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骨痹方对大鼠膝骨关节炎PI3K/AKt信号通路及组织形态学的影响

何晓瑾 王磊 王瑞瑞 邱亦江

何晓瑾, 王磊, 王瑞瑞, 邱亦江. 骨痹方对大鼠膝骨关节炎PI3K/AKt信号通路及组织形态学的影响[J]. 南京中医药大学学报, 2018, 34(2): 157-161.
引用本文: 何晓瑾, 王磊, 王瑞瑞, 邱亦江. 骨痹方对大鼠膝骨关节炎PI3K/AKt信号通路及组织形态学的影响[J]. 南京中医药大学学报, 2018, 34(2): 157-161.
HEXiao-jin, WANGLei, WANGRui-rui, QIUYi-jiang. Effects of Gubi Recipe on the PI3K/AKt Pathway and Synovial Tissue of Knee Joint in the Rats Model of Knee Osteoarthritis[J]. Journal of Nanjing University of traditional Chinese Medicine, 2018, 34(2): 157-161.
Citation: HEXiao-jin, WANGLei, WANGRui-rui, QIUYi-jiang. Effects of Gubi Recipe on the PI3K/AKt Pathway and Synovial Tissue of Knee Joint in the Rats Model of Knee Osteoarthritis[J]. Journal of Nanjing University of traditional Chinese Medicine, 2018, 34(2): 157-161.

骨痹方对大鼠膝骨关节炎PI3K/AKt信号通路及组织形态学的影响

Effects of Gubi Recipe on the PI3K/AKt Pathway and Synovial Tissue of Knee Joint in the Rats Model of Knee Osteoarthritis

  • 摘要: 探讨骨痹方对KOA大鼠软骨细胞PI3K/AKt信号通路及膝关节滑膜病理组织形态学的影响。方法 按照改良Hulth's法复制40只KOA大鼠模型随机分为5组:模型组、阳性药组(维固力组)、骨痹方高、中、低剂量组,每组8只,另假手术组(n=8)大鼠术中暴露前后交叉韧带后即可,正常组(n=8)大鼠无需任何处置。每日灌胃1次,共8周。8周后取各组大鼠患膝滑膜,观察其组织形态学变化;应用Western blot法检测膝关节软骨细胞PI3K/AKt信号通路AKt及其下游靶蛋白Bcl-2、Bax的表达。结果 模型组大鼠膝关节滑膜组织形态学检查评分比正常组显著增高(P<0.01);骨痹方中、高剂量组大鼠膝关节滑膜损伤评分较模型组均有所下降(P<0.05)。模型组大鼠AKt及下游靶蛋白Bcl-2表达低于正常组,骨痹方高剂量组AKt表达量显著高于正常组(P<0.01),骨痹方中剂量组AKt表达量与正常组接近;与模型组比较,骨痹方高剂量组AKt表达量及下游靶蛋白Bcl-2显著升高(P<0.01),Bax表达降低。结论 骨痹方可以有效地减少膝骨关节炎病变过程中软骨细胞的凋亡,一定程度地改善膝骨关节炎病理组织形态学,其作用机制可能与其干预KOA大鼠软骨细胞PI3K/AKt信号通路密切有关。

     

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出版历程
  • 收稿日期:  2017-10-11
  • 刊出日期:  2018-03-10

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