基于GC-MS代谢组学的RSV肺炎小鼠尿液和粪便生物标记物研究
Biomarkers in Urine and Feces of BALB/c Mice Infected with RSV Pneumonia Using GC-MS Technology
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摘要: 目的 通过气相色谱-质谱联用(GC-MS)技术分析RSV肺炎小鼠尿液和粪便中代谢物的相对含量变化,寻找可协助RSV肺炎诊断的生物标记物。方法 RSV滴鼻感染BALB/c小鼠建立RSV肺炎模型,分别收集小鼠的尿液和粪便,经肟化和衍生化后,运用GC-MS检测尿液和粪便中所含有的代谢物及其相对含量的变化。结果 RSV肺炎小鼠的尿液中共测得38种代谢产物,其中乳酸、丙酸、L-脯氨酸为差异代谢产物(P<0.05);粪便中共测得43种代谢物,其中亮氨酸、天冬氨酸、胱氨酸、酪氨酸、嘧啶有显著差异(P<0.05)。结论 运用GC-MS代谢组学技术寻找RSV肺炎小鼠尿液和粪便中的生物标记物具有可行性。Abstract: OBJECTIVE To detect the relative abundance changes of metabolites in urine and feces of BALB/c mice to seek the biomarkers that contribute to the diagnosis by GC-MS technology. METHODS BALB/c mice were challenged intranasally to establish the RSV pneumonia models. Urine and feces were collected respectively, oximated, derivatized and detected by GC-MS. RESULTS 38 metabolites in urine were detected, among which lactic acid, propanoic acid and L-proline were statistically significant (P<0.05). Moreover, 43 metabolites in feces were detected, and leucine, aspartic acid, cystine, tyrosine and pyrimidine had statistical significance(P<0.05). CONCLUSION GC-MS technology is feasible for seeking biomarkers in urine and feces of mice infected with RSV pneumonia.
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Key words:
- GC-MS /
- urine /
- feces /
- RSV pneumonia /
- biomarkers
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[1] HARRIS J, WERLING D. Binding and entry of respiratory syncytial virus into host cells and initiation of the innate immune response[J].Cell Microbio,2003,5(10):671-680. [2] 汪受传,韩新民,任现志,等.小儿病毒性肺炎480例中医证候学特点研究[J].南京中医药大学学报,2007,23(1):14-19. [3] WANG SC, HAN XM, REN XZ, et al. A study on TCM symptomatology based on treatment of 480 cases of children's viral pneumonia[J]. J Nanjing Univ Chin Med,2007,23(1):14-19. [4] 张丽萍,黄伟,王雪峰.王雪峰教授通腑法佐治小儿肺炎各期经验[J].中国中西医结合儿科学,2013,5(4):306-308. [5] ZHANG LP, HUANG W, WANG XF. Professor WANG Xue-feng experience treating for infantile pneumonia with Tongfu Methods[J]. Chin Pediatr Integr Tradit West Med,2013,5(4):306-308. [6] 李佳曦,汪受传,徐建亚,等.金欣口服液含药血清及其有效单体白藜芦醇对RSV的RNA聚合酶转录活性的影响[J].南京中医药大学学报,2011,27(1):36-39. [7] LI JX, WANG SC, XU JY, et al. Study on effect of Jinxin oral liquid medicated serum and effective monomers resveratrol on transcription activity of RSV's RNA polymerase[J]. J Nanjing Univ Tradit Chin Med,2011,27(1):36-39. [8] MAJOR HJ, WILLIAMS R, WILSON AJ, et al. A metabonomic analysis of plasma from Zucker rat strains using gas chromatography/mass spectrometry and pattern recognition[J].Rapid commun Mass Specrtom. 2006,20(22):3295-3302. [9] MUNGER J. Dynamics of the cellular metabolome during human cytomegalovirus infection[J]. Plos Pathogen,2006,2(12):1165-1175. [10] WEBB-ROBERTSON BJ, KIM YM, ZINK EM, et al. A Statistical Analysis of the Effects of Urease pre-treatment on the Measurement of the Urinary Metabolome by Gas Chromatography-Mass Spectrometry[J].Metabolomics.2014,10(5):897-908. [11] MURRAY DM, BOYLAN GB, FITZGERALD AP, et al. Persistent lactic acidosis in neonatal hypoxic-ischaemic encephalopathy correlates with EEG grade and electrographic seizure burden[J]. Archi Dis Childhood-Fetal Neonatal Edit, 2008,93(3): 183-186. [12] 程爱兰,韩连书,严永清.丙酸血症的脑MRI表现及1H-MRS价值分析[J].影像诊断与介入放射学,2015,24(4):313-316. [13] CHENG AL, HAN LS, YAN YQ. Value of MRI and 1H-MR spectroscopy in propionic acidemia[J]. Diagn Imag Intervent Radiol,2015,24(4):313-316. [14] 李承威,马淑霞,王春敏,等.胶原蛋白肽增强小鼠免疫力的实验研究[J].中国微生态学杂志,2013,25(4):387-389. [15] LI CW, MA SX, WANG CM, et al. Collagen peptide enhances immunity in mice[J]. Chin J Microecol,2013,25(4):387-389. [16] 李言川,赵连友,王士雯,等.肺部感染衰老大鼠血浆血管加压素浓度变化与心脏胶原蛋白的关系[J].第四军医大学学报,2004,25(8):680-683. [17] LI YC, ZHAO LY, WANG SW, et al. The relation of changes in plasma AVP concentration and myocardial collagen content during pneumonia in experimental aging rats[J].J Fourth Milit Med Univ,2004,25(8):680-683. [18] CHACKO A, CUMMINGS JH. Nitrogen losses from the human small bowel: Obligatory losses and the effect of physical form of food[J].Gut,1988,29(6):809-815. [19] ADIBI SA,MERCER DW. Protein digestion in human intestine as reflected in luminal, mucosal,and plasma amino acid concentrations after meals[J].J Clin Invest,1973, 52(7):1586-1594. [20] SUMMERSKILL WH, WOLPERT E.Ammonia metabolism in the gut[J]. Am J Clin Nutr. 1970,23(5):633-639. [21] MARTIN FPJ, WANG Y, YAP IKS, et al. Topographical variation in murine intestinal metabolic profiles in relation to microbiome speciation and functional ecological activity[J]. J Proteom Res, 2009, 8(7):3464-3474.
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