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运脾柔肝方对便秘型肠易激综合征大鼠5-HT、VIP及SP影响的实验研究

孙建梅 顾立梅 李慧 华秋雯 田耀洲

孙建梅, 顾立梅, 李慧, 华秋雯, 田耀洲. 运脾柔肝方对便秘型肠易激综合征大鼠5-HT、VIP及SP影响的实验研究[J]. 南京中医药大学学报, 2017, 33(5): 524-528.
引用本文: 孙建梅, 顾立梅, 李慧, 华秋雯, 田耀洲. 运脾柔肝方对便秘型肠易激综合征大鼠5-HT、VIP及SP影响的实验研究[J]. 南京中医药大学学报, 2017, 33(5): 524-528.
SUNJian-mei, GULi-mei, LIHui, HUAQiu-wen, TIANYao-zhou. Effects of Experimental Studies Yunpi Rougan Fang on 5-HT, VIP and SP in Constipation-predominant Irritable Bowel Syndrome Rats[J]. Journal of Nanjing University of traditional Chinese Medicine, 2017, 33(5): 524-528.
Citation: SUNJian-mei, GULi-mei, LIHui, HUAQiu-wen, TIANYao-zhou. Effects of Experimental Studies Yunpi Rougan Fang on 5-HT, VIP and SP in Constipation-predominant Irritable Bowel Syndrome Rats[J]. Journal of Nanjing University of traditional Chinese Medicine, 2017, 33(5): 524-528.

运脾柔肝方对便秘型肠易激综合征大鼠5-HT、VIP及SP影响的实验研究

Effects of Experimental Studies Yunpi Rougan Fang on 5-HT, VIP and SP in Constipation-predominant Irritable Bowel Syndrome Rats

  • 摘要: 目的 通过观察运脾柔肝方对便秘型肠易激综合征(C-IBS)模型大鼠血清、肠组织5-羟色胺(5-HT)、血管活性肠肽(VIP)、P物质(SP)的影响,探讨运脾柔肝方对C-IBS模型大鼠的可能作用机制。方法 将42只Wistar大鼠,随机选取6只作为正常组,剩余36只大鼠采用冰水灌胃法造模14 d,评价模型,随机选取造模成功的30只大鼠,采用随机数字法分为运脾柔肝方中药低、中、高剂量组、莫沙必利组、模型对照组,每组6只。运脾柔肝方低、中、高剂量组分别以12.2、24.4、48.8 g/(kg·d)的给药剂量灌胃,莫沙必利组以莫沙必利混悬液1.35 mg/(kg·d)灌胃,模型对照组及空白组给予等量蒸馏水灌胃。14 d后观察各组大鼠一般情况,并检测各组大鼠血清5-HT、VIP、SP水平和肠组织5-HT、VIP的含量。结果 与空白组相比,模型对照组血清、结肠组织免疫组显5-HT、VIP的表达水平较正常组显著升高(P<0.01),而血清SP的表达水平则明显降低(P<0.05),不同剂量运脾柔肝方均可显著降低C-IBS模型大鼠血清5-HT、VIP的表达水平(P<0.01),明显升高大鼠血清SP水平(P<0.05),免疫组化结果显示各组均能明显降低5-HT在肠组织中的表达(P<0.05),显著降低VIP的表达(P<0.01)。结论 运脾柔肝方可显著改善C-IBS模型大鼠便秘症状,可能是通过降低5-HT、VIP和升高SP的表达水平,从而调节肠道功能,发挥治疗C-IBS的作用。

     

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出版历程
  • 收稿日期:  2017-03-11
  • 修回日期:  2017-07-22
  • 刊出日期:  2017-09-10

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