TGF-β1 in of Rats with Diastolic Heart Failure
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摘要: 目的 观察抗心衰颗粒对舒张性心衰大鼠血浆肾素活性(PRA)、血管紧张素Ⅱ(AT-Ⅱ)、醛固酮(ALD)及转化生长因子β1(TGF-β1)的影响。方法 用腹主动脉缩窄法的方法建立舒张性心衰大鼠模型，随机分为模型组，阳性药组，抗心衰颗粒大、中、小剂量组，另设假手术组，检测各组大鼠血浆ALD、PRA、AT-Ⅱ的水平，并分别用免疫荧光染色法和Western blot法检测其心肌细胞内TGF-β1的表达。结果 贝那普利组与抗心衰颗粒大剂量组AT-Ⅱ低于模型组(P<0.05)，贝那普利组及抗心衰颗粒中、大剂量组ALD较模型组降低(P<0.05)，激光共聚焦观测贝那普利组及抗心衰颗粒各组TGF-β1平均荧光强度均低于模型组(P<0.05)，Western blot法测TGF-β1表达贝那普利组及抗心衰颗粒大剂量组低于模型组(P<0.05)。结论 抗心衰颗粒能显著降低舒张性心衰大鼠的AT-Ⅱ、ALD水平，减少细胞内TGF-β1的表达。Abstract: OBJECTIVE To observe the effect of anti-heart-failure granule on plasma renin activity(PRA)， angiotensinⅡ(AT-Ⅱ)， aldosterone (ALD) and transforming growth factorβ1(TGF-β1) of rats with diastolic heart failure. METHODS With Abdominal aorta constriction to establish model of diastolic heart failure rat， rats were randomly divided into six groups， namely model group， benazepril group， large， medium and small dose of anti-heart-failure granule groups as well as sham-operation group. PRA， AT-Ⅱand ALD of the rats in different groups were tested. The expression of TGF-β1 in cardiomyocytes was carefully detected respectively by immunofluorescence staining techniques and Western-Blot analysis.RESULTS Compared with the model group， AT-Ⅱof benazepril group and anti-heart-failure Granule large dose group decreased(P<0.05); ALD of benazepril goup and anti-heart-faiure Granule large， medium groups decreased(P<0.05). After detection by immunofluorescence staining techniques， it indicated that TGF-β1 of Benazepril group and Anti-heart-failure Granules groups were lower than model group(P<0.05)， while after Western-Blot analysis， it showed that the expression of TGF-β1 of Benazepril group and Anti-heart-failure Granules large dose group was suppressed compared with the model group (P<0.05).CONCLUSION Anti-heart-failure granule may reduce AT-Ⅱand ALD of rats with diastolic heart failure and also may suppress the expression of TGF-β1 in cardiomyocytes.
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Key words:
- Anti-heart-failure Granules /
- DHF /
- AT-Ⅱ /
- ALD /
- TGF-β1
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