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黄芪对高糖腹透液诱导大鼠腹膜间皮细胞EMT中TGF-β1/Smads信号通路的影响

李正红 盛梅笑 张旭 张露 朱长乐 杨劲松

李正红, 盛梅笑, 张旭, 张露, 朱长乐, 杨劲松. 黄芪对高糖腹透液诱导大鼠腹膜间皮细胞EMT中TGF-β1/Smads信号通路的影响[J]. 南京中医药大学学报, 2013, 29(3): 227-232.
引用本文: 李正红, 盛梅笑, 张旭, 张露, 朱长乐, 杨劲松. 黄芪对高糖腹透液诱导大鼠腹膜间皮细胞EMT中TGF-β1/Smads信号通路的影响[J]. 南京中医药大学学报, 2013, 29(3): 227-232.
LIZheng-hong, SHENGMei-xiao, ZHANGXu, ZHANGLu, ZHUChang-le, YANGJin-song. Effect of Radix Astragali on TGF-β1/Smads Signaling Pathway in Epithelial-mesenchymal Transition of rat Peritoneal Mesothelial Cells Induced by High-glucose Peritoneal Dialysate[J]. Journal of Nanjing University of traditional Chinese Medicine, 2013, 29(3): 227-232.
Citation: LIZheng-hong, SHENGMei-xiao, ZHANGXu, ZHANGLu, ZHUChang-le, YANGJin-song. Effect of Radix Astragali on TGF-β1/Smads Signaling Pathway in Epithelial-mesenchymal Transition of rat Peritoneal Mesothelial Cells Induced by High-glucose Peritoneal Dialysate[J]. Journal of Nanjing University of traditional Chinese Medicine, 2013, 29(3): 227-232.

黄芪对高糖腹透液诱导大鼠腹膜间皮细胞EMT中TGF-β1/Smads信号通路的影响

Effect of Radix Astragali on TGF-β1/Smads Signaling Pathway in Epithelial-mesenchymal Transition of rat Peritoneal Mesothelial Cells Induced by High-glucose Peritoneal Dialysate

  • 摘要: 目的 观察黄芪对高糖腹透液诱导大鼠腹膜间皮细胞EMT中TGF-β1/Smads信号蛋白的影响,探讨黄芪对腹膜纤维化的阻抑作用与机制。方法 实验分为5组,每组10只。阴性对照组:每日腹腔注射生理盐水20 mL/只,共35 d;模型组:每日腹腔注射4.25%葡萄糖腹透液20 mL/只,共35 d;黄芪低、中、高剂量组:于造模第16天开始每日腹腔注射黄芪腹透液(腹透液中加入黄芪注射液,分别含黄芪生药终浓度10、20、40 mg/mL)20 mL/只,共20 d。HE染色观察腹膜病理改变;快速免疫组化法检测腹膜组织TGF-β1、CollagenⅠ、Collagen Ⅲ、FN、E-cadherin、α-SMA的表达;Western blot检测腹膜组织TGF-β1、E-cadherin、α-SMA、Smad2/3、p-smad2/3、Smad7蛋白的表达;RT-PCR检测TGF-β1、E-cadherin、α-SMA、Smad7mRNA表达。结果 ①模型组PMCs呈圆形或柱形,有脱落,间皮下基质增生,大量成纤维样细胞及单核、巨噬细胞浸润,纤维素样物质沉积,腹膜组织TGF-β1、CollagenⅠ、Collagen Ⅲ、FN表达上调,黄芪各干预组上述变化有改善,以高剂量组作用较为明显;②模型组EMT标记蛋白α-SMA表达上调,E-cadherin表达下调,高、中剂量黄芪组能一定程度地逆转腹膜组织E-cadherin表达下降与α-SMA高表达;③模型组Samds信号蛋白p-smad2/3、Smad7表达上调,黄芪可不同程度地下调p-smad2/3高表达,增强Samd7的表达,高剂量组作用较为显著。结论 高糖腹透析液可促使PMCs EMT的发生,黄芪很可能通过抑制TGF-β1,并作用于Smads信号转导通路正、负反馈环路中的Smad2/3、Smad7关键信号蛋白,阻抑PMCs EMT的发生。

     

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出版历程
  • 收稿日期:  2013-03-01
  • 修回日期:  2013-04-09
  • 刊出日期:  2013-05-10

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