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基于GC-MS代谢组学的RSV肺炎小鼠尿液和粪便生物标记物研究

孟欣 汪受传 谢彤 徐建亚 沈存思 单进军

孟欣, 汪受传, 谢彤, 徐建亚, 沈存思, 单进军. 基于GC-MS代谢组学的RSV肺炎小鼠尿液和粪便生物标记物研究[J]. 南京中医药大学学报, 2017, 33(2): 198-206.
引用本文: 孟欣, 汪受传, 谢彤, 徐建亚, 沈存思, 单进军. 基于GC-MS代谢组学的RSV肺炎小鼠尿液和粪便生物标记物研究[J]. 南京中医药大学学报, 2017, 33(2): 198-206.
MENGXin, WANGShou-chuan, XIETong, XUJian-ya, SHENCun-si, SHANJin-jun. Biomarkers in Urine and Feces of BALB/c Mice Infected with RSV Pneumonia Using GC-MS Technology[J]. Journal of Nanjing University of traditional Chinese Medicine, 2017, 33(2): 198-206.
Citation: MENGXin, WANGShou-chuan, XIETong, XUJian-ya, SHENCun-si, SHANJin-jun. Biomarkers in Urine and Feces of BALB/c Mice Infected with RSV Pneumonia Using GC-MS Technology[J]. Journal of Nanjing University of traditional Chinese Medicine, 2017, 33(2): 198-206.

基于GC-MS代谢组学的RSV肺炎小鼠尿液和粪便生物标记物研究

Biomarkers in Urine and Feces of BALB/c Mice Infected with RSV Pneumonia Using GC-MS Technology

  • 摘要: 目的 通过气相色谱-质谱联用(GC-MS)技术分析RSV肺炎小鼠尿液和粪便中代谢物的相对含量变化,寻找可协助RSV肺炎诊断的生物标记物。方法 RSV滴鼻感染BALB/c小鼠建立RSV肺炎模型,分别收集小鼠的尿液和粪便,经肟化和衍生化后,运用GC-MS检测尿液和粪便中所含有的代谢物及其相对含量的变化。结果 RSV肺炎小鼠的尿液中共测得38种代谢产物,其中乳酸、丙酸、L-脯氨酸为差异代谢产物(P<0.05);粪便中共测得43种代谢物,其中亮氨酸、天冬氨酸、胱氨酸、酪氨酸、嘧啶有显著差异(P<0.05)。结论 运用GC-MS代谢组学技术寻找RSV肺炎小鼠尿液和粪便中的生物标记物具有可行性。

     

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出版历程
  • 收稿日期:  2016-09-25
  • 修回日期:  2016-12-25
  • 刊出日期:  2017-03-10

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