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泽泻醇B抗4T1乳腺癌细胞侵袭转移的体外研究

张爱凤 盛玉青 邹明畅

张爱凤, 盛玉青, 邹明畅. 泽泻醇B抗4T1乳腺癌细胞侵袭转移的体外研究[J]. 南京中医药大学学报, 2018, 34(2): 178-180.
引用本文: 张爱凤, 盛玉青, 邹明畅. 泽泻醇B抗4T1乳腺癌细胞侵袭转移的体外研究[J]. 南京中医药大学学报, 2018, 34(2): 178-180.
ZHANGAi-feng, SHENGYu-qing, ZOUMing-chang. Investigation on the Anti-Metastatic Effect of Alisol B Isolated from Alismaorientale on 4T1 Breast Cancer Cells in Vitro[J]. Journal of Nanjing University of traditional Chinese Medicine, 2018, 34(2): 178-180.
Citation: ZHANGAi-feng, SHENGYu-qing, ZOUMing-chang. Investigation on the Anti-Metastatic Effect of Alisol B Isolated from Alismaorientale on 4T1 Breast Cancer Cells in Vitro[J]. Journal of Nanjing University of traditional Chinese Medicine, 2018, 34(2): 178-180.

泽泻醇B抗4T1乳腺癌细胞侵袭转移的体外研究

Investigation on the Anti-Metastatic Effect of Alisol B Isolated from Alismaorientale on 4T1 Breast Cancer Cells in Vitro

  • 摘要: 探讨泽泻醇B(Alisol B)对乳腺癌细胞株4T1转移能力的抑制作用及可能机制。方法 4T1细胞经药物作用24 h后,通过MTT法检测药物对细胞生长的影响;通过划痕试验及Transwell小室检测泽泻醇B对细胞侵袭和迁移能力的影响;通过Western blot 检测细胞经药物作用后对基质金属蛋白酶MMP-2及MMP-9蛋白表达水平的变化。结果 泽泻醇B对4T1细胞的生长具有一定的抑制作用。划痕实验显示泽泻醇B可显著抑制乳腺癌细胞的转移能力,细胞经药物(10 μmol/L)作用24 h后,其相对迁移率降至(52.66±8.48)%(P<0.01)。Transwell小室侵袭实验同样证实泽泻醇B能够有效抑制乳腺癌细胞的转移,经药物(10 μmol/L)作用24 h后,24 h内细胞相对侵袭力下降至(47.06±9.32)%(P<0.01)。Western blot结果表明,泽泻醇B能够使癌细胞内MMP-2及MMP-9蛋白表达量显著下调。结论 泽泻醇B在体外能够有效抑制4T1乳腺癌细胞的转移,其机制可能与抑制细胞内基质金属蛋白酶的表达量有关。

     

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出版历程
  • 收稿日期:  2017-09-11
  • 刊出日期:  2018-03-10

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