二至丸抗大鼠肾细胞衰老的GC-MS代谢组学研究
Metabolomics Study of Erzhiwan Against Normal Rat Kidney Cell Senescence Based on GC-MS
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摘要: 目的 研究D-半乳糖诱导的衰老大鼠肾细胞(NRK)内源性代谢物的变化,寻找与衰老有关的代谢生物标志物,探讨二至丸对衰老模型的调节作用及其抗衰老的可能机制。方法 采用D-半乳糖复制NRK细胞衰老模型,借助气相色谱-质谱联用(GC-MS)技术,对各组细胞样本进行测定,采用Kruskal-Wallis非参数检验及Dunn's多重比较进行单变量统计分析来筛选差异性代谢物。结果 经PLS-DA模式识别分析,对照组、模型组和EZW组细胞样本能够得到很好的区分,共鉴定出31种潜在生物标志物,二至丸含药血清给药后衰老NRK细胞胞内的内源性代谢物水平发生不同程度的回调。结论 二至丸可以使D-半乳糖诱导的衰老NRK细胞胞内的异常代谢有所恢复,其治疗作用可能与机体内31个代谢物及10条相关代谢通路的调节有关。Abstract: OBJECTIVE To investigate the changes of endogenous metabolites in senile rat kidney cells (NRK) induced by D-galactose, to find metabolic biomarkers related to aging and to explore the regulation of Erzhiwan on aging model and the anti-aging effect of Erzhiwan. METHODS The senescence model was constructed by D-galactose (D-gal). The gas chromatography-mass spectrometry (GC-MS) technique was used to determine the cell samples in each group. Univariate statistical analysis was performed using the Kruskal-Wallis nonparametric test and Dunn's multiple comparison. RESULTS PLS-DA pattern recognition analysis showed that the cell samples of the control group, the model group and the EZW group could be well distinguished. A total of 31 potential biomarkers were identified, and the endogenous metabolite levels of the senescent NRK cells were varying degrees of callbacks after the administration of the drug-containing serum. CONCLUSION Erzhiwan could restore the abnormal metabolism of D-galactose-induced senescent NRK cells, and its therapeutic effect may be related to the regulation of 31 metabolites and 10 related metabolic pathways.
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Key words:
- senescence /
- Erzhiwan /
- cell metabolomics /
- GC-MS /
- normal rat kidney cell /
- drug-containing serum
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